Rat Ubiquitin carboxyl-terminal hydrolase isozyme L1(UCHL1) ELISA kit

Code CSB-EL025541RA
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
Alternative Names
Uchl1 ELISA Kit; Ubiquitin carboxyl-terminal hydrolase isozyme L1 ELISA Kit; UCH-L1 ELISA Kit; EC 3.4.19.12 ELISA Kit; Neuron cytoplasmic protein 9.5 ELISA Kit; PGP 9.5 ELISA Kit; PGP9.5 ELISA Kit; Ubiquitin thioesterase L1 ELISA Kit
Abbreviation
UCHL1
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
0.78 ng/mL-50 ng/mL
Sensitivity
0.195 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat UCHL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %89
Range %84-93
1:2Average %101
Range %98-106
1:4Average %89
Range %85-93
1:8Average %104
Range %100-108
Recovery
The recovery of rat UCHL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8580-90
EDTA plasma (n=4)9693-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
502.196 2.174 2.185 2.050
251.618 1.635 1.627 1.492
12.51.071 1.098 1.085 0.950
6.250.682 0.654 0.668 0.533
3.120.450 0.436 0.443 0.308
1.560.356 0.368 0.362 0.227
0.780.251 0.265 0.258 0.123
00.134 0.136 0.135
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The Rat Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) ELISA Kit, a highly sensitive and specific assay for the quantitative detection of UCHL1 in serum, plasma, tissue homogenates, and cell lysates from the Rat species.

UCHL1,plays an important role in ubiquitin-proteasome system, is a protein that plays a crucial role in the regulation of cellular processes such as protein degradation and cell signaling. Dysregulation of UCHL1 has been implicated in various diseases, including Parkinson's disease and cancer. Our UCHL1 ELISA Kit offers a reliable and efficient means of measuring UCHL1 levels in biological samples, with a detection range of 0.78 ng/mL to 50 ng/mL and a sensitivity of 0.195 ng/mL.

The assay principle of our UCHL1 ELISA Kit is based on the sandwich method. During the assay process, UCHL1 in the sample is sandwiched between two UCHL1-specific antibodies, which are coated on the plate. The bound UCHL1 is then detected using a detection antibody conjugated with HRP and a chromogenic substrate, producing a colorimetric signal that is directly proportional to the UCHL1 concentration.

This UCHL1 ELISA Kit offers a quick assay time of 1-5 hours, with a sample volume of 50-100ul and a detection wavelength of 450 nm. It is suitable for use in cell biology research and has been optimized for use with Rat samples. Its quantitative assay principle, coupled with its wide detection range and high sensitivity, make it an invaluable tool for researchers studying the physiological and pathological roles of UCHL1 in cellular processes.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
(From Uniprot)
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Gene References into Functions
  1. UCH-L1 can inhibit TNF-alpha-induced vascular smooth muscle cell migration, and this kind of effect may be partially due to its suppression role in NF-kappaB activation. PMID: 30305579
  2. investigated temporal profile of astrocytic and neuronal injury markers after TBI; different mechanisms underlie clearance of UCH-L1 and GFAP in CSF and serum PMID: 25763798
  3. Potential of UCHL1 as biomarker for destruction of pancreatic beta cells. PMID: 25638021
  4. UCH-L1 is differently processed in neurons compared with clonal cell lines and that farnesylation does not account for the membrane association in neurons. PMID: 25326379
  5. The proteases HtrA2/Omi and UCH-L1 regulate TNF-induced necroptosis. PMID: 24090154
  6. in AD, Abeta may down-regulate UCH-L1 in the AD brain, which in turn impairs BDNF/TrkB-mediated retrograde signaling, compromising synaptic plasticity and neuronal survival. PMID: 23599427
  7. Restoring UCH-L1 activity by transduction of primary neurons with a UCH-L1 fusion protein protected neurons, while inhibition of UCH-L1 exacerbates hypoxic injury. PMID: 20933087
  8. PGP9.5 messenger was decreased in nitrofen-exposed animals on E18 (esophagus) or E15 (small bowel), and increased on E21 in the esophagus and E18 in small bowel. PMID: 21069350
  9. Zinc deficiency could induce downregulation of Uch-L1 expression. PMID: 19267071
  10. analysis of a possible mechanistic link between UCH-L1 modification by cyclopentenone prostaglandins and the etiology of neurodegeneration PMID: 20231490
  11. Complete loss of UCH-L1 immunoreactivity in orofacial skin nerve fibers in dithranol-treated animals. PMID: 17011532
  12. UCH-L1 is widely expressed in neurons in all brain regions analyzed, including dopamine neurons of the midbrain. In the periphery, UCH-L1 expression remains restricted to neurons in all organs examined. PMID: 17599367
  13. UCHL1 may partially attenuate vascular remodeling through inhibition of NF-kappaB activity. PMID: 17690318
  14. results demonstrated that zinc modulated the expression of ubiquitin C-terminal hydrolase L1 (Uch-L1) and cAMP-responsive element-binding protein (CREB) at the protein and/or transcription levels in hippocampal neurons PMID: 18616865

Show More

Hide All

Subcellular Location
Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor.
Protein Families
Peptidase C12 family
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X