Recombinant Response regulator gacA (gacA)

Code CSB-YP341378FFT
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP341378FFT
MSDS
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Source E.coli
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Code CSB-EP341378FFT-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP341378FFT
MSDS
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Source Baculovirus
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Code CSB-MP341378FFT
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
gacA
Uniprot No.
Alternative Names
gacA; Response regulator GacA; Global activator; Global antibiotic and cyanide control protein
Species
Pseudomonas protegens (strain DSM 19095 / LMG 27888 / CHA0)
Expression Region
1-213
Target Protein Sequence
MIRVLVVDDH DLVRTGITRM LADIDGLQVV GQAESGEESL LKARELKPYV VLMDVKMPGI GGLEATRKLL RSHPDIKVVA VTVCEEDPFP TRLLQAGAAG YLTKGAGLNE MVQAIRLVFA GQRYISPQIA QQLVFKSFQP SSDSPFDALS EREIQIALMI VGCQKVQIIS DKLCLSPKTV NTYRYRIFEK LSISSDVELT LLAVRHGMVD ASL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Member of the two-component regulatory system GacA/GacS which controls the expression of secondary metabolites and extracellular products. Acts (probably primarily) by activating expression of CsrA1 and CsrA2 antagonist small RNAs (sRNA) RsmX, RsmY and RsmZ which bind to and prevent translation repression by CsrA1 and CsrA2. Involved in the regulation of secondary metabolism and in the synthesis of the antifungal factors cyanide, 2,4-diacetylphloroglucinol and pyoluteorin. Involved in synthesis of the autoinducing signal (unrelated to N-acylhomoserine lactones, induces the Gac/Csr cascade). Exercises positive post-transcriptional control over the hcnABC and aprA genes; acts upstream of CsrA2 (rsmA). Controls expression of csrA1 (rsmE) and csrA2.
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