Recombinant Xenopus laevis Methylosome subunit pICln (clns1a)

Code CSB-YP005572XBE
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP005572XBE
MSDS
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Source E.coli
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Code CSB-EP005572XBE-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP005572XBE
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP005572XBE
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
clns1a
Uniprot No.
Alternative Names
clns1a; icln; Methylosome subunit pICln; Chloride conductance regulatory protein ICln; I(Cln)
Species
Xenopus laevis (African clawed frog)
Expression Region
1-241
Target Protein Sequence
MNLLSSFPPP ADGVRRLQPG TEAVVGGRGL GPGTLYIAES RLSWLNGSGL GFSLEYPSIS LHAISRDTAA YPEEHLYVMV NSKLADKEDK EAHMADQEEE ESEDDDDDEE PITEIRFVPG EKSDLGEMFS AMCDCQALHP DPEDADSDDD YEGDEYDVEA HEQGQVDVPT FYTYEEGLSH LTTEGQATLE RLENMLSNSI GNQHTMAGVR TEGPALEPED GMDVENTQTV AGQFEDADVD H
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in both the assembly of spliceosomal snRNPs and the methylation of Sm proteins. Chaperone that regulates the assembly of spliceosomal U1, U2, U4 and U5 small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus.
Subcellular Location
Cytoplasm, cytosol. Nucleus. Cytoplasm, cytoskeleton.
Protein Families
PICln (TC 1.A.47) family
Database Links

KEGG: xla:398038

UniGene: Xl.47036

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