Home
Kit
ELISA Kit
Rat Netrin-1(NTN1) ELISA kit

Rat Netrin-1(NTN1) ELISA kit

Citations (3) Protocol MSDS

Code	CSB-EL016127RA
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details
Citations (3)
Related Products
Customer Reviews and Q&A
Target Background

Product Details

Target Name

netrin 1

Alternative Names

Ntn1 ELISA Kit; Netrin-1 ELISA Kit

Abbreviation

NTN1

Uniprot No.

Q924Z9

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

18.75 pg/mL-1200 pg/mL

Sensitivity

4.7 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Neuroscience

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse LIPE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	93
	Range %	90-95
1:4	Average %	83
	Range %	80-86
1:8	Average %	110
	Range %	104-116

Recovery

The recovery of mouse LIPE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-97
EDTA plasma (n=4)	101	98-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

U/ml	OD1	OD2	Average	Corrected
10	2.115	2.201	2.158	2.025
5	1.658	1.638	1.648	1.515
2.5	1.182	1.201	1.192	1.059
1.25	0.745	0.738	0.742	0.609
0.625	0.418	0.431	0.425	0.292
0.312	0.325	0.319	0.322	0.189
0.156	0.204	0.211	0.208	0.075
0	0.134	0.132	0.133

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Rat NTN1 ELISA Kit was designed for the quantitative measurement of Rat NTN1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 18.75 pg/mL-1200 pg/mL and the sensitivity is 4.7 pg/mL.

Citations

Macrophage-derived netrin-1 is critical for neuroangiogenesis in endometriosis Guo X,Int J Biol Macromol,2020
Netrin-1 plays a role in the effect of moderate exercise on myocardial fibrosis in rats Daliang Z, et al,Plos One,2019
Netrin-1 plays a role in the effect of 10 weeks moderate exercise on myocardial fibrosis in rats. View ORCID Profilezhou daliang.et al,/,2018

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)

Netrins control guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. Binding to UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion. Involved in dorsal root ganglion axon projection towards the spinal cord. It also serves as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Involved in colorectal tumorigenesis by regulating apoptosis.

Subcellular Location

Secreted. Cytoplasm.

Database Links

STRING: 10116.ENSRNOP00000005255

UniGene: Rn.17851

Promotions

Transmembrane Protein Questionnaire with Gifts

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

ELISA Data Analysis How to Do The Pre-experiment before Your ELISA Experiment Four Types of ELISA 11 tips for choosing your right ELISA kit Do you Often Trouble in These Problems of ELISA? Problems and Solutions in ELISA Experiments

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Rat Netrin-1(NTN1) ELISA kit

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background