Recombinant Shewanella baltica DNA replication and repair protein recF(recF)

Code CSB-YP433743STN
Size Pls inquire other sizes
Source Yeast
Code CSB-EP433743STN
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Source E.coli
Code CSB-EP433743STN-B
Size Pls inquire other sizes
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Code CSB-BP433743STN
Size Pls inquire other sizes
Source Baculovirus
Code CSB-MP433743STN
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Source Mammalian cell

Product Details

Purity >85% (SDS-PAGE)
Target Names recF
Uniprot No. A9KU74
Species Shewanella baltica (strain OS195)
Expression Region 1-360
Target Protein Sequence MSLTRLNIEA FRNIQSAQLI PAPGINLIYG QNGSGKTSIL EAIYFLGMGR SFRSHLSQRV INNDDDKLTL FATLNLARGD SKIGLRRFRS GETEVRIDGE KVKRLSTLAE TLPIQVITPE SFSLLFEGPK SRRQFIDWGA FHADPQFYGA WTNVRRVLKQ RNQLLRNGSA YSNIQFWDQE FVRYAEQVTE IRNHYVDSLN ELLKGIIGEF LPSVDVKVSF TRGWDSKTDF AELLENQYSR DLATGHTVSG PHKADLRLRV GTLPAQDALS RGQLKLLVCA LRIAQGKLLK QQIDKHSIYL VDDLPSELDA QHRQLLLKQL TDTGAQVFVT AIDPAAIVDS LHTPPSRMFH VEQGRVTVIE
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.
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Target Data

Function The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP.
Subcellular Location Cytoplasm
Protein Families RecF family
Database Links

KEGG: sbn:Sbal195_0003

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