Recombinant Staphylococcus haemolyticus Oxygen regulatory protein nreC (nreC)

Code CSB-YP680606SBAH
MSDS
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Source Yeast
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Code CSB-EP680606SBAH
MSDS
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Source E.coli
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Code CSB-EP680606SBAH-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP680606SBAH
MSDS
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Source Baculovirus
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Code CSB-MP680606SBAH
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
nreC
Uniprot No.
Alternative Names
nreC; SH0660; Oxygen regulatory protein NreC; Nitrogen regulation protein C
Species
Staphylococcus haemolyticus (strain JCSC1435)
Expression Region
1-217
Target Protein Sequence
MKIVIADDHA VVRTGFSMIL NFQEDMEVVA TAADGGEAYQ KVMEHKPDVL IMDLSMPPGE SGLIATSKIS ESFPETKILI LTMYDDEEYL FHVLRNGAKG YILKNAPDEQ LLLAVRTVYK GETYVDMKLT TSLVNEFVNH KHSDDVSTND PFKILSKREL EILPLIAKGY GNKDIAEKLF VSVKTVEAHK THIMTKLDLK SKPELVEYAL KKKLLDF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Member of the two-component regulatory system NreB/NreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. Phosphorylated NreC binds to a GC-rich palindromic sequence at the promoters of the nitrate (narGHJI) and nitrite (nir) reductase operons, as well as the putative nitrate transporter gene narT, and activates their expression.
Subcellular Location
Cytoplasm.
Database Links

KEGG: sha:SH0660

STRING: 279808.SH0660

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