Recombinant Saccharomyces cerevisiae PHO85 cyclin-5 (PCL5)

Code CSB-YP334477SVG
MSDS
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Source Yeast
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Code CSB-EP334477SVG
MSDS
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Source E.coli
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Code CSB-EP334477SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP334477SVG
MSDS
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Source Baculovirus
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Code CSB-MP334477SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PCL5
Uniprot No.
Alternative Names
PCL5; YHR071W; H8025.1; PHO85 cyclin-5; G1/S-specific cyclin PCL5
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-229
Target Protein Sequence
MDGNHRFTPD SKEFNTVVKS KESSTGRNPY QTPPLEHNGT HHQTNYSRKK TNLAIIISNF LSEISRPLSN GKINNSTHNI LKFLNEVLKR SKCSKENAVL ATFYFQKIHQ SRGVRDESSL PEFSHCSRRI FLCCLILSHK FLNDNTYSMK NWQIISGLHA KDLSLMERWC LGKLNYELAI PYDEFLLWET NTLMKAKLRV GTPANAPVKR PRESDNDYDA NSWKQIKSC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Cyclin partner of the cyclin-dependent kinase (CDK) PHO85. Positively controls degradation of transcription factor GCN4 under favorable growth conditions. The PCL5-PHO85 cyclin-CDK holoenzyme phosphorylates GCN4, which is required for its degradation by the E3 ubiquitin ligase complex SCF(Cdc4). Amino acid starvation reduces PCL5-PHO85-associated GCN4 kinase activity and leads to stabilization of GCN4.
Gene References into Functions
  1. Localization of Pcl5 into the cytoplasm prevents the degradation of Gcn4. PMID: 19218424
Protein Families
Cyclin family, PCL1,2 subfamily
Database Links

KEGG: sce:YHR071W

STRING: 4932.YHR071W

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