Recombinant Mouse Bestrophin-3 (Best3)

Code CSB-CF760699MO
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
Best3
Uniprot No.
Alternative Names
Best3; Vmd2l3; Bestrophin-3; Vitelliform macular dystrophy 2-like protein 3
Species
Mus musculus (Mouse)
Expression Region
1-669
Target Protein Sequence
MTVTYSSKVANATFFGFHRLLLKWRGSIYKLLYREFIVFAVLYTAISLVYRLLLTGAQKR YFEKLSIYCDRYAEQIPVTFVLGFYVTLVVNRWWNQFVNLPWPDRLMLLISSSVHGSDQH GRLLRRTLMRYVNLTSLLIFRSVSTAVYKRFPTMDHVVEAGFMTADERKLFDHLKSPHLK YWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGIPLVYTQVV TLAVYTFFFACLIGRQFLDPTKGYVGHDLDLYVPIFTLLQFFFYAGWLKVAEQLINPFGE DDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLAAADYCIPSFL GSTIQMGLSGSNFPAEDWLWNYEKHGNRHSVMRRVKRFLSTHEHPGSPRRRRSFGRQASD SSMFLPPSPARDLLDVPSRNPHRGSPTRKQSRSQEGSPKLHSSMGELSTIRETSRTSTLQ SLSPQSSVRSSPTKMPQVPEVLITAAEAPAFSADSHQHDSTTSILSLEFTGVQPSGTEQQ VEPSGTPPGDPNPQTTSASTERDLFKFEEDLEDDRFPKRWSLPEFLESRHTSLGNLGPDP VSPRDALLLPDTETPSETNGIHPGAGSALAPDILYLMESLDKETDILEFNNEHTGESPKG TPQRPRTWF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Forms calcium-sensitive chloride channels. Permeable to bicarbonate.
Gene References into Functions
  1. The authors propose that Best3V2 isoform in myoblasts may work as a tuner to control Ca(2+) release from intracellular Ca(2+) stores. PMID: 27265833
  2. Our experiments present clear evidence of Best3 mRNA and protein expression in mouse kidney glomeruli PMID: 25912364
  3. results demonstrated that Best-3 is an endogenous inhibitor of NF-kappaB signaling pathway in endothelial cells, suggesting that forced Best-3 expression may be a novel approach for the treatment of vascular inflammatory diseases. PMID: 25329324
  4. The channel activity of the mBest4 protein lacking the C-terminus (residues 353-669) was specifically inhibited by co-expression of C-terminal fragments of mBest4 having the inhibitory motif. PMID: 16563389
  5. These results suggest that an auto-inhibitory mechanism in C termini of bestrophin 3 may be universal among bestrophins investigated in the study. PMID: 17442670

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
Bestrophin family
Database Links
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