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Recombinant Sulfolobus islandicus rod-shaped virus 1 Holliday junction resolvase homolog (hjc)

Recombinant Sulfolobus islandicus rod-shaped virus 1 Holliday junction resolvase homolog (hjc)

Code	CSB-YP858484FPH
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP858484FPH
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP858484FPH-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP858484FPH
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP858484FPH
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

hjc

Uniprot No.

Q98VP9

Alternative Names

hjc; 121Holliday junction resolvase; EC 3.1.22.4

Species

Sulfolobus islandicus rod-shaped virus 1 (SIRV-1) (Sulfolobus virus SIRV-1)

Expression Region

1-121

Target Protein Sequence

MNIRQSGKYY EYKTLEILEK NGFKALRIPV SGTGKQALPD LIATKNNTIY PIEVKSTSKD VVTVRNFQIE KLFKFCEIFN FCECHPLVTV YYKKYKIVIV YELSQDVRTK EKIKFKYGIN S

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

A structure-specific endonuclease that binds and cleaves the four-way Holliday junctions in DNA created during repair and rearrangement by the ubiquitous process of homologous recombination. Introduces paired nicks in opposing strands; on immobile junction cleaves 2 bases 3' of the junction point, on mobile strands the cleavage site varies. Cleaves Y and loop-out structures with much lower efficiency. Binds 4-way junction DNA but not sequence-identical linear dsDNA.

Protein Families

Holliday junction resolvase Hjc family, Hje subfamily

Database Links

KEGG: vg:951380

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