Recombinant Rhodobacter sphaeroides Ketol-acid reductoisomerase(ilvC)

Product Type Recombinant Protein
Uniprot No. A4WQ93
Species Rhodobacter sphaeroides (strain ATCC 17025 / ATH 2.4.3)
Gene Names ilvC
Protein Names Recommended name: Ketol-acid reductoisomerase EC= 1.1.1.86 Alternative name(s): Acetohydroxy-acid isomeroreductase Alpha-keto-beta-hydroxylacil reductoisomerase
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.
Protein Families Ketol-acid reductoisomerase family
Database Links

KEGG: rsq:Rsph17025_0651

STRING: 349102.Rsph17025_0651

Product Details for Different Expression Systems

Product Name Recombinant Rhodobacter sphaeroides Ketol-acid reductoisomerase(ilvC),Yeast
Code CSB-YP396415RLG
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MRVYYDRDCD INLIKDKKVA ILGYGSQGHA HALNLRDSGA KNVVVALREG SPSAKKAEAE GLKVMGIAEA AAWCDLIMFT MPDELQAETY KKYVHDNLKE GSAIAFAHGL NVHFGLIEPK PGVDVIMMAP KGPGHTVRGE YVKGGGVPCL VAVHNDATGK AMEIGLSYCS AIGGGRSGII ETNFRQECET DLFGEQAVLC GGLVELIRMG FETLVEAGYE PEMAYFECLH EVKLIVDLIY EGGIANMNYS ISNTAEYGEY VSGPRILPYE ETKARMKAVL TDIQTGKFVR DFMQENAVGQ PFFKATRRIN DEHQIEKVGE KLRGMMPWIS KGKMVDRARN
Expression Region 1-340
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Rhodobacter sphaeroides Ketol-acid reductoisomerase(ilvC),E.coli
Code CSB-EP396415RLG
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MRVYYDRDCD INLIKDKKVA ILGYGSQGHA HALNLRDSGA KNVVVALREG SPSAKKAEAE GLKVMGIAEA AAWCDLIMFT MPDELQAETY KKYVHDNLKE GSAIAFAHGL NVHFGLIEPK PGVDVIMMAP KGPGHTVRGE YVKGGGVPCL VAVHNDATGK AMEIGLSYCS AIGGGRSGII ETNFRQECET DLFGEQAVLC GGLVELIRMG FETLVEAGYE PEMAYFECLH EVKLIVDLIY EGGIANMNYS ISNTAEYGEY VSGPRILPYE ETKARMKAVL TDIQTGKFVR DFMQENAVGQ PFFKATRRIN DEHQIEKVGE KLRGMMPWIS KGKMVDRARN
Expression Region 1-340
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Rhodobacter sphaeroides Ketol-acid reductoisomerase(ilvC),Baculovirus
Code CSB-BP396415RLG
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MRVYYDRDCD INLIKDKKVA ILGYGSQGHA HALNLRDSGA KNVVVALREG SPSAKKAEAE GLKVMGIAEA AAWCDLIMFT MPDELQAETY KKYVHDNLKE GSAIAFAHGL NVHFGLIEPK PGVDVIMMAP KGPGHTVRGE YVKGGGVPCL VAVHNDATGK AMEIGLSYCS AIGGGRSGII ETNFRQECET DLFGEQAVLC GGLVELIRMG FETLVEAGYE PEMAYFECLH EVKLIVDLIY EGGIANMNYS ISNTAEYGEY VSGPRILPYE ETKARMKAVL TDIQTGKFVR DFMQENAVGQ PFFKATRRIN DEHQIEKVGE KLRGMMPWIS KGKMVDRARN
Expression Region 1-340
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Rhodobacter sphaeroides Ketol-acid reductoisomerase(ilvC),Mammalian cell
Code CSB-MP396415RLG
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MRVYYDRDCD INLIKDKKVA ILGYGSQGHA HALNLRDSGA KNVVVALREG SPSAKKAEAE GLKVMGIAEA AAWCDLIMFT MPDELQAETY KKYVHDNLKE GSAIAFAHGL NVHFGLIEPK PGVDVIMMAP KGPGHTVRGE YVKGGGVPCL VAVHNDATGK AMEIGLSYCS AIGGGRSGII ETNFRQECET DLFGEQAVLC GGLVELIRMG FETLVEAGYE PEMAYFECLH EVKLIVDLIY EGGIANMNYS ISNTAEYGEY VSGPRILPYE ETKARMKAVL TDIQTGKFVR DFMQENAVGQ PFFKATRRIN DEHQIEKVGE KLRGMMPWIS KGKMVDRARN
Expression Region 1-340
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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