Recombinant Saccharomyces cerevisiae mRNA transport regulator MTR2 (MTR2)

Code CSB-YP335908SVG
MSDS
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Source Yeast
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Code CSB-EP335908SVG
MSDS
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Source E.coli
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Code CSB-EP335908SVG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP335908SVG
MSDS
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Source Baculovirus
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Code CSB-MP335908SVG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MTR2
Uniprot No.
Alternative Names
MTR2; YKL186C; mRNA transport regulator MTR2
Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Expression Region
1-184
Target Protein Sequence
MNTNSNTMVM NDANQAQITA TFTKKILAHL DDPDSNKLAQ FVQLFNPNNC RIIFNATPFA QATVFLQMWQ NQVVQTQHAL TGVDYHAIPG SGTLICNVNC KVRFDESGRD KMGQDATVPI QPNNTGNRNR PNDMNKPRPL WGPYFGISLQ LIIDDRIFRN DFNGVISGFN YNMVYKPEDS LLKI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Affects mRNA transport from the nucleus to the cytoplasm.
Gene References into Functions
  1. Mex67-Mtr2 functions in primary nuclear export for a subset of yeast tRNAs PMID: 29212662
  2. This study describes the mechanism of recruitment of the Saccharomyces cerevisiae RNA-export receptor Mex67-Mtr2 to the pre-60S subunit at the proper time. Mex67-Mtr2 binds at the premature ribosomal-stalk region, which later during translation serves as a binding platform for translational GTPases on the mature ribosome. PMID: 27775710
  3. show that the NTF2-like domain from Saccharomyces cerevisiae Mex67:Mtr2 also contributes to RNA binding PMID: 25618852
  4. Thus Mex67-Mtr2 is a unique transport receptor that employs a common interaction surface to participate in the nuclear export of both pre-ribosomal subunits and mRNAs PMID: 22956913
  5. Thus, the general mRNA exporter Mex67-Mtr2 contains a distinct electrostatic interaction surface for transporting 60S preribosomal cargo. PMID: 17434126
  6. Mex67-Mtr2 export receptor employs a versatile binding platform on its surface that can create a crosstalk between mRNA and ribosome export pathways. PMID: 18046452

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Subcellular Location
Nucleus.
Database Links

KEGG: sce:YKL186C

STRING: 4932.YKL186C

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