Recombinant Rhizobium sp. Probable ATP synthase y4yI (NGR_a00640)

Code CSB-YP346285RKX
MSDS
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Source Yeast
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Code CSB-EP346285RKX
MSDS
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Source E.coli
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Code CSB-EP346285RKX-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP346285RKX
MSDS
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Source Baculovirus
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Code CSB-MP346285RKX
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NGR_a00640
Uniprot No.
Alternative Names
NGR_a00640; y4yI; Probable ATP synthase y4yI; EC 7.1.2.2
Species
Sinorhizobium fredii (strain NBRC 101917 / NGR234)
Expression Region
1-451
Target Protein Sequence
MITPVPQHNS LEGTPLEPAI SSLWSTAKRI DTRVVRGRIT RAVGTLIHAV LPEARIGELC LLQDTRTGLS LEAEVIGLLD NGVLLTPIGG LAGLSSRAEV VSTGRMREVP IGPDLLGRVI DSRCRPLDGK GEVKTTEVRP LHGRAPNPMT RRMVERPFPL GVRALDGLLT CGEGQRIGIY GEPGGGKSTL ISQIVKGAAA DVVIVALIGE RGREVREFVE RHLGEEGLRR AIVVVETSDR SATERAQCAP MATALAEYFR EQGLRVALLL DSLTRFCRAM REIGLAAGEP PTRRGFPPSV FAALPGLLER AGLGERGSIT AFYTVLVEGD GTGDPIAEES RGILDGHIVL SRALAARSHF PAIDVLQSRS RVMDAVVSET HRKAASFFRD LLARYAECEF LINVGEYKQG GDPLTDRAVA SIGELKEFLR QSEDEVSDFE ETVGWMSRLT S
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
ATPase component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells. Acts as a molecular motor to provide the energy that is required for the export of proteins. Required for type III secretion apparatus (T3SA) formation, proper protein secretion, host cell invasion and virulence. May play a critical role in T3SS substrate recognition, disassembly of the effector/chaperone complex and unfolding of the effector in an ATP-dependent manner prior to secretion.
Subcellular Location
Cytoplasm.
Protein Families
ATPase alpha/beta chains family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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