Human β-defensins 3 ELISA Kit

Instructions
Code CSB-E14187h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name defensin, beta 103A
Alternative Names BD 3 ELISA Kit; BD-3 ELISA Kit; BD3 ELISA Kit; beta 103 ELISA Kit; Beta defensin 103A ELISA Kit; Beta defensin 103A precursor ELISA Kit; Beta defensin 3 ELISA Kit; Beta-defensin 103 ELISA Kit; Beta-defensin 3 ELISA Kit; D103A_HUMAN ELISA Kit; DEF B3 ELISA Kit; DEFB 103 ELISA Kit; DEFB 103A ELISA Kit; DEFB 3 ELISA Kit; DEFB-3 ELISA Kit; DEFB103 ELISA Kit; DEFB103A ELISA Kit; DEFB103B ELISA Kit; DEFB3 ELISA Kit; Defensin ELISA Kit; Defensin beta 103A ELISA Kit; Defensin beta 3 ELISA Kit; Defensin like protein ELISA Kit; Defensin-like protein ELISA Kit; HBD 3 ELISA Kit; hBD-3 ELISA Kit; HBD3 ELISA Kit; HBP 3 ELISA Kit; HBP3 ELISA Kit; mBD3 ELISA Kit
Abbreviation DEFB103A
Uniprot No. P81534
Species Homo sapiens (Human)
Sample Types serum, plasma, urine, cell culture supernates, tissue homogenates
Detection Range 125 pg/mL-8000 pg/mL
Sensitivity 31.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human β-defensins 3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %96
Range %91-101
1:2Average %98
Range %92-104
1:4Average %92
Range %87-96
1:8Average %90
Range %86-94
Recovery
The recovery of human β-defensins 3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8782-94
EDTA plasma (n=4)10298-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
80002.339 2.508 2.424 2.270
40001.833 1.869 1.851 1.697
20001.185 1.198 1.192 1.038
10000.699 0.687 0.693 0.539
5000.375 0.392 0.384 0.230
2500.294 0.281 0.288 0.134
1250.208 0.211 0.210 0.056
00.153 0.155 0.154  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Q&A and Customer Reviews

 Q&A
Q:

We want to use pbs buffer 7.4 as sample instead of serum/plasma etc. Would this affect the results? Can you give us any protocol/suggestion? We need an urgent reply:CSB-E14948h Human Antibacterial Peptide LL-37 ELISA Kit and CSB-E14187h Human β-defensins 3 ELISA Kit.

A:
Thanks for your inquiry.Sorry we did not ever tested PBS 7.4 as sample and we are not sure how it will go on.PBS is mainly composed of phosphate.
Normally,there is no detection target in PBS.May I know if you were trying to say that the customer want to use PBS 7.4 as the sample diluent,not the mentioned serum,plasma sample?The kit is equiped with sample diluent in it and we suggest the customer use the sample diluent in the kit directly.
If further questions,pls let us know.

Target Data

Function Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed.
Gene References into Functions
  1. this study shows that hBD3 amplifies the response to bacterial DNA in both mouse and human immune cells in a TLR9-dependent manner PMID: 28102569
  2. the role of DEFB103 gene copy number variation (CNV) in ankylosing spondylitis (AS) susceptibility, was investigated. PMID: 26224324
  3. It may be concluded hepatitis B virus up-regulates HBD-3 and A3G expression in vivo and in vitro in placental trophoblast and lack of this up-regulation is possibly associated with intrauterine transmission of hepatitis B. PMID: 25196417
  4. The encoded peptide displays antimicrobial activity against S. aureus and E. faecium. PMID: 11085990
  5. these observations suggest that there may be an interracial difference in DEFB4/103A copy numbers between admixed populations and a relationship between DEFB1 single nucleotide polymorphisms and DEFB4/103A copy number variation PMID: 23194186
  6. Human beta-defensin 3 peptide is increased and redistributed in Crohn's ileitis PMID: 23511030
  7. study demonstrates that HBD2 and 3 activate plasmacytoid dendritic cells by enhancing the intracellular uptake of CpG and self DNA and promote DNA-induced IFN-alpha production in a TLR9-dependent manner PMID: 23776172
  8. Mapping of key residues in the interaction between human Beta-defensin 3 and CXCR4 reveals key defensin motifs involved in protein binding. PMID: 23659571
  9. These results suggest an important role for hBD3 in inducing dendritic cell activation, migration, and polarization. PMID: 22951718
  10. Using a large, unique cohort of pediatric CA-SAB, this study found no significant association between DEFB1 genetic variation or DEFB4/DEFB103 gene copy number and susceptibility for Staphylococcus aureus bacteremia PMID: 22384213
  11. Human beta-defensin 3 exhibits further functions than antimicrobial peptide activity in cultured bone cells, including stimulation of proliferation and differentiation. PMID: 21520074
  12. Data show that high-glucose conditions inhibited the BD3 expression of epidermal keratinocytes. PMID: 21442129
  13. hBD-1 might function as a tumor suppressor gene in oral squamous cell carcinoma, while hBD-2 and -3 might be protooncogenes. PMID: 21280982
  14. extraplacental membranes can react differentialy to the arrival of E. coli, secreting HBD2 and HBD3 mainly in the choriodecidua region. PMID: 21122132
  15. PPARgamma regulates the 1,25D-induced hBD-3 and cathelicidin expression in keratinocytes through the regulation of AP-1 and p38 activity. PMID: 20970965
  16. hBD3 represents a novel NF-kappaB-regulated mediator of CCR7 expression and anti-apoptotic pathways. PMID: 21071608
  17. Inducibility of HBD3 influences the severity of Gram-positive skin infection in humans in vivo. PMID: 20404083
  18. Data show that beta-defensin cluster (DEFB4, DEFB103 and DEFB104) varied between 2 and 9 copies per genome, and high copy numbers (>4) were underrepresented among patients, suggesting that increased copy numbers could protect from CD. PMID: 20483368
  19. Human pulmonary cells produce hBD-3 upon L. pneumophila infection via a TLR-JNK-AP-1-dependent pathway which may contribute to an efficient innate immune defense. PMID: 20615218
  20. The beta-defensin antimicrobial peptides hBD1, hBD2 and hBD3 were expressed by immature dendritic cells as well as gingival epithelial cells. PMID: 20618959

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Subcellular Location Secreted
Protein Families Beta-defensin family
Tissue Specificity Highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression in salivary gland, bone marrow, colon, stomach, polyp and larynx. No expression in small intestine.
Database Links

HGNC: 15967

OMIM: 606611

KEGG: hsa:414325

STRING: 9606.ENSP00000324633

UniGene: Hs.283082

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