Recombinant Rat Amine oxidase [flavin-containing] B (Maob)

Code CSB-CF013399RA
MSDS
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Source in vitro E.coli expression system
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Product Details

Target Names
Maob
Uniprot No.
Alternative Names
Maob; Amine oxidase [flavin-containing] B; Monoamine oxidase type B; MAO-B
Species
Rattus norvegicus (Rat)
Expression Region
2-520
Target Protein Sequence
SNKCDVIVVGGGISGMAAAKLLHDCGLSVVVLEARDRVGGRTYTIRNKNVKYVDLGGSYVGPTQNRILRLAKELGLETYKVNEVERLIHFVKGKSYAFRGPFPPVWNPITYLDYNNLWRTMDEMGQEIPSDAPWKAPLAEEWDYMTMKELLDKICWTNSTKQIATLFVNLCVTAETHEVSALWFLWYVKQCGGTTRIISTTNGGQERKFIGGSGQVSERIKDILGDRVKLERPVIHIDQTGENVVVKTLNHEIYEAKYVISAIPPVLGMKIHHSPPLPILRNQLITRVPLGSVIKCMVYYKEPFWRKKDFCGTMVIEGEEAPIAYTLDDTKPDGSCAAIMGFILAHKARKLVRLTKEERLRKLCELYAKVLNSQEALQPVHYEEKNWCEEQYSGGCYTAYFPPGILTQYGRVLRQPVGKIFFAGTETASHWSGYMEGAVEAGERAAREILHAIGKIPEDEIWQPEPESVDVPARPITNTFLERHLPSVPGLLKLLGLTTILSATALGFLAHKKGLFVRF
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine.
Gene References into Functions
  1. Benzylamine and serotonin oxidation were catalyzed by MAO-B and MAO-A, respectively, to aldehydes. These were derivatized with DNPH, and the corresponding quinones were further formed by adding NaOH. These DNPH derivatives with large conjugated structures were directly measured spectrophotometrically at 465 nm and 425 nm PMID: 27503749
  2. Monoamine oxidase B is increased in prefrontal cortex following ethanol administration. PMID: 20204567
  3. investigation of topological orientation of MAO-B (and MAO-A) in liver mitochondrial membranes PMID: 21341713
  4. Dopamine induces Ca2+ signaling in astrocytes through reactive oxygen species generated by monoamine oxidase PMID: 20547771
  5. significant effects of hormone replacement and gonadectomy on catechol-O-methyltransferase and monoamine oxidase isoforms in both striatum and cortex PMID: 19909795
  6. in serotonergic neurons only mitochondria lacking monoamine oxidase type B are transported by axonal flow up to axon terminals. PMID: 12379239
  7. MAO-B is localized to the outer membranes of mitochondria in nerve terminals of afferent and efferent fibers, as well as in taste bud cells. PMID: 14697889
  8. MAOB is involved in the inactivation mechanism of histamine that is released from ECL cells and activates parietal cells to secrete gastric acid. PMID: 14986002
  9. monoamine oxidase activity has a role in regulating reactive oxygen species production and aluminum-induced mitochondrial permeability transition PMID: 15526171
  10. Presence of monoamine oxidase type B protein but absence of associated enzyme activity in neurons within the inferior olive nucleus of the rat. PMID: 16098487
  11. These data suggest that the structural properties of the active site cavities in rat MAOs are significantly different from the two human enzymes, which correlates with the differences in the inhibitor specificities between human and rat MAOs. PMID: 18092818
  12. localization of monoamine oxidase type B in liver; no MAOB was found in endothelial cells, hepatic stellate cells, or Kupffer cells; MAOB was localized to the mitochondrial outer membrane of hepatocytes PMID: 18502718
  13. These results suggest that LPS induces chronic wounds via elevated MAO/B-mediated increases in H(2)O(2) and TNF-alpha activity by epithelial cells and is further associated with more distant effects on systemic oxidative stress and alveolar bone loss. PMID: 19779138

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Subcellular Location
Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side.
Protein Families
Flavin monoamine oxidase family
Database Links
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