All
  • All
  • ELISA Kit
  • Protein
  • Antibody
  • Molecular Biology Product
  • Small Molecule
All
  • All
  • ELISA Kit
  • Protein
  • Antibody
  • Molecular Biology Product
  • Small Molecule

Human Thrombospondin type-1 domain-containing protein 4(THSD4) ELISA kit

Protocol MSDS
Code CSB-EL023518HU
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
thrombospondin, type I, domain containing 4
Alternative Names
THSD4 ELISA Kit; UNQ9334/PRO34005 ELISA Kit; Thrombospondin type-1 domain-containing protein 4 ELISA Kit; A disintegrin and metalloproteinase with thrombospondin motifs-like protein 6 ELISA Kit; ADAMTS-like protein 6 ELISA Kit; ADAMTSL-6 ELISA Kit
Abbreviation
THSD4
Uniprot No.
Q6ZMP0
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
12.5 pg/mL-800 pg/mL
Sensitivity
3.12 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human THSD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:20 Average % 88  
Range % 81-95  
1:40 Average % 91  
Range % 86-97  
1:80 Average % 97  
Range % 91-102  
1:160 Average % 102  
Range % 97-107  
Recovery
The recovery of human THSD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 103 95-110  
EDTA plasma (n=4) 93 85-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
800 2.675 2.870 2.773 2.674  
400 2.324 2.345 2.335 2.236  
200 1.618 1.655 1.637 1.538  
100 0.864 0.901 0.883 0.784  
50 0.502 0.496 0.499 0.400  
25 0.324 0.345 0.335 0.236  
12.5 0.198 0.200 0.199 0.100  
0 0.101 0.096 0.099 　  
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human THSD4 ELISA Kit was designed for the quantitative measurement of Human THSD4 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.

Related Products

THSD4 Proteins

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Promotes FBN1 matrix assembly. Attenuates TGFB signaling, possibly by accelerating the sequestration of large latent complexes of TGFB or active TGFB by FBN1 microfibril assembly, thereby negatively regulating the expression of TGFB regulatory targets, such as POSTN.
Gene References into Functions
  1. In breast cancer, some GATA3 effects shift from tumor suppressing to tumor promoting during tumorigenesis, with deregulation of three genes, BCL2, DACH1, THSD4, representing major GATA3-controlled processes in cancer progression. PMID: 25410484
  2. ADAMTSL6beta has a role in fibrillin-1 microfibril formation PMID: 21880733
  3. THSD4 polymorphism was not found to be associated with COPD risk PMID: 21965014
  4. Variations may be important determinants of osteoporosis in Japanese women. PMID: 18488137
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Database Links

HGNC: 25835

OMIM: 614476

KEGG: hsa:79875

STRING: 9606.ENSP00000261862

UniGene: Hs.387057

Promotions
Obtain Rewards by Referring A Friend
Reward For Reviews
Transmembrane Protein Questionnaire with Gifts
Free antibody trial
Contact Us

  Email: [email protected]
  Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Related Articles
ELISA Data Analysis How to Do The Pre-experiment before Your ELISA Experiment Four Types of ELISA 11 tips for choosing your right ELISA kit Do you Often Trouble in These Problems of ELISA? Problems and Solutions in ELISA Experiments
CUSABIO guaranteed quality
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of email
Email
[email protected]
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
Privacy Policy