Recombinant Rat Sialidase-3 (Neu3)

Code CSB-YP857526RA
MSDS
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Source Yeast
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Code CSB-EP857526RA
MSDS
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Source E.coli
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Code CSB-EP857526RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP857526RA
MSDS
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Source Baculovirus
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Code CSB-MP857526RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Neu3
Uniprot No.
Alternative Names
Neu3; Sialidase-3; EC 3.2.1.18; Ganglioside sialidase; Membrane sialidase; N-acetyl-alpha-neuraminidase 3
Species
Rattus norvegicus (Rat)
Expression Region
1-418
Target Protein Sequence
MEEVSSCSLR STLFQQEEQN RITYRIPALL YIPPTHTFLA FAEMRTSSRD EDAVYLVFRR GVMKGCSVEW GPQQPLMEAT LPGHRTMSPC PVWEKNTGRV YLFFICVQGH VSERWQLLWG RNAARLCFLY SEDSGCSWGE VKDLTEEVVG SEMKHWATFA VGPGHGIQLQ SGRLLIPAYA YLISCWFLCF PCSVKPHSLM FYSDDLGVTW HCGKFIKPQV TGECQVAEVP GKAGNLVLYC SARTPNKFRA EAFSTDSGDC FQKPTLNQQL CEPRGGCQGS VVSTRPLKMP YTCQDSSGKD VPSTQKCPLM DRSLEVEEGA GAPSGTWLLY SHPTNKKKRM NLGIYYNQNP LEVNYWSRPW ILNRGPSGYS DLAVVEGQGL FACLFECGER HEDEKIDFCL FSDQEVLSCD DCTSPSSN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Exo-alpha-sialidase that catalyzes the hydrolytic cleavage of the terminal sialic acid (N-acetylneuraminic acid, Neu5Ac) of a glycan moiety in the catabolism of glycolipids, glycoproteins and oligosacharides. Displays high catalytic efficiency for gangliosides including alpha-(2->3)-sialylated GD1a and GM3 and alpha-(2->8)-sialylated GD3. Plays a role in the regulation of transmembrane signaling through the modulation of ganglioside content of the lipid bilayer and by direct interaction with signaling receptors, such as EGFR. Desialylates EGFR and activates downstream signaling in proliferating cells. Contributes to clathrin-mediated endocytosis by regulating sorting of endocytosed receptors to early and recycling endosomes.
Gene References into Functions
  1. findings implicate NEU3 as a potential biomarker of insulin sensitivity, and provide novel mechanistic insight into the regulation of NEU3 expression. PMID: 26022181
  2. activation of Neu3 sialidase is an essential step in regeneration occurring in PNS (sensory) but not CNS (retinal) axons PMID: 24523539
  3. NEU3 participates in the control of insulin signaling via modulation of gangliosides and interaction with Grb2 PMID: 12730204
Subcellular Location
Cell membrane; Peripheral membrane protein. Membrane, caveola. Early endosome membrane; Peripheral membrane protein. Recycling endosome membrane; Peripheral membrane protein. Lysosome membrane; Peripheral membrane protein.
Protein Families
Glycosyl hydrolase 33 family
Tissue Specificity
Expressed in brain, cardiac muscle and weakly in liver.
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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