Recombinant Staphylococcus aureus Sortase A (srtA), partial

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Code CSB-EP3093FLF
Size $388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Greater than 85% as determined by SDS-PAGE.
Uniprot No.
Research Area
Alternative Names
srtA; SAOUHSC_02834; Sortase A; Surface protein sorting A
Staphylococcus aureus (strain NCTC 8325)
Expression Region
Target Protein Sequence
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
24.9 kDa
Protein Length
Tag Info
N-terminal 6xHis-tagged
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

The region for expressing recombinant Staphylococcus aureus (strain NCTC 8325) srtA contains amino acids 25-206. The calculated molecular weight for this srtA protein is 24.9 kDa. This protein is generated in a e.coli-based system. The srtA gene fragment has been modified by fusing the N-terminal 6xHis tag, providing convenience in detecting and purifying the recombinant srtA protein during the following stages.

Staphylococcus aureus sortase A (SrtA) is an enzyme crucial for viral virulence and pathogenesis. SrtA is a transpeptidase that anchors surface proteins to the bacterial cell wall by catalyzing the covalent attachment of these proteins to peptidoglycan. This process is known as sortase-mediated anchoring and is essential for the display of virulence factors on the bacterial surface. The anchored proteins play a role in host-cell adhesion, immune evasion, and biofilm formation. Due to its importance in S. aureus pathogenicity, SrtA has been explored as a potential target for developing antimicrobial strategies against staphylococcal infections.

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Target Background

Transpeptidase that anchors surface proteins to the cell wall. Recognizes and modifies its substrate by proteolytic cleavage of a C-terminal sorting signal. Following cleavage, a covalent intermediate is formed via a thioester bond between the sortase and its substrate, which is then transferred and covalently attached to the cell wall. This sortase recognizes a Leu-Pro-x-Thr-Gly (LPXTG) motif, which is cleaved by the sortase between the threonine and glycine residues. Utilizes lipid II as the peptidoglycan substrate for the sorting reaction. Responsible for the display of important virulence factors. Important for interactions with the host and host colonization during infection.
Subcellular Location
Cell membrane; Single-pass type II membrane protein.
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