Recombinant Xenopus tropicalis Poly (ADP-ribose) glycohydrolase ARH3 (adprhl2)

Code CSB-YP637950XBF
MSDS
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Source Yeast
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Code CSB-EP637950XBF
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Source E.coli
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Code CSB-EP637950XBF-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP637950XBF
MSDS
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Source Baculovirus
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Code CSB-MP637950XBF
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
adprhl2
Uniprot No.
Alternative Names
adprhl2; arh3; TGas084j22.1ADP-ribose glycohydrolase ARH3; ADP-ribosylhydrolase 3; O-acetyl-ADP-ribose deacetylase ARH3; EC 3.5.1.-; Poly(ADP-ribose) glycohydrolase ARH3; EC 3.2.1.143; [Protein ADP-ribosylarginine] hydrolase-like protein 2; [Protein ADP-ribosylserine] hydrolase; EC 3.2.2.-
Species
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Expression Region
1-350
Target Protein Sequence
MMAAGVSRFR GSLLGALLGD CIGAVFEGHT NVTKEFLFDY MKSLDKGERL KRVLTYTDDT AMARSIVQSV LENYEFNIED LANRFTTEYN RDPDRGYGMA VVHVFEKLGS GEYKHVFSPA REQFDGKGSY GNGAAMRVVG ISLAYPRIPD IIEYARTSGM LTHASSLGYN GAILQALAVH YALQGELAPE TFLDQLLDHM KEVETDKKSR SDALELEMDE FPYCNKLRKI KAFLAREDVT RKDIVKELGN GIQAFESVPT AIYSFLRCLK PVSELPSELT NLQRTIAFCI LLGGDTDTIA TMAAAIAGAY HGEEQIPLNW KLSAEGYKDA EDWGEKLHQL YCRRLQSTTS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
ADP-ribose glycohydrolase that preferentially hydrolyzes the scissile alpha-O-linkage attached to the anomeric C1'' position of ADP-ribose and acts on different substrates, such as proteins ADP-ribosylated on serine, free poly(ADP-ribose) and O-acetyl-ADP-D-ribose. Specifically acts as a serine mono-ADP-ribosylhydrolase by mediating the removal of mono-ADP-ribose attached to serine residues on proteins, thereby playing a key role in DNA damage response. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Does not hydrolyze ADP-ribosyl-arginine, -cysteine, -diphthamide, or -asparagine bonds. Also able to degrade protein free poly(ADP-ribose), which is synthesized in response to DNA damage: free poly(ADP-ribose) acts as a potent cell death signal and its degradation by ADPRHL2 protects cells from poly(ADP-ribose)-dependent cell death, a process named parthanatos. Also hydrolyzes free poly(ADP-ribose) in mitochondria. Specifically digests O-acetyl-ADP-D-ribose, a product of deacetylation reactions catalyzed by sirtuins. Specifically degrades 1''-O-acetyl-ADP-D-ribose isomer, rather than 2''-O-acetyl-ADP-D-ribose or 3''-O-acetyl-ADP-D-ribose isomers.
Subcellular Location
Nucleus. Cytoplasm. Chromosome. Mitochondrion matrix.
Protein Families
ADP-ribosylglycohydrolase family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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