Recombinant Saccharopolyspora erythraea 3-isopropylmalate dehydrogenase(leuB)

Product Type Recombinant Protein
Uniprot No. A4FMQ2
Species Saccharopolyspora erythraea (strain ATCC 11635 / DSM 40517 / JCM 4748 / NBRC 13426 / NCIMB 8594 / NRRL 2338)
Gene Names leuB
Protein Names Recommended name: 3-isopropylmalate dehydrogenase EC= 1.1.1.85 Alternative name(s): 3-IPM-DH Beta-IPM dehydrogenase Short name= IMDH
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
Subcellular Location Cytoplasm
Protein Families Isocitrate and isopropylmalate dehydrogenases family, LeuB type 2 subfamily
Database Links

KEGG: sen:SACE_6154

STRING: 405948.SeryN2_010100009404

Product Details for Different Expression Systems

Product Name Recombinant Saccharopolyspora erythraea 3-isopropylmalate dehydrogenase(leuB),Yeast
Code CSB-YP389424STB
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MRLAVIPGDG IGPEVVAEAL KVLGEVVPDT EITRYDLGAA RWHATGELLP ESVLGELRQH DAILLGAVGD PSVPSGILER GLLLRLRFEL DHHVNLRPAR LYPGVKSPVS DPGEIDMVVV REGTEGPYAG NGGLLRKDTT HEIATEVSIN TAFGVERVVR DAFARAASRP RKHLTLVHKT NVLTHAGSLW SRVVEEVSLQ HPDVTVAYQH VDATTIHMVT DPSRFDVIVT DNLFGDIITD LAGAITGGIG LAASGNIDAT RRNPSMFEPV HGSAPDIAGQ GTADPTAAVL SVALLLDHVG QSEAARRVEA AVAFDLATRD HSAPGATFAI GDRLAALVSS REVAQQA
Expression Region 1-347
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
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Product Name Recombinant Saccharopolyspora erythraea 3-isopropylmalate dehydrogenase(leuB),E.coli
Code CSB-EP389424STB
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MRLAVIPGDG IGPEVVAEAL KVLGEVVPDT EITRYDLGAA RWHATGELLP ESVLGELRQH DAILLGAVGD PSVPSGILER GLLLRLRFEL DHHVNLRPAR LYPGVKSPVS DPGEIDMVVV REGTEGPYAG NGGLLRKDTT HEIATEVSIN TAFGVERVVR DAFARAASRP RKHLTLVHKT NVLTHAGSLW SRVVEEVSLQ HPDVTVAYQH VDATTIHMVT DPSRFDVIVT DNLFGDIITD LAGAITGGIG LAASGNIDAT RRNPSMFEPV HGSAPDIAGQ GTADPTAAVL SVALLLDHVG QSEAARRVEA AVAFDLATRD HSAPGATFAI GDRLAALVSS REVAQQA
Expression Region 1-347
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Saccharopolyspora erythraea 3-isopropylmalate dehydrogenase(leuB),Baculovirus
Code CSB-BP389424STB
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MRLAVIPGDG IGPEVVAEAL KVLGEVVPDT EITRYDLGAA RWHATGELLP ESVLGELRQH DAILLGAVGD PSVPSGILER GLLLRLRFEL DHHVNLRPAR LYPGVKSPVS DPGEIDMVVV REGTEGPYAG NGGLLRKDTT HEIATEVSIN TAFGVERVVR DAFARAASRP RKHLTLVHKT NVLTHAGSLW SRVVEEVSLQ HPDVTVAYQH VDATTIHMVT DPSRFDVIVT DNLFGDIITD LAGAITGGIG LAASGNIDAT RRNPSMFEPV HGSAPDIAGQ GTADPTAAVL SVALLLDHVG QSEAARRVEA AVAFDLATRD HSAPGATFAI GDRLAALVSS REVAQQA
Expression Region 1-347
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Saccharopolyspora erythraea 3-isopropylmalate dehydrogenase(leuB),Mammalian cell
Code CSB-MP389424STB
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MRLAVIPGDG IGPEVVAEAL KVLGEVVPDT EITRYDLGAA RWHATGELLP ESVLGELRQH DAILLGAVGD PSVPSGILER GLLLRLRFEL DHHVNLRPAR LYPGVKSPVS DPGEIDMVVV REGTEGPYAG NGGLLRKDTT HEIATEVSIN TAFGVERVVR DAFARAASRP RKHLTLVHKT NVLTHAGSLW SRVVEEVSLQ HPDVTVAYQH VDATTIHMVT DPSRFDVIVT DNLFGDIITD LAGAITGGIG LAASGNIDAT RRNPSMFEPV HGSAPDIAGQ GTADPTAAVL SVALLLDHVG QSEAARRVEA AVAFDLATRD HSAPGATFAI GDRLAALVSS REVAQQA
Expression Region 1-347
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Still Have Questions? Leave a Message or Start an on-line Chat

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