Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RIF1 Polyclonal antibody

Uniprot No.

P29539

Target Names

RIF1

Alternative Names

RIF1 antibody; YBR275C antibody; YBR1743 antibody; Telomere length regulator protein RIF1 antibody; RAP1-interacting factor 1 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RIF1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Negatively regulates telomere length by preventing telomere elongation or promoting degradation of the telomere ends. Recruited to telomeres by interaction with the C-terminus of RAP1, which binds directly to telomeric repeat DNA. This may create a negative feedback loop in which the addition of new telomere repeats creates binding sites for inhibitors of telomere length extension. May also influence the balance of transcriptional silencing at telomeres and the silent mating type locus HMR, which is mediated by SIR (Silent Information Regulator) proteins including SIR3 and SIR4. RIF1 competes with SIR proteins for binding to the C-terminus of RAP1. In the absence of RIF1, a limiting cellular pool of SIR proteins may preferentially associate with RAP1 at sub-telomeric loci, causing enhanced telomeric silencing and attenuated silencing of the HMR locus.

Gene References into Functions

Rif1 SQ/TQ Cluster Domain (SCD) can modulate Rif1 function. As all Rif1 orthologs have one or more SCD domains, these results for yeast Rif1 have implications for the regulation of Rif1 function in humans and other organisms. PMID: 29544213
Engagement of the Rif1 N-terminal domain with telomeres proved essential for checkpoint control and telomere length regulation. Unexpectedly, Rif1 N-terminal domain also promoted NHEJ repair at DNA breaks in yeast, revealing a conserved role of Rif1 in DNA repair. PMID: 28604726
These data suggest that the repression of origin activation by Rif1-Glc7 is important to avoid the deleterious accumulation of stalled replication forks at the rDNA RFB, which become lethal when fork stability is compromised. Finally, we show that Rif1-Glc7, unlike Sir2, has an important effect on origin firing outside of the rDNA locus that serves to prevent activation of the DNA replication checkpoint PMID: 27820830
Rif1 is phosphorylated by Tel1 during early replication.Tel1 is required for phosphorylation of Rif1 Serine-1308. PMID: 25329891
Data link Rif1 to negative regulation of replication origin firing through recruitment of the Glc7 phosphatase. PMID: 24685139
Findings elucidate the mechanism of action of Rif1 in the control of DNA replication and demonstrate a role of PP1 phosphatases in the regulation of origin firing. PMID: 24656819
Rif1 mechanism regulates origin timing irrespective of proximity to a chromosome end. PMID: 24879017
Saccharomyces cerevisiae Rif1 cooperates with MRX-Sae2 in promoting DNA-end resection. PMID: 24692507
Rif1 (Rap1-interacting factor 1) controls DNA replication genome-wide and describe how Rif1 opposes DDK function by directing Protein Phosphatase 1 (PP1)-mediated dephosphorylation of the MCM complex PMID: 24532715
Rif1 has important physiological roles in preventing a cell cycle arrest to incipient or small single stranded DNA lesions occurring on chromosomes, particularly on chromosome ends. PMID: 22194703
Saccharomyces cerevisiae Rif1 is an important telomeric factor with an anti-checkpoint role. PMID: 22194704
data supported a model in which Pfa4-dependent palmitoylation of Rif1 anchored it to the inner nuclear membrane, influencing its role in heterochromatin dynamics PMID: 21844336

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Subcellular Location

Nucleus. Chromosome, telomere. Note=Localizes to telomeres. Telomere association begins in G1/S, attains a peak during late G2/S phase of the cell cycle, and is lost during telophase. Localization to telomeres may be increased by telomere uncapping caused by expression of a mutant telomerase RNA subunit (TLC1).

Protein Families

RIF1 family

Database Links

KEGG: sce:YBR275C

STRING: 4932.YBR275C

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RIF1 Antibody

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