Recombinant Mouse Claudin-2 (Cldn2)

Code CSB-CF005500MO
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
Cldn2
Uniprot No.
Alternative Names
Cldn2; Claudin-2
Species
Mus musculus (Mouse)
Expression Region
1-230
Target Protein Sequence
MASLGVQLVGYILGLLGLLGTSIAMLLPNWRTSSYVGASIVTAVGFSKGLWMECATHSTG ITQCDIYSTLLGLPADIQAAQAMMVTSSAMSSLACIISVVGMRCTVFCQDSRAKDRVAVV GGVFFILGGILGFIPVAWNLHGILRDFYSPLVPDSMKFEIGEALYLGIISALFSLVAGVI LCFSCSPQGNRTNYYDGYQAQPLATRSSPRSAQQPKAKSEFNSYSLTGYV
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Gene References into Functions
  1. results reveal that IL-22 increases intestinal epithelial permeability by upregulating Claudin-2 expression through the JAK/STAT pathway PMID: 28939759
  2. claudin-2-null mice conserve sodium to the same extent as WT mice, even during profound dietary sodium depletion, as a result of the upregulation of transcellular Na-K-2Cl transport activity in the thick ascending limb of Henle PMID: 27214555
  3. The tight junction patch clamp technique indicated mouse claudin-2 ion channel opening events in MDCK cells PMID: 28605031
  4. The current study reveals an important and novel mechanism for VDR by regulation of epithelial barriers. PMID: 26212084
  5. findings reveal a critical albeit complex role of claudin-2 in intestinal homeostasis by regulating epithelial permeability, inflammation and proliferation PMID: 24670427
  6. Based on studies of Cldn2(-/-) mice, claudin 2 regulates paracellular ion and water flow required for proper regulation of bile composition and flow. PMID: 25068494
  7. IL-6 modulation of intestinal TJ permeability was regulated by JNK activation of AP-1 and AP-1 activation of claudin-2 gene PMID: 24662742
  8. this study set out to map out all the pore-lining residues of claudin-2 by comprehensive cysteine-scanning mutagenesis of the entire first extracellular loop (ECL1). PMID: 24436330
  9. The capacity of non-AQP1-dependent transcellular proximal fluid reabsorption (PFR) is sufficient to be maintained in AQP1- and claudin-2-doubly deficient knockout mice. PMID: 24049145
  10. These results suggest that the ERK pathway positively regulates claudin-2 protein expression and claudin-2 is involved in lowering TER in CMT93-II cells. PMID: 23369528
  11. Claudin-2 assumes an important role in colorectal inflammation, and furthermore implicates the involvement of MLCK in colon inflammation. PMID: 23306855
  12. cingulin is dispensable for the structure and barrier function of tight junctions, and is embedded in signaling networks that control the expression of claudin-2. PMID: 22946046
  13. Together these data are consistent with phosphorylation at S208 playing a major role in the retention of claudin-2 at the plasma membrane PMID: 22825868
  14. STAT6 regulates claudin-2 induction in oxazolone (OXA) colitis; claudin-2 staining is increased in wild-type OXA mice but not in STAT6-deficient animals. PMID: 23303670
  15. Claudin-2 is expressed at the variety of epithelial tissues in the coclea of inner ear including Organ of Corti, stria vascularis, Reissner's membrane, spiral limbus. PMID: 14698084
  16. during breast cancer liver metastasis, claudin-2 shifts from acting within tight-junctional complexes to functioning as an adhesion molecule between breast cancer cells and hepatocytes. PMID: 22645303
  17. Claudin-2 expression was abnormal at the plasma membrane of intestinal epithelial cells in adherent-invasive Escherichia coli-infected CEABAC10 mice. PMID: 21688348
  18. Claudin-2 promoter activity is increased by IL-6 in a MEK/ERK and PI3K-dependent manner PMID: 21771795
  19. Claudin 2 is indispensable for paracellular sodium permeability in the small intestine. PMID: 20727355
  20. Claudin-2 was expressed in the base of the glands in intestine and intestinal metaplasia while it was not expressed in the body of stomach. PMID: 20602571
  21. BN-PAGE of mouse liver membrane identified a relatively discrete tight junction structure of a high molecular weight complex containing at least claudin-1, claudin-2, and occludin PMID: 21098027
  22. claudin-2 constitutes leaky and cation (Na(+))-selective paracellular channels within tight junctions of mouse proximal tubules. PMID: 20385797
  23. Results support a model in which claudins 2 and 4 create paracellular channels and the first extracellular domain is sufficient to determine both paracellular charge selectivity and transepithelial electrical resistance. PMID: 12700140
  24. The tail of claudin-2 could stabilize claudin-4, with a concomitant increase in both protein level and physiologic influence. PMID: 15366421
  25. the ERK 1/2 signaling pathway negatively controls claudin-2 expression in mammalian renal epithelial cells and provide evidence for regulation of tight junction paracellular transport by alterations in claudin composition within tight junction complexes. PMID: 15569684
  26. These findings strongly suggest that claudin-2- and/or claudin-12-based tight junctions form paracellular Ca(2+) channels in intestinal epithelia, and they highlight a novel mechanism behind vitamin D-dependent calcium homeostasis. PMID: 18287530
  27. Molecular basis for cation selectivity in claudin-2-based pores PMID: 19538287

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Subcellular Location
Cell junction, tight junction. Cell membrane; Multi-pass membrane protein.
Protein Families
Claudin family
Tissue Specificity
Expressed in the kidney, liver and intestine, with higher levels in the ileum than in the jejunum. Low levels in the brain.
Database Links
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