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| Code | CSB-PA302501XA01HKQ |
| Size | Enquire |
| Have Questions? | Leave a Message or Start an on-line Chat |
| Full Product Name | Rabbit anti-Human immunodeficiency virus type 1 group M subtype B (isolate HXB3) (HIV-1) vpu Polyclonal antibody |
| Uniprot No. | P69700 |
| Target Names | vpu |
| Alternative Names | vpu antibody; Protein Vpu antibody; U ORF protein antibody; Viral protein U antibody |
| Raised in | Rabbit |
| Species Reactivity | Human immunodeficiency virus type 1 group M subtype B (isolate HXB3) (HIV-1) |
| Immunogen | Recombinant Human immunodeficiency virus type 1 group M subtype B (isolate HXB3) (HIV-1) vpu protein |
| Immunogen Species | Human immunodeficiency virus type 1 group M subtype B (isolate HXB3) (HIV-1) |
| Conjugate | Non-conjugated |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purification Method | Protein A/G |
| Concentration | It differs from different batches. Please contact us to confirm it. |
| Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Form | Liquid |
| Tested Applications | ELISA, WB (ensure identification of antigen) |
| Protocols | ELISA Protocol |
| Troubleshooting and FAQs | Antibody FAQs |
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Value-added Deliverables | ① 200ug * antigen (positive control); ② 1ml * Pre-immune serum (negative control); |
| Quality Guarantee | ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection; ② ELISA titer can be guaranteed 1: 64,000; ③ WB validation with antigen can be guaranteed positive; |
| Lead Time | Made-to-order (14-16 weeks) |
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| Function (From Uniprot) |
Enhances virion budding by targeting host CD4 and Tetherin/BST2 to proteasome degradation. Degradation of CD4 prevents any unwanted premature interactions between viral Env and its host receptor CD4 in the endoplasmic reticulum. Degradation of antiretroviral protein Tetherin/BST2 is important for virion budding, as BST2 tethers new viral particles to the host cell membrane. Mechanistically, Vpu bridges either CD4 or BST2 to BTRC, a substrate recognition subunit of the Skp1/Cullin/F-box protein E3 ubiquitin ligase, induces their ubiquitination and subsequent proteasomal degradation. The alteration of the E3 ligase specificity by Vpu seems to promote the degradation of host IKBKB, leading to NF-kappa-B down-regulation and subsequent apoptosis. Ion channel activity has also been suggested, however, formation of cation-selective channel has been reconstituted ex-vivo in lipid bilayers. It is thus unsure that this activity plays a role in vivo. |
| Subcellular Location | Host membrane; Single-pass type I membrane protein. |
| Protein Families | HIV-1 VPU protein family |
