Recombinant Xenopus laevis Forkhead box protein D3-B (foxd3-b)

Code CSB-YP887608XBE
MSDS
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Source Yeast
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Code CSB-EP887608XBE
MSDS
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Source E.coli
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Code CSB-EP887608XBE-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887608XBE
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP887608XBE
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
foxd3-b
Uniprot No.
Alternative Names
foxd3-b; foxd3bForkhead box protein D3-B; FoxD3-B; FoxD3b; Fork head domain-related protein 6'; xFD-6'
Species
Xenopus laevis (African clawed frog)
Expression Region
1-371
Target Protein Sequence
MTLSSSGSAS DMSGQTVLSA DDADIDVVGE GDEALDKDSE CESPVGHHDE VDALGGKEIP RSPSGSSTDA EGKGESQQQQ QEGIQNKPKN SVVKPPYSYI ALITMSILQS PQKKLTLSGI CEFISSRFPY YREKFPAWQN SIRHNLSLND CFIKIPREPG NPGKGNYWTL DPQSEDMFDN GSFLRRRKRF KRQQQDTLRE QTALMMQSFG AYSLASPYGR HYGLHPAAYT HPAALQYPYI PPVGPMLPPA VPLLPSSELT RKAFSSQLSP SLQLQLSSLS STAASIIKSE PSSRPSFSIE NIIGVSAASS VAPQTFLRPP VTVQSALMSH QPLVLSRSTA AIGPILSVPT NLISGQFLPT AATAVAKWPA Q
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional repressor that is an essential upstream regulator of neural crest determination and mesoderm induction. Functions by recruiting the transcriptional corepressor tle4. Also acts in a negative auto-regulator loop by inhibiting the transcription of its own gene.
Subcellular Location
Nucleus.
Tissue Specificity
Initially located within the dorsal blastopore lip (Spemann organizer) at the mid-gastrula stage. As expression fades in the neuroectoderm, expression begins in the head mesoderm in two regions at the lateral borders of the presumptive neural plate. These
Database Links

KEGG: xla:373610

UniGene: Xl.523

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