Human β-defensins 3 ELISA Kit

Code CSB-E14187h
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P81534
Lead Time 3-5 working days
Abbreviation DEFB103A
Target Name defensin, beta 103A
Alias DEFB103, DEFB3, HBD-3, HBD3, HBP-3, HBP3, beta-defensin 3|defensin, beta 3
Species Homo sapiens (Human)
Sample Types serum, plasma, urine, cell culture supernates, tissue homogenates
Detection Range 125 pg/mL-8000 pg/mL
Sensitivity 31.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 103B, which has broad spectrum antimicrobial activity and may play an important role in innate epithelial defense.
HGNC 15967
RGD 1561378
MGI 2675345
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human β-defensins 3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %96
Range %91-101
1:2Average %98
Range %92-104
1:4Average %92
Range %87-96
1:8Average %90
Range %86-94
Recovery
The recovery of human β-defensins 3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8782-94
EDTA plasma (n=4)10298-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
80002.339 2.508 2.424 2.270
40001.833 1.869 1.851 1.697
20001.185 1.198 1.192 1.038
10000.699 0.687 0.693 0.539
5000.375 0.392 0.384 0.230
2500.294 0.281 0.288 0.134
1250.208 0.211 0.210 0.056
00.153 0.155 0.154  
References
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Function Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed.
Subcellular Location Secreted
Protein Families Beta-defensin family
Tissue Specificity Highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression in salivary gland, bone marrow, colon, stomach, polyp and larynx. No expression in small intestine.
Database Links

HGNC: 15967

OMIM: 606611

KEGG: hsa:414325

STRING: 9606.ENSP00000324633

UniGene: Hs.283082

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