Recombinant Rat Phosphatidylethanolamine-binding protein 1 (Pebp1)

Code CSB-YP017766RA
MSDS
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Source Yeast
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Code CSB-EP017766RA
MSDS
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Source E.coli
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Code CSB-EP017766RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017766RA
MSDS
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Source Baculovirus
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Code CSB-MP017766RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pebp1
Uniprot No.
Alternative Names
Pebp1; Pbp; Pebp; Phosphatidylethanolamine-binding protein 1; PEBP-1; 23 kDa morphine-binding protein; HCNPpp; P23K) [Cleaved into: Hippocampal cholinergic neurostimulating peptide; HCNP)]
Species
Rattus norvegicus (Rat)
Expression Region
2-187
Target Protein Sequence
AADISQWAG PLSLQEVDEP PQHALRVDYG GVTVDELGKV LTPTQVMNRP SSISWDGLDP GKLYTLVLTD PDAPSRKDPK FREWHHFLVV NMKGNDISSG TVLSEYVGSG PPKDTGLHRY VWLVYEQEQP LNCDEPILSN KSGDNRGKFK VESFRKKYHL GAPVAGTCFQ AEWDDSVPKL HDQLAGK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase. Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation.; HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor.
Gene References into Functions
  1. RKIP attenuates ischemia-induced microglia cell apoptosis and activation through NF-kappaB pathway. PMID: 28245468
  2. Loss of RKIP may be associated with malignant progression in hepatic fibrosis. PMID: 27470410
  3. Raf-1 kinase inhibitory protein plays an important role in the neural cell apoptosis induced by microwave radiation PMID: 25108669
  4. RKIP overexpression increases cardiac contractility mediated by the beta1-adrenoceptor. RKIP deficiency exaggerates pressure overload-induced cardiac failure. PMID: 26479924
  5. PEBP1, given its synaptic localization and its role as a signaling molecule acting via the ERK/MAPK pathway, could be a potential therapeutic target mediating drug-seeking behaviors associated with neuroinflammation. PMID: 25962787
  6. LTD4 inhibits ASCT1 through PKC-mediated phosphorylation of RKIP, leading to the subsequent activation of PKA pathway, possibly through beta2-andrenergic receptor activation. PMID: 25231108
  7. These results suggest that expression levels of RKIP might be regulated by RTX via NF-kappaB PMID: 24065653
  8. Raf-1 kinase inhibitory protein (RKIP) mediates ethanol-induced sensitization of secretagogue signaling in pancreatic acinar cells PMID: 22859298
  9. These findings suggest that HCNP, in association with CRMP-2, plays an important role in presynaptic function in the hippocampus PMID: 22542739
  10. RKIP in the hippocampus is downregulated by electromagnetic radiation. PMID: 19309582
  11. Decreased PEBP1 expression correlates with subsequent activation of mitogen-activated protein (MAP) kinase signaling components of the MAP kinase cascade. PMID: 21554319
  12. Study suggest that PEBP and C11orf59 might be involved in controlling lipid or carbohydrate metabolism in diabetes via PPARalpha activation. PMID: 20510017
  13. Data suggest that lowered expression of PEBP1 might be responsible for dementia associated with high-altitude hypoxia. PMID: 19705086
  14. The excessive activation of RKIP mediated ERK pathway is one of the important mechanisms for the apoptosis and death of hippocampus neurons induced by electromagnetic radiation. PMID: 18616905
  15. selective modulation of c-Raf-1 but not B-Raf activation by RKIP can limit the dynamic range of the MAPK signaling response to growth factors and may play a critical role in growth and development PMID: 15886202
  16. These results indicate that RKIP regulates Aurora B kinase and the spindle checkpoint via the Raf-1/MEK/ERK cascade and demonstrate that small changes in the MAP kinase (MAPK) pathway can profoundly impact the fidelity of the cell cycle. PMID: 16916643
  17. PEBP1 may be a novel molecular mediator influencing cognitive integrity during chronic corticosterone exposure in rat hippocampus. PMID: 18311602
  18. Raf kinase inhibitor protein inhibits ERK/MAPK signalling and this inhibition impedes HSC proliferation. RKIP promotes HSC migration and wound closure. PMID: 19323783
  19. Receptor distribution in crude brain membrane fractions and similarity in distribution patterns between binding site and HCNP-precursor protein mRNA expression suggest that HCNP exerts its functions near the dendrites of the neurons that produce it. PMID: 12591138

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Subcellular Location
Cytoplasm. Membrane; Peripheral membrane protein.
Protein Families
Phosphatidylethanolamine-binding protein family
Tissue Specificity
Major component of epididymal secretions and sperm plasma membranes. It is present in cytosols from a variety of other tissues. Highly expressed in brain.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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