Human Aldo-keto reductase family 1 member B10(AKR1B10) ELISA kit

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Name: Human Aldo-keto reductase family 1 member B10(AKR1B10) ELISA kit
Brand: CUSABIO
SKU: CSB-EL001540HU

Product Details

Description

CUSABIO’s Human Aldo-keto reductase family 1 member B10 (AKR1B10) ELISA kit is a sandwich immunoassay specifically designed and validated for the quantitative detection of AKR1B10 in the serum, plasma, or tissue homogenates. It is not intended for diagnostic use. This assay kit was designed and optimized for metabolic research use in humans. The kit has undergone rigorous quality control in multiple parameters, including sensitivity, specificity, precision, linearity, recovery, and inter-batch difference. Refer to the product instructions for more details.

This assay employs the quantitative sandwich enzyme immunoassay technique, in which AKR1B10 in the samples or standards are sandwiched between pre-coated AKR1B10 antibody and Biotin-conjugated AKR1B10 antibody. HRP-avidin is then added into the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of AKR1B10 bound in the initial step. The color development is stopped upon adding the stop solution, and the intensity of the color is measured at 450 nm via a microplate reader. The levels of AKR1B10 in the samples can be determined by referring to the O.D. (optical density) of the samples to the standard curve.

AKR1B10, also called aldose reductase-like-1 (ARL-1), is a secretory protein mainly expressed in the normal human small intestine and colon. Overexpression of AKR1B10 has been found in several types of human cancers. AKR1B10 displays more restricted lipid substrate specificity than that of general human Aldo-ketoreductases. Metabolism of these lipid substrates including farnesol, geranylgeranial, retinal, and carbonyls, plays a critical role in promoting carcinogenesis.

Target Name

aldo-keto reductase family 1, member B10 (aldose reductase)

Alternative Names

AK1BA_HUMAN ELISA Kit; AKR1B10 ELISA Kit; AKR1B11 ELISA Kit; AKR1B12 ELISA Kit; Aldo keto reductase family 1 member B10 ELISA Kit; aldo keto reductase family 1 member B11 ELISA Kit; Aldo-keto reductase family 1 member B10 (aldose reductase) ELISA Kit; Aldo-keto reductase family 1 member B10 ELISA Kit; Aldo-keto reductase family 1 member B11 (aldose reductase-like) ELISA Kit; aldose reductase like 1 ELISA Kit; Aldose reductase like ELISA Kit; aldose reductase like peptide ELISA Kit; Aldose reductase related protein ELISA Kit; Aldose reductase-like ELISA Kit; Aldose reductase-related protein ELISA Kit; ALDRLn ELISA Kit; ARL 1 ELISA Kit; ARL-1 ELISA Kit; ARL1 ELISA Kit; ARL1 Human small intestine reductase ELISA Kit; ARP ELISA Kit; hARP ELISA Kit; HIS ELISA Kit; HSI ELISA Kit; SI reductase ELISA Kit; Small intestine reductase ELISA Kit

Abbreviation

AKR1B10

Uniprot No.

O60218

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.625 ng/mL-40 ng/mL

Sensitivity

0.156 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human AKR1B10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-99
1:2	Average %	97
	Range %	91-100
1:4	Average %	89
	Range %	85-96
1:8	Average %	87
	Range %	83-92

Recovery

The recovery of human AKR1B10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-97
EDTA plasma (n=4)	85	81-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.143	2.253	2.198	2.027
20	1.544	1.534	1.539	1.368
10	1.077	1.164	1.121	0.950
5	0.774	0.792	0.783	0.612
2.5	0.513	0.523	0.518	0.347
1.25	0.368	0.398	0.383	0.212
0.625	0.273	0.281	0.277	0.106
0	0.170	0.172	0.171

Materials provided

A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human AKR1B10 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled AKR1B10 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

Materials not provided

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

7-14 working days

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Target Background

Function (From Uniprot)	Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs.
Gene References into Functions	Immunohistochemistry showed that the expression of AKR1B10 was increased in tumor tissue from patients with early-stage HCC. PMID: 30328412 These results suggest that AKR1B10 is involved in HBV-related hepatocarcinogenesis, but its high expression could predict low risk of early tumor recurrence in patients with HBV-related hepatocellular carcinoma (HCC)after liver resection. PMID: 28181486 AKR1B10 is upregulated in mucinous cystic pancreatic tumors and may be used to improve non-operative diagnosis. PMID: 29079172 A combined gene expression signature of AKR1B10 (low) and AKR1B1 (high) showed a better prognostic stratification of CRC patients independent of confounding factors. PMID: 28929377 Our study demonstrated not only the dysfunction of the AKR1B10 gene in lipid metabolizing but also its important role in the overproliferation and migration of keratinocyte, which provided evidence for further therapeutic uses for psoriasis. PMID: 29204449 Data show that cells with higher levels of aldo-keto reductases AKR1B1 and/or AKR1B10 (AKR1Bs) were more sensitive to 2-deoxyglucose (2DG). PMID: 29617059 Results demonstrate that Akr7a3 mRNA and protein levels are consistently co-expressed along with Akr1b10, in both experimental rat liver carcinogenesis and some human hepatocellular carcinoma samples. PMID: 29383608 AKR1B10 overexpression is an independent poor prognostic biomarker for oral squamous cell carcinoma. PMID: 28301069 These data suggest that AKR1B10 promotes breast cancer metastasis PMID: 27248472 Several antioxidant response elements-like sequences in the 5'-flanking region up to -3282 bp of the AKR1B10 gene plays an important role in AKR1B10 gene regulation by various Nrf2-mediating stimuli. PMID: 28219640 This study presents novel evidence that AKR1B10 protects colon cells from DNA damage induced by electrophilic carbonyl compounds. PMID: 26969882 the highly efficient, retinaldehyde-specific aldo-keto reductase enzyme AKR1B10 is up-regulated in keloid epidermis(KE) and its induced overexpression in normal skin keratinocytes affects the classical RA pathway, recreating KE expression patterns PMID: 27025872 Data suggest that expression of STAR (steroidogenic acute regulatory protein) and AKR1B10 (aldo-keto reductase family 1, member B10) is down-regulated in high-grade versus low-grade endometrial tumors; expression of AKR1B10 correlates with body mass index, with up-regulation of expression of AKR1B10 in obese patients with endometrial tumors. PMID: 28232277 Result indicate that the differential scanning fluorimetry (DSF) method is useful for enzyme inhibitor drug screening for the AKR superfamily, including AKR1B10 and a structurally similar isoform AKR1B1. PMID: 28003428 Chronic hepatitis C patients expressing high levels of hepatic AKR1B10 had an increased risk of HCC development even after sustained virological response PMID: 27672277 Various degrees of AKR1B10 upregulation in the liver were observed in patients with chronic HCV infection, and high AKR1B10 expression could be a novel pre- dictor of HCC. PMID: 26758591 AKR1B10 is up-regulated with cisplatin resistance in gastrointestinal cancer cells. PMID: 27417252 AKR1B10 mRNA and protein levels were higher in primary hepatocellular carcinoma (PHC) tissues than in peri-tumor tissues. PMID: 26948042 AKR1B10 is overexpressed in nasopharyngeal hyperplasia, benign tumors, and carcinomas, being a potential new biomarker. PMID: 26835713 Even at early stages of malignant transformationa considerable increase in AKR1B10 mRNA content was observed in 80% of tumors PMID: 27239845 It would appear that hepatitis C virus infection alone increases AKR1B10 expression, which manifests itself as enhanced urinary excretion of polyols with reduced urinary excretion of their corresponding hexoses. PMID: 25487531 Our data suggest that genetic variants in the AKR1B10 locus may influence human eating behavior. PMID: 25887478 The three-dimensional structure of AKR1B10 with sulindac. PMID: 25532697 The co-introduction of the c-Jun protein resulted in a decrease in the mRNA levels and promoter activity of AKR1B10. PMID: 25463304 Autophagy and AKR1B10 contribute to the defense system. PMID: 25289770 AKR1B10 is a doxorubicin-resistance gene in the gastric cancer cells, and is responsible for elevating the migrating and invasive potentials of the cells through induction of MMP2. PMID: 25686905 Upregulation of AKR1B10 is associated with cisplatin resistance in lung cancer. PMID: 25156503 AKR1B10 is a unique enzyme involved in pancreatic carcinogenesis via modulation of the Kras-E-cadherin pathway. PMID: 25304374 AKR1B10 may have an important role in the development and progression of diabetic nephropathy. PMID: 23975544 A significant correlation between the expression of AKR1B10 mRNA and protein levels as well as AKR1B10 copy number was observered, which suggest that AKR1B10 may play a role in AFB1-related hepatocarcinogenesis. PMID: 24391771 Results suggest that decreased expression of AKR1B10 could disrupt the tumor suppressive function of p53, which result in decreased survival in colorectal cancer patients. PMID: 24140838 AKR1B10, a member of Aldo-keto reductase family, was shown to be abundantly located in the filtering cells among a catalog of proteins.in ducal carcinoma of the breast. PMID: 23912490 AKR1B10 is a unique biomarker involved in hepatocellular carcinogenesis PMID: 24656094 AKR1B10 protein gene expression is a valuable indicator in patients diagnosed with gastric cancer. PMID: 24406159 Lys-233, Glu-236, and Lys-240 in helix 10 mediate interaction of AKR1B10 with HSP90alpha and regulate AKR1B10 secretion. PMID: 24217247 A hydrogen bond stabilized active site tryptophan conformation restricts inhibitor access in AKR1B1 compared with the more open AKR1B10 active site. PMID: 24100137 our data suggest that post-chemotherapy AKR1B10 expression may be associated with a poor prognosis in patients who received carboplatin-gemcitabine combination chemotherapy and underwent cystectomy PMID: 22198799 The gene expression of AKR1B10 at the mRNA level was significantly increased. PMID: 23146748 AKR1B10 was the most efficient catalyst of the stoichiometric two-electron reduction of BQ. PMID: 22498646 AKR1B10 was up-regulated in association with serum alpha-fetoprotein, and was an independent risk factor for hepatocellular carcinoma in chronic hepatitis C patients. PMID: 22681639 AKR1B10 is a unique enzyme involved in pancreatic carcinogenesis possibly via modulation of cell apoptosis and protein prenylation. PMID: 22222635 results suggest that AKR1B10 is up-regulated by EGF and insulin through AP-1 mitogenic signalling and may be implicated in hepatocarcinogenesis PMID: 22329800 AKR1B10 overexpression is a prominent feature in both hereditary and sporadic papillary renal cell carcinoma and is upregulated in cell lines carrying a fumarate hydratase mutation. PMID: 22014576 AKR1B10 might promote proliferation, inhibit apoptosis and induce malignant transformation of hepatocytes by regulating the expression level of some tumor-related genes. PMID: 20943077 Confirm prognostic value of AKR1B10 in hepatocellular carcinoma and conclude that high expression reflects less aggressive tumour behaviour. PMID: 21645211 High AKR1B10 secretion is associated with neoplasms. PMID: 21585341 AKR1B10 is associated with smoking and smoking-related non-small-cell lung cancer. PMID: 21672310 the enzyme activity of AKR1B10 and AKR1B1 toward alpha, beta-unsaturated carbonyl compounds with cellular and dietary origins PMID: 21329684 Nrf2 is one of the major factors involved in the AKR1B10 gene regulation PMID: 21277289 overexpression and silencing of AKR1B10 decreased and increased, respectively, susceptibility to cytotoxic effects of MMC and 4-hydroxy-2-nonenal, which was formed as a product of lipid peroxidation by MMC treatment. PMID: 21317765 Show More Hide All
Subcellular Location	Lysosome, Secreted
Protein Families	Aldo/keto reductase family
Tissue Specificity	Found in many tissues. Highly expressed in small intestine, colon and adrenal gland.
Database Links	HGNC: 382 OMIM: 604707 KEGG: hsa:57016 STRING: 9606.ENSP00000352584 UniGene: Hs.116724

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Code	CSB-EL001540HU
Size	96T,5×96T,10×96T
Trial Size
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