Recombinant Saccharomyces cerevisiae Protein IMPACT homolog (YIH1)

Code CSB-YP335139SVG
MSDS
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Source Yeast
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Code CSB-EP335139SVG
MSDS
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Source E.coli
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Code CSB-EP335139SVG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP335139SVG
MSDS
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Source Baculovirus
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Code CSB-MP335139SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
YIH1
Uniprot No.
Alternative Names
YIH1; YCR059C; YCR59C; Protein IMPACT homolog
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-258
Target Protein Sequence
MDDDHEQLVE ELEAVEAIYP DLLSKKQEDG SIIVVKVPQH EYMTLQISFP THYPSEEAPN VIEVGVCTSL AKRDLYDTKY LQHLFQEVMD SVFHRGSVCL FDFLTELDGV LYVEPEEETE PVQQSDIPTD PFEGWTASDP ITDRGSTFMA FAAHVTSEEQ AFAMLDLLKT DSKMRKANHV MSAWRIKQDG SAATYQDSDD DGETAAGSRM LHLITIMDVW NVIVVVARWF GGAHIGPDRF KHINSTAREA VVRAGFDS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Translational regulator that ensures constant high levels of translation under amino acid starvation. Plays a role as a negative regulator of the GCN2 kinase activity; impairs GCN1-mediated GCN2 activation, and hence GCN2-mediated eIF-2-alpha phosphorylation in amino acid-starved cells and subsequent down-regulation of protein synthesis. In normal conditions, it resides in a actin complex and has no activity.
Gene References into Functions
  1. Yih1 modulates the function of Cdc28 in specific stages of the cell cycle. PMID: 26176233
  2. Yih1 associates with ribosomes and that these ribosomes may simultaneously carry Gcn1 and Gcn2. PMID: 22404850
  3. Yih1 binds with different requirements to distinct populations of Gcn1 molecules, and its ability to disrupt Gcn1-Gcn2 complexes is dependent on a complete RWD and hindered by actin binding. PMID: 21239490
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
IMPACT family
Database Links

KEGG: sce:YCR059C

STRING: 4932.YCR059C

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