Recombinant Rat Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial (Aadat)

Code CSB-YP717557RA
MSDS
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Source Yeast
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Code CSB-EP717557RA
MSDS
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Source E.coli
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Code CSB-EP717557RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP717557RA
MSDS
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Source Baculovirus
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Code CSB-MP717557RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Aadat
Uniprot No.
Alternative Names
Aadat; Kat2Kynurenine/alpha-aminoadipate aminotransferase; mitochondrial; KAT/AadAT; 2-aminoadipate aminotransferase; 2-aminoadipate transaminase; EC 2.6.1.39; Alpha-aminoadipate aminotransferase; AadAT; Kynurenine aminotransferase II; Kynurenine--oxoglutarate aminotransferase II; Kynurenine--oxoglutarate transaminase 2; EC 2.6.1.7; Kynurenine--oxoglutarate transaminase II
Species
Rattus norvegicus (Rat)
Expression Region
30-425
Target Protein Sequence
P KDIISLAPGS PNPKVFPFKS AVFTVENGST IRFEGEMFQR ALQYSSSYGI PELLSWLKQL QIKLHNPPTV NYSPNEGQMD LCITSGCQDG LCKVFEMLIN PGDTVLVNEP LYSGALFAMK PLGCNFISVP SDDCGIIPEG LKKVLSQWKP EDSKDPTKRT PKFLYTIPNG NNPTGNSLTG DRKKEIYELA RKYDFLIIED DPYYFLQFTK PWEPTFLSMD VDGRVIRADS LSKVISSGLR VGFITGPKSL IQRIVLHTQI SSLHPCTLSQ LMISELLYQW GEEGFLAHVD RAIDFYKNQR DFILAAADKW LRGLAEWHVP KAGMFLWIKV NGISDAKKLI EEKAIEREIL LVPGNSFFVD NSAPSSFFRA SFSQVTPAQM DLVFQRLAQL IKDVS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino-group acceptors, with a preference for 2-oxoglutarate, 2-oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro).
Gene References into Functions
  1. neuronal culture showed positive immunostaining for both kynurenic acid aminotransferase (KAT) isoforms I and II PMID: 15880762
  2. identification of presence in the permanent immature rat oligodendrocyte cell line OLN-93 PMID: 16258845
  3. We found specific staining of KAT II-positive astrocyte-like cells throughout the adult rat brain. The presence of KAT II in astrocytes was confirmed by double fluorescence immunostaining with an antibody against the astrocyte-specific marker GFAP. PMID: 17024659
Subcellular Location
Mitochondrion.
Protein Families
Class-I pyridoxal-phosphate-dependent aminotransferase family
Database Links
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