RFA1 Antibody

Code CSB-PA322138XA01SVG
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Product Details

Full Product Name Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RFA1 Polyclonal antibody
Uniprot No. P22336
Target Names RFA1
Alternative Names RFA1 antibody; BUF2 antibody; RPA1 antibody; YAR007C antibody; FUN3 antibody; Replication factor A protein 1 antibody; RF-A protein 1 antibody; DNA-binding protein BUF2 antibody; Replication protein A 69 kDa DNA-binding subunit antibody; Single-stranded DNA-binding protein antibody
Raised in Rabbit
Species Reactivity Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RFA1 protein
Immunogen Species Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables ① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time Made-to-order (14-16 weeks)

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Target Background

(From Uniprot)
As part of the replication protein A (RPA/RP-A), a single-stranded DNA-binding heterotrimeric complex, may play an essential role in DNA replication, recombination and repair. Binds and stabilizes single-stranded DNA intermediates, preventing complementary DNA reannealing and recruiting different proteins involved in DNA metabolism. Binds to single-stranded sequences participating in DNA replication in addition to those mediating transcriptional repression (URS1) and activation (CAR1). Stimulates the activity of a cognate strand exchange protein (SEP1). It cooperates with T-AG and DNA topoisomerase I to unwind template DNA containing the simian virus 40 origin of DNA replication.
Gene References into Functions
  1. Overexpression of Rad51 was also found to suppress the rfa1 BIR defects. We suggest that Rad51 binding to the ssDNA formed by excessive end resection and during D-loop migration can partially compensate for dysfunctional RPA. PMID: 28009302
  2. RPA-dependent recruitment of Mec1-Ddc2 is crucial for maintaining its homodimeric state at ssDNA and that Ddc2's recruitment domain and CCD are important for Mec1-dependent survival of UV-light-induced DNA damage. PMID: 29033322
  3. Force regulated dynamics of RPA1 on a DNA fork during DNA replication has been reported. PMID: 27016742
  4. secondary structures within single-stranded DNA are potent instigators of genome instability, and RPA and Mre11-Sae2 play important roles in preventing their formation and propagation, respectively. PMID: 26545079
  5. RPA could exclude Ku from a partially resected structure with a 22-nt ssDNA tail and thus restored processing by Exo1. However, at a 40-nt tail, Ku remained stably associated at the ssDNA-dsDNA junction, and RPA simultaneously engaged the ssDNA region. PMID: 26067273
  6. DNA break induction, the budding yeast SUMO ligase Siz2 collaborates with the ssDNA-binding complex RPA (replication protein A) to induce the sumoylation of recombination factors and confer damage resistance PMID: 26253534
  7. the N-terminal acidic region of Sgs1 interacts with Rpa70 and recruits Rad53 kinase to stalled forks PMID: 22820947
  8. Data indicate that replication protein A (RPA) complex RFA1 is required for DNA end resection by Sgs1-Dna2 and Exo1. PMID: 23706822
  9. A novel pathway of telomere end protection that utilizes a previously unsuspected function of RPA at the telomeres. PMID: 23312805
  10. The interaction of RPA with telomerase appears to be mediated by both yKu and the telomerase subunit Est1. PMID: 22354040
  11. this work has important implications for understanding the checkpoint and RPA-dependent DNA-damage responses in eukaryotic cells. PMID: 22194703
  12. Isw2 and Ino80 are both enriched at stalled replication forks and physically and specifically interact with a single-stranded DNA binding protein, replication protein A. PMID: 21930788
  13. Data show that Pif1 overexpression causes relocalization of the DNA damage response factors Rfa1 and Mre11 into nuclear foci and activation of the Rad53 DNA damage checkpoint kinase, indicating that the toxicity is caused by accumulation of DNA damage. PMID: 19704012
  14. RPA appears to play a role in strand invasion as well as in facilitating Rad51 binding to ssDNA, possibly by stabilizing the displaced ssDNA PMID: 14737196
  15. Structure of the DNA-binding domain of the large subunit of replication protein A, a single-stranded DNA (ssDNA)-binding heterotrimeric protein with multiple roles in DNA metabolism. Study of the domain's interactions with ssDNA & SV40 T antigen. [RPA70] PMID: 16043636
  16. These results support the model that the FACT family has an essential role in constructing nucleosomes during DNA replication, and suggest that RPA contributes to this process. PMID: 16678108
  17. Control of telomeric recombination by RFA1. PMID: 17202155
  18. We show that Pol eta is less accurate than other yeast DNA polym and the accessory proteins have little effect on fidelity when bypassing a TT dimer. Accessory proteins participate in pol eta functions, but they do not suppress UV mutagenesis. PMID: 17608453
  19. Rad52 displaces RPA from single-stranded DNA using the acidic domain as a molecular lever PMID: 18703507
  20. Results show that DNA damage response at eroded telomeres is characterized by the recruitment of Cdc13, replication protein A, DNA damage checkpoint proteins and the DNA repair protein Rad52 into a single focus. PMID: 19597487

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Subcellular Location Nucleus.
Protein Families Replication factor A protein 1 family
Database Links

KEGG: sce:YAR007C

STRING: 4932.YAR007C

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