HEK293T/Human SARM1 Stable Cell Line

Code CSB-SC750971HU
Size 1 vial contains approximately 5x106 cells in 1 ml
Price $5000
  • Untransfected HEK293T cells (green line) and transfected Human SARM1 HEK293T Stable cells (red line) were stained with anti-Flag antibody (2µg/1*106cells), washed and then followed by FITC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry.
  • Western Blot
    Positive WB detected in: Lane1:20 µg HEK293T cells, Lane2:40 µg Human SARM1 HEK293T Stable cells, Lane3:20 µg Human SARM1 HEK293T Stable cells
    All lanes: Anti-Flag antibody at 1:1000
    Goat polyclonal to mouse IgG at 1/50000 dilution
    Predicted band size: 77.5 KDa
    Observed band size: 77 KDa
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Product Details

Uniprot No.
Growth Properties
Selection Marker
Culture Medium
DMEM + 10% FBS + 2μg/mL Puromycin
Alternative Names
Tag Info
C-terminal Flag-tagged
35% FBS + 55% DMEM + 10% DMSO
Tested Applications
Binding assay by FACS and Western blot
Frozen in liquid nitrogen or stored at -80°C
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

(From Uniprot)
NAD(+) hydrolase, which plays a key role in axonal degeneration following injury by regulating NAD(+) metabolism. Acts as a negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway by promoting Wallerian degeneration, an injury-induced form of programmed subcellular death which involves degeneration of an axon distal to the injury site. Wallerian degeneration is triggered by NAD(+) depletion: in response to injury, SARM1 is activated and catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR), cyclic ADPR (cADPR) and nicotinamide; NAD(+) cleavage promoting cytoskeletal degradation and axon destruction. Also able to hydrolyze NADP(+), but not other NAD(+)-related molecules. Can activate neuronal cell death in response to stress. Regulates dendritic arborization through the MAPK4-JNK pathway. Involved in innate immune response: inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38.
Gene References into Functions
  1. we identify a physical interaction between the autoinhibitory N terminus and the TIR domain of SARM1, revealing a previously unrecognized direct connection between these domains that we propose mediates autoinhibition and activation upon injury. PMID: 27671644
  2. Active nerve degeneration requires SARM1 and MAP kinases, including DLK, while the NAD+ synthetic enzyme NMNAT2 prevents degeneration. PMID: 26844829
  3. Data show that sterile alpha- and armadillo-motif-containing protein (SARM) modulates MyD88 protein-mediated Toll-like receptors (TLRs) activation through BB-loop dependent interleukin-1 receptor (TIR) TIR-TIR interactions. PMID: 26592460
  4. These results indicate that association of PINK1 with SARM1 and TRAF6 is an important step for mitophagy. PMID: 23885119
  5. The innate immunity adaptor SARM translocates to the nucleus to stabilize lamins and prevent DNA fragmentation in response to pro-apoptotic signaling. PMID: 23923041
  6. Rapid Wallerian degeneration requires the pro-degenerative molecules SARM1. PMID: 24840802
  7. Data found that the UXT isoforms elicit dual opposing regulatory effects on SARM-induced apoptosis. PMID: 24021647
  8. SARM overexpression caused mitochondrial clustering which has also been observed in several cell death phenomenon. PMID: 23175186
  9. The N-terminal 27 amino acids (S27) of SARM, which is hydrophobic and polybasic, acts as a mitochondria-targeting signal sequence, associating SARM to the mitochondria. The S27 peptide has an inherent ability to bind to lipids and mitochondria. PMID: 22145856
  10. SARM-mediated inhibition may not be exclusively directed at TRIF or MyD88, but that SARM may also directly inhibit MAPK phosphorylation PMID: 20306472
  11. Candidate gene in the onset of hereditary infectious/inflammatory diseases. PMID: 15893701
  12. TIR adaptor SARM is a negative regulator of Toll-like receptor signaling. PMID: 16964262
  13. confirmed the co-localization of retinoschisin with Na/K ATPase and SARM1 in photoreceptors and bipolar cells of retina tissue PMID: 17804407
  14. SARM1 deficiencies may uncover unexpected similarities between the ways in which neurons and immune cells sense and respond to danger. PMID: 18089857

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Subcellular Location
Cytoplasm. Cell projection, axon. Cell projection, dendrite. Cell junction, synapse. Mitochondrion.
Tissue Specificity
Predominantly expressed in brain, kidney and liver. Expressed at lower level in placenta.
Database Links

HGNC: 17074

OMIM: 607732

KEGG: hsa:23098

STRING: 9606.ENSP00000406738

UniGene: Hs.743510

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