Name: HEK293T/Human SARM1 Stable Cell Line
Brand: CUSABIO
SKU: CSB-SC750971HU

Product Details

Uniprot No.	Q6SZW1
Growth Properties	Adherent
Selection Marker	Puromycin
Culture Medium	DMEM + 10% FBS + 2μg/mL Puromycin
Alternative Names	SARM1
Tag Info	C-terminal Flag-tagged
Buffer	35% FBS + 55% DMEM + 10% DMSO
Tested Applications	Binding assay by FACS and Western blot
Storage	Frozen in liquid nitrogen or stored at -80°C
Lead Time	Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function (From Uniprot)	NAD(+) hydrolase, which plays a key role in axonal degeneration following injury by regulating NAD(+) metabolism. Acts as a negative regulator of MYD88- and TRIF-dependent toll-like receptor signaling pathway by promoting Wallerian degeneration, an injury-induced form of programmed subcellular death which involves degeneration of an axon distal to the injury site. Wallerian degeneration is triggered by NAD(+) depletion: in response to injury, SARM1 is activated and catalyzes cleavage of NAD(+) into ADP-D-ribose (ADPR), cyclic ADPR (cADPR) and nicotinamide; NAD(+) cleavage promoting cytoskeletal degradation and axon destruction. Also able to hydrolyze NADP(+), but not other NAD(+)-related molecules. Can activate neuronal cell death in response to stress. Regulates dendritic arborization through the MAPK4-JNK pathway. Involved in innate immune response: inhibits both TICAM1/TRIF- and MYD88-dependent activation of JUN/AP-1, TRIF-dependent activation of NF-kappa-B and IRF3, and the phosphorylation of MAPK14/p38.
Gene References into Functions	we identify a physical interaction between the autoinhibitory N terminus and the TIR domain of SARM1, revealing a previously unrecognized direct connection between these domains that we propose mediates autoinhibition and activation upon injury. PMID: 27671644 Active nerve degeneration requires SARM1 and MAP kinases, including DLK, while the NAD+ synthetic enzyme NMNAT2 prevents degeneration. PMID: 26844829 Data show that sterile alpha- and armadillo-motif-containing protein (SARM) modulates MyD88 protein-mediated Toll-like receptors (TLRs) activation through BB-loop dependent interleukin-1 receptor (TIR) TIR-TIR interactions. PMID: 26592460 These results indicate that association of PINK1 with SARM1 and TRAF6 is an important step for mitophagy. PMID: 23885119 The innate immunity adaptor SARM translocates to the nucleus to stabilize lamins and prevent DNA fragmentation in response to pro-apoptotic signaling. PMID: 23923041 Rapid Wallerian degeneration requires the pro-degenerative molecules SARM1. PMID: 24840802 Data found that the UXT isoforms elicit dual opposing regulatory effects on SARM-induced apoptosis. PMID: 24021647 SARM overexpression caused mitochondrial clustering which has also been observed in several cell death phenomenon. PMID: 23175186 The N-terminal 27 amino acids (S27) of SARM, which is hydrophobic and polybasic, acts as a mitochondria-targeting signal sequence, associating SARM to the mitochondria. The S27 peptide has an inherent ability to bind to lipids and mitochondria. PMID: 22145856 SARM-mediated inhibition may not be exclusively directed at TRIF or MyD88, but that SARM may also directly inhibit MAPK phosphorylation PMID: 20306472 Candidate gene in the onset of hereditary infectious/inflammatory diseases. PMID: 15893701 TIR adaptor SARM is a negative regulator of Toll-like receptor signaling. PMID: 16964262 confirmed the co-localization of retinoschisin with Na/K ATPase and SARM1 in photoreceptors and bipolar cells of retina tissue PMID: 17804407 SARM1 deficiencies may uncover unexpected similarities between the ways in which neurons and immune cells sense and respond to danger. PMID: 18089857 Show More Hide All
Subcellular Location	Cytoplasm. Cell projection, axon. Cell projection, dendrite. Cell junction, synapse. Mitochondrion.
Tissue Specificity	Predominantly expressed in brain, kidney and liver. Expressed at lower level in placenta.
Database Links	HGNC: 17074 OMIM: 607732 KEGG: hsa:23098 STRING: 9606.ENSP00000406738 UniGene: Hs.743510

CloseMessage

Product >> Product Name：
Product >> Code：
Product >> Size：
Name： *
Email： *
Phone：
We recommend you to leave your phone number because occasionally our emails get flagged by spam filters. We would not disclose any of your private information to any third party.
Question/Comment： *
Your Region： *
Country State：
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
Check Code：

Code	CSB-SC750971HU
Size	1 vial contains approximately 5x10⁶ cells in 1 ml
Price	$5000
Image	Untransfected HEK293T cells (green line) and transfected Human SARM1 HEK293T Stable cells (red line) were stained with anti-Flag antibody (2µg/1*10⁶cells), washed and then followed by FITC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry. Western Blot Positive WB detected in: Lane1：20 µg HEK293T cells, Lane2：40 µg Human SARM1 HEK293T Stable cells, Lane3：20 µg Human SARM1 HEK293T Stable cells All lanes: Anti-Flag antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 77.5 KDa Observed band size: 77 KDa
Have Questions?	Leave a Message or Start an on-line Chat

HEK293T/Human SARM1 Stable Cell Line

Product Details

Related Products

Customer Reviews and Q&A

Target Background

CloseMessage

×CloseMessage