Recombinant Thermoanaerobacter sp. (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB

Product Type Recombinant Protein
Uniprot No. B0K1A1
Species Thermoanaerobacter sp. (strain X514)
Gene Names miaB
Protein Names Recommended name: (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB EC= 2.-.-.- Alternative name(s): tRNA-i(6)A37 methylthiotransferase
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine.
Subcellular Location Cytoplasm
Protein Families Methylthiotransferase family, MiaB subfamily
Database Links

KEGG: tex:Teth514_1610

Product Details for Different Expression Systems

Product Name Recombinant Thermoanaerobacter sp. (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB,Yeast
Code CSB-YP534628TMJ
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MPTNIYVTEE ELKKQEKIMK EIAEENKGKN LYYHIETYGC QMNVHDSEKL AGMLEKMGYK YTENLEQADV LLFNTCAVRE HAEIRVLGRV SQMKELKARN PNLIIGVSGC MMQEKNVVEA IKEKYSYIDI VFGTHNIYKF PQLLWEALNS QDIVIDIIED TKNVIEELPV KRDSNLKAWV NIIYGCNNFC TYCIVPYTRG REKSRKPEDI IAEVKELAQK GYKEITLLGQ NVNSYGKDLD EDITFAKLLY KLNDIEGIER IRFMTSHPKD ISDELIYAMR DLDKVCEHLH LPVQAGSNKI LKKMNRKYTK EHYLEIIDKV RSNIPDIAIT TDIIVGFPGE TEEDFLETLD LVERVRFDAA YTFIYSKRAG TVAANMPDQV DDAVKHERLE RLIELQNKIS LEKSAELRGK IVEVLIEGIS KRDSNKLTSR TRTNKVVHFV GDESLIGKLA NVKITETKAW TMQGELVEVI R
Expression Region 1-471
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
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Product Name Recombinant Thermoanaerobacter sp. (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB,E.coli
Code CSB-EP534628TMJ
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MPTNIYVTEE ELKKQEKIMK EIAEENKGKN LYYHIETYGC QMNVHDSEKL AGMLEKMGYK YTENLEQADV LLFNTCAVRE HAEIRVLGRV SQMKELKARN PNLIIGVSGC MMQEKNVVEA IKEKYSYIDI VFGTHNIYKF PQLLWEALNS QDIVIDIIED TKNVIEELPV KRDSNLKAWV NIIYGCNNFC TYCIVPYTRG REKSRKPEDI IAEVKELAQK GYKEITLLGQ NVNSYGKDLD EDITFAKLLY KLNDIEGIER IRFMTSHPKD ISDELIYAMR DLDKVCEHLH LPVQAGSNKI LKKMNRKYTK EHYLEIIDKV RSNIPDIAIT TDIIVGFPGE TEEDFLETLD LVERVRFDAA YTFIYSKRAG TVAANMPDQV DDAVKHERLE RLIELQNKIS LEKSAELRGK IVEVLIEGIS KRDSNKLTSR TRTNKVVHFV GDESLIGKLA NVKITETKAW TMQGELVEVI R
Expression Region 1-471
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Thermoanaerobacter sp. (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB,Baculovirus
Code CSB-BP534628TMJ
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MPTNIYVTEE ELKKQEKIMK EIAEENKGKN LYYHIETYGC QMNVHDSEKL AGMLEKMGYK YTENLEQADV LLFNTCAVRE HAEIRVLGRV SQMKELKARN PNLIIGVSGC MMQEKNVVEA IKEKYSYIDI VFGTHNIYKF PQLLWEALNS QDIVIDIIED TKNVIEELPV KRDSNLKAWV NIIYGCNNFC TYCIVPYTRG REKSRKPEDI IAEVKELAQK GYKEITLLGQ NVNSYGKDLD EDITFAKLLY KLNDIEGIER IRFMTSHPKD ISDELIYAMR DLDKVCEHLH LPVQAGSNKI LKKMNRKYTK EHYLEIIDKV RSNIPDIAIT TDIIVGFPGE TEEDFLETLD LVERVRFDAA YTFIYSKRAG TVAANMPDQV DDAVKHERLE RLIELQNKIS LEKSAELRGK IVEVLIEGIS KRDSNKLTSR TRTNKVVHFV GDESLIGKLA NVKITETKAW TMQGELVEVI R
Expression Region 1-471
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Thermoanaerobacter sp. (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB,Mammalian cell
Code CSB-MP534628TMJ
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MPTNIYVTEE ELKKQEKIMK EIAEENKGKN LYYHIETYGC QMNVHDSEKL AGMLEKMGYK YTENLEQADV LLFNTCAVRE HAEIRVLGRV SQMKELKARN PNLIIGVSGC MMQEKNVVEA IKEKYSYIDI VFGTHNIYKF PQLLWEALNS QDIVIDIIED TKNVIEELPV KRDSNLKAWV NIIYGCNNFC TYCIVPYTRG REKSRKPEDI IAEVKELAQK GYKEITLLGQ NVNSYGKDLD EDITFAKLLY KLNDIEGIER IRFMTSHPKD ISDELIYAMR DLDKVCEHLH LPVQAGSNKI LKKMNRKYTK EHYLEIIDKV RSNIPDIAIT TDIIVGFPGE TEEDFLETLD LVERVRFDAA YTFIYSKRAG TVAANMPDQV DDAVKHERLE RLIELQNKIS LEKSAELRGK IVEVLIEGIS KRDSNKLTSR TRTNKVVHFV GDESLIGKLA NVKITETKAW TMQGELVEVI R
Expression Region 1-471
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Still Have Questions? Leave a Message or Start an on-line Chat

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