ABF1 Antibody

Code CSB-PA321206XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ABF1 Polyclonal antibody
Uniprot No.
Target Names
ABF1
Alternative Names
ABF1 antibody; BAF1 antibody; OBF1 antibody; REB2 antibody; SBF1 antibody; YKL112W antibody; YKL505ARS-binding factor 1 antibody; Bidirectionally acting factor 1 antibody; DNA replication enhancer-binding protein OBF1 antibody; SFB-B antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ABF1 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein A/G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
General regulatory factor (GRF) that contributes to transcriptional activation of a large number of genes, as well as to DNA replication, silencing and telomere structure. Involved in the transcription activation of a subset of ribosomal protein genes. Binds the ARS-elements found in many promoters. Binds to the sequence 5'-TCN(7)ACG-3'. Influences on genome-wide nucleosome occupancy and affects chromatin structure, and probably dynamics. As a component of the global genome repair (GGR) complex, promotes global genome nucleotide excision repair (GG-NER) which removes DNA damage from nontranscribing DNA. Component of the regulatory network controlling mitotic and meiotic cell cycle progression.
Gene References into Functions
  1. We present evidence that Abf1 recruitment upon nutritional stress, also observed for representative ribosome biogenesis genes, favours RPG transcriptional rescue upon nutrient replenishment, thus pointing to nutrient-regulated Abf1 dynamics at promoters as a novel mechanism in ribosome biogenesis control. PMID: 27016735
  2. Results show that Abf1 contributes to local regions of chromatin structure by acting at both strong and weak binding sites, at proximal promoter regions and at sites farther upstream, over a very large fraction of the yeast genome. PMID: 21081559
  3. Results compare the ability of RAP1 and ABF1 to create a region of open chromatin near and to contribute to activated transcription at promoters. PMID: 15456886
  4. DNA sequences were found that are bound by ABF1 with nearly two-magnitude higher affinity as compared to the hitherto accepted ABF1 consensus sequence. PMID: 16083878
  5. The effect on global gene expression of loss of binding of Abf1. PMID: 17158163
  6. The present study revealed numerous loci previously unknown to be under Abf1 control, and it yielded evidence for the protein's variable DNA binding pattern during mitotic growth and meiotic development. PMID: 18305101
  7. Data suggest an involvement of Abf1p transcription factor in intergenomic signaling. PMID: 18977319
  8. Mutation of the I silencer ABF1-binding site at the HMLalpha locus caused loss of ABF1 binding, which resulted in a domain of reduced global genome nucleotide excision repair efficiency on one side of the ABF1-binding site PMID: 18996839

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Subcellular Location
Nucleus.
Protein Families
BAF1 family
Database Links

KEGG: sce:YKL112W

STRING: 4932.YKL112W

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