Recombinant Xenopus laevis Homeobox protein otx5-A (otx5-a)

Code CSB-YP868709XBE
MSDS
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Source Yeast
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Code CSB-EP868709XBE
MSDS
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Source E.coli
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Code CSB-EP868709XBE-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP868709XBE
MSDS
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Source Baculovirus
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Code CSB-MP868709XBE
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
otx5-a
Uniprot No.
Alternative Names
otx5-a; Homeobox protein otx5-A; Orthodenticle homolog 5-A; Xotx5
Species
Xenopus laevis (African clawed frog)
Expression Region
1-290
Target Protein Sequence
MMSYIKQPHY AVNGLTLAGT GMDLLHSAVG YPTTPRKQRR ERTTFTRAQL DILESLFAKT RYPDIFMREE VALKINLPES RVQVWFKNRR AKCRQQQQQS TGQAKPRPAK KKTSPARETN SEASTNGQYS PPPPGTAVTP SSTAGATVSI WSPASISPIP DPLSIATTPC MQRSAGYPMT YSQAPAYTQS YGGSSSYFTG LDCGSYLSPM HPQLSAPGST LSPIASSTMG SHLSQSPASL SAQGYGASSL GFTSVDCLDY KDQTASWKLN FNATDCLDYK DQSSWKFQVL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor involved in anterior and eye development. Promotes the differentiation of both rod and cone photoreceptors cells in the retina. Together with other retinal homeobox proteins, acts as an effector of a cellular clock which, depending on cell cycle progression, establishes the generation of distinct retinal neuronal cell types. Acts synergistically with nrl to activate the rhodopsin promoter. Promotes the formation of anterior regions and represses the formation of posterior structures during development.
Gene References into Functions
  1. A small 'specificity box' is necessary to confer on XOTX2 and XOTX5b their distinct activities in the developing frog retina and to convert the neutral orthologous OTD protein of Drosophila into a positive and specific XOTX-like retinal regulator. PMID: 17597530
Subcellular Location
Nucleus.
Tissue Specificity
Initially expressed in the Spemann organizer region of early gastrula embryos. Expression then becomes localized to the anterior region; confined to the cement gland anlage at stages 19-25 and localized to the forebrain, optic vesicle and otocyst at stage
Database Links

KEGG: xla:373653

UniGene: Xl.42160

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