Recombinant Xenopus laevis Forkhead box protein I1-A (foxi1-a)

Code CSB-YP838637XBE
MSDS
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Source Yeast
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Code CSB-EP838637XBE
MSDS
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Source E.coli
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Code CSB-EP838637XBE-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP838637XBE
MSDS
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Source Baculovirus
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Code CSB-MP838637XBE
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
foxi1-a
Uniprot No.
Alternative Names
foxi1-aForkhead box protein I1-A; FoxI1-A; FoxI1a; xFoxI1a; Fork head domain-related protein 2; xFD-2; xFD2
Species
Xenopus laevis (African clawed frog)
Expression Region
1-370
Target Protein Sequence
MNPVQQPAQH KCPASSLNPP HPKRAQEAPD MGLYCDNFMY QQHNLHPSHR ATNFSIGDFT HQANPYLWLG GPGVNNSPSY SPTPAPYIPP AFSAPQRQFL ANSAAFGGAD LGWMSAASQE ELLKRVRPPY SYSALIAMSI QNATDKRLTL SQIYQYVAEN FPFYKKSKAG WQNSIRHNLS LNDCFKKMPR DENDPGKGNY WTLDSNCEKM FDNGNFRRKR KPKSETNNIK IAKREEDHVS PKGKESPPMI TPSSPKELSP TGHSKCPSPP TVTYTPCLTN FIGSMTAVDS ATMNRQGPLG LLNELSQRNL NGLSSFISGS AVDQSPEHQD SSLFYNRSPY YSSLPTSNQK QPPYLQQLHP QQSPLYQGRY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor. Essential for ventral specification of the early cephalic (head) ectoderm during gastrulation, playing a role in the non-neural versus neural cell fate choice. Binds to DNA via the target sequence 5'-[AG]TAAA[CT]A-3', with 5'-ATAAACA-3' being the preferred binding site.
Gene References into Functions
  1. Foxi1a is an essential regulator of ventral specification of the early head ectoderm during gastrulation PMID: 16079156
Subcellular Location
Nucleus.
Tissue Specificity
Initially localized to the animal hemisphere (the presumptive ectoderm) of early-mid blastula embryos. Becomes restricted to head placodes, excluding the otic placodes, by the tailbud stages.
Database Links

KEGG: xla:397954

UniGene: Xl.5369

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