Recombinant Xenopus laevis Probable E3 ubiquitin-protein ligase makorin-2 (mkrn2)

Code CSB-YP014606XBE
MSDS
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Source Yeast
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Code CSB-EP014606XBE
MSDS
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Source E.coli
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Code CSB-EP014606XBE-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP014606XBE
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP014606XBE
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mkrn2
Uniprot No.
Alternative Names
mkrn2Probable E3 ubiquitin-protein ligase makorin-2; EC 2.3.2.27; RING-type E3 ubiquitin transferase makorin-2
Species
Xenopus laevis (African clawed frog)
Expression Region
1-409
Target Protein Sequence
MSPKQVTCRY FLHGVCREGS RCLFSHDLAT SKPSTVCRFF LRGQCAYGTR CRYDHVKPCN GTVFIPPQEM SPVLSPPPLF PAQEAAVPPT IPAPQRREKK TLVLRDRDLC GASVDPALQP GCITESQGSE GEAKPHSYLE AICTGLDESQ DPASYPGAPQ QLCPFAQAGE CHCGDSCPYL HGDACEICGL QVLHPHDQEQ RRDHEKLCME NFELDMERAF AVQASEGRVC SICMERVYEK QSPAQRRFRI LSDCNHTYCL TCIRQWRCAR QFDNPVIKSC PECRVISEFV IPSAYWVEDQ SKKDELIEAF KQGMGKKLCK YFDQGRGTCP FGGKCLYLHS YPDGTRAQPE KPRKQLGSEG SVRFLNSLRL WDFIEDREQR GVPNAEVGKL GELFMHLSGA DEELPAPFN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Inhibits neurogenesis and axis formation during embryonic development by modulating the phosphatidylinositol 3-kinase (PI3K) pathway. Acts downstream of PI3K and akt1 to up-regulate gsk3b mRNA expression.
Gene References into Functions
  1. These results collectively suggest that the third C(3)H zinc finger, Cys-His motif and C(3)HC(4) RING zinc finger are indispensable for the anti-neurogenic activity of mkrn2. PMID: 20167204
  2. mkrn2 acts downstream of PI3K and Akt and upstream of GSK-3beta PMID: 18198183
Tissue Specificity
In adult males, highly expressed in testis, small intestine, large intestine, stomach, heart, liver, kidney, lung and muscle. Consistent with its role as a neurogenic inhibitor, expression is much lower in the brain, and also in skin.
Database Links

KEGG: xla:100141485

UniGene: Xl.76333

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