RIF1 Antibody

Code CSB-PA333457XA01SVG
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Product Details

Full Product Name Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RIF1 Polyclonal antibody
Uniprot No. P29539
Target Names RIF1
Alternative Names RIF1 antibody; YBR275C antibody; YBR1743 antibody; Telomere length regulator protein RIF1 antibody; RAP1-interacting factor 1 antibody
Raised in Rabbit
Species Reactivity Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RIF1 protein
Immunogen Species Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables ① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time Made-to-order (14-16 weeks)

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Target Background

(From Uniprot)
Negatively regulates telomere length by preventing telomere elongation or promoting degradation of the telomere ends. Recruited to telomeres by interaction with the C-terminus of RAP1, which binds directly to telomeric repeat DNA. This may create a negative feedback loop in which the addition of new telomere repeats creates binding sites for inhibitors of telomere length extension. May also influence the balance of transcriptional silencing at telomeres and the silent mating type locus HMR, which is mediated by SIR (Silent Information Regulator) proteins including SIR3 and SIR4. RIF1 competes with SIR proteins for binding to the C-terminus of RAP1. In the absence of RIF1, a limiting cellular pool of SIR proteins may preferentially associate with RAP1 at sub-telomeric loci, causing enhanced telomeric silencing and attenuated silencing of the HMR locus.
Gene References into Functions
  1. Rif1 SQ/TQ Cluster Domain (SCD) can modulate Rif1 function. As all Rif1 orthologs have one or more SCD domains, these results for yeast Rif1 have implications for the regulation of Rif1 function in humans and other organisms. PMID: 29544213
  2. Engagement of the Rif1 N-terminal domain with telomeres proved essential for checkpoint control and telomere length regulation. Unexpectedly, Rif1 N-terminal domain also promoted NHEJ repair at DNA breaks in yeast, revealing a conserved role of Rif1 in DNA repair. PMID: 28604726
  3. These data suggest that the repression of origin activation by Rif1-Glc7 is important to avoid the deleterious accumulation of stalled replication forks at the rDNA RFB, which become lethal when fork stability is compromised. Finally, we show that Rif1-Glc7, unlike Sir2, has an important effect on origin firing outside of the rDNA locus that serves to prevent activation of the DNA replication checkpoint PMID: 27820830
  4. Rif1 is phosphorylated by Tel1 during early replication.Tel1 is required for phosphorylation of Rif1 Serine-1308. PMID: 25329891
  5. Data link Rif1 to negative regulation of replication origin firing through recruitment of the Glc7 phosphatase. PMID: 24685139
  6. Findings elucidate the mechanism of action of Rif1 in the control of DNA replication and demonstrate a role of PP1 phosphatases in the regulation of origin firing. PMID: 24656819
  7. Rif1 mechanism regulates origin timing irrespective of proximity to a chromosome end. PMID: 24879017
  8. Saccharomyces cerevisiae Rif1 cooperates with MRX-Sae2 in promoting DNA-end resection. PMID: 24692507
  9. Rif1 (Rap1-interacting factor 1) controls DNA replication genome-wide and describe how Rif1 opposes DDK function by directing Protein Phosphatase 1 (PP1)-mediated dephosphorylation of the MCM complex PMID: 24532715
  10. Rif1 has important physiological roles in preventing a cell cycle arrest to incipient or small single stranded DNA lesions occurring on chromosomes, particularly on chromosome ends. PMID: 22194703
  11. Saccharomyces cerevisiae Rif1 is an important telomeric factor with an anti-checkpoint role. PMID: 22194704
  12. data supported a model in which Pfa4-dependent palmitoylation of Rif1 anchored it to the inner nuclear membrane, influencing its role in heterochromatin dynamics PMID: 21844336

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Subcellular Location Nucleus. Chromosome, telomere. Note=Localizes to telomeres. Telomere association begins in G1/S, attains a peak during late G2/S phase of the cell cycle, and is lost during telophase. Localization to telomeres may be increased by telomere uncapping caused by expression of a mutant telomerase RNA subunit (TLC1).
Protein Families RIF1 family
Database Links

KEGG: sce:YBR275C

STRING: 4932.YBR275C

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