Human Aldo-keto reductase family 1 member B10(AKR1B10) ELISA kit

Code CSB-EL001540HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
aldo-keto reductase family 1, member B10 (aldose reductase)
Alternative Names
AK1BA_HUMAN ELISA Kit; AKR1B10 ELISA Kit; AKR1B11 ELISA Kit; AKR1B12 ELISA Kit; Aldo keto reductase family 1 member B10 ELISA Kit; aldo keto reductase family 1 member B11 ELISA Kit; Aldo-keto reductase family 1 member B10 (aldose reductase) ELISA Kit; Aldo-keto reductase family 1 member B10 ELISA Kit; Aldo-keto reductase family 1 member B11 (aldose reductase-like) ELISA Kit; aldose reductase like 1 ELISA Kit; Aldose reductase like ELISA Kit; aldose reductase like peptide ELISA Kit; Aldose reductase related protein ELISA Kit; Aldose reductase-like ELISA Kit; Aldose reductase-related protein ELISA Kit; ALDRLn ELISA Kit; ARL 1 ELISA Kit; ARL-1 ELISA Kit; ARL1 ELISA Kit; ARL1 Human small intestine reductase ELISA Kit; ARP ELISA Kit; hARP ELISA Kit; HIS ELISA Kit; HSI ELISA Kit; SI reductase ELISA Kit; Small intestine reductase ELISA Kit
Uniprot No.
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.625 ng/mL-40 ng/mL
0.156 ng/mL
Assay Time
Sample Volume
Detection Wavelength
450 nm
Research Area
Assay Principle
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human AKR1B10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %90
Range %85-99
1:2Average %97
Range %91-100
1:4Average %89
Range %85-96
1:8Average %87
Range %83-92
The recovery of human AKR1B10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9389-97
EDTA plasma (n=4)8581-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
402.143 2.253 2.198 2.027
201.544 1.534 1.539 1.368
101.077 1.164 1.121 0.950
50.774 0.792 0.783 0.612
2.50.513 0.523 0.518 0.347
1.250.368 0.398 0.383 0.212
0.6250.273 0.281 0.277 0.106
00.170 0.172 0.171  
Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human AKR1B10 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled AKR1B10 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
and FAQs
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

This Human AKR1B10 ELISA Kit was designed for the quantitative measurement of Human AKR1B10 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .

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Target Background

(From Uniprot)
Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays strong enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. Plays a critical role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls). Displays no reductase activity towards glucose.
Gene References into Functions
  1. Immunohistochemistry showed that the expression of AKR1B10 was increased in tumor tissue from patients with early-stage HCC. PMID: 30328412
  2. These results suggest that AKR1B10 is involved in HBV-related hepatocarcinogenesis, but its high expression could predict low risk of early tumor recurrence in patients with HBV-related hepatocellular carcinoma (HCC)after liver resection. PMID: 28181486
  3. AKR1B10 is upregulated in mucinous cystic pancreatic tumors and may be used to improve non-operative diagnosis. PMID: 29079172
  4. A combined gene expression signature of AKR1B10 (low) and AKR1B1 (high) showed a better prognostic stratification of CRC patients independent of confounding factors. PMID: 28929377
  5. Our study demonstrated not only the dysfunction of the AKR1B10 gene in lipid metabolizing but also its important role in the overproliferation and migration of keratinocyte, which provided evidence for further therapeutic uses for psoriasis. PMID: 29204449
  6. Data show that cells with higher levels of aldo-keto reductases AKR1B1 and/or AKR1B10 (AKR1Bs) were more sensitive to 2-deoxyglucose (2DG). PMID: 29617059
  7. Results demonstrate that Akr7a3 mRNA and protein levels are consistently co-expressed along with Akr1b10, in both experimental rat liver carcinogenesis and some human hepatocellular carcinoma samples. PMID: 29383608
  8. AKR1B10 overexpression is an independent poor prognostic biomarker for oral squamous cell carcinoma. PMID: 28301069
  9. These data suggest that AKR1B10 promotes breast cancer metastasis PMID: 27248472
  10. Several antioxidant response elements-like sequences in the 5'-flanking region up to -3282 bp of the AKR1B10 gene plays an important role in AKR1B10 gene regulation by various Nrf2-mediating stimuli. PMID: 28219640
  11. This study presents novel evidence that AKR1B10 protects colon cells from DNA damage induced by electrophilic carbonyl compounds. PMID: 26969882
  12. the highly efficient, retinaldehyde-specific aldo-keto reductase enzyme AKR1B10 is up-regulated in keloid epidermis(KE) and its induced overexpression in normal skin keratinocytes affects the classical RA pathway, recreating KE expression patterns PMID: 27025872
  13. Data suggest that expression of STAR (steroidogenic acute regulatory protein) and AKR1B10 (aldo-keto reductase family 1, member B10) is down-regulated in high-grade versus low-grade endometrial tumors; expression of AKR1B10 correlates with body mass index, with up-regulation of expression of AKR1B10 in obese patients with endometrial tumors. PMID: 28232277
  14. Result indicate that the differential scanning fluorimetry (DSF) method is useful for enzyme inhibitor drug screening for the AKR superfamily, including AKR1B10 and a structurally similar isoform AKR1B1. PMID: 28003428
  15. Chronic hepatitis C patients expressing high levels of hepatic AKR1B10 had an increased risk of HCC development even after sustained virological response PMID: 27672277
  16. Various degrees of AKR1B10 upregulation in the liver were observed in patients with chronic HCV infection, and high AKR1B10 expression could be a novel pre- dictor of HCC. PMID: 26758591
  17. AKR1B10 is up-regulated with cisplatin resistance in gastrointestinal cancer cells. PMID: 27417252
  18. AKR1B10 mRNA and protein levels were higher in primary hepatocellular carcinoma (PHC) tissues than in peri-tumor tissues. PMID: 26948042
  19. AKR1B10 is overexpressed in nasopharyngeal hyperplasia, benign tumors, and carcinomas, being a potential new biomarker. PMID: 26835713
  20. Even at early stages of malignant transformationa considerable increase in AKR1B10 mRNA content was observed in 80% of tumors PMID: 27239845
  21. It would appear that hepatitis C virus infection alone increases AKR1B10 expression, which manifests itself as enhanced urinary excretion of polyols with reduced urinary excretion of their corresponding hexoses. PMID: 25487531
  22. Our data suggest that genetic variants in the AKR1B10 locus may influence human eating behavior. PMID: 25887478
  23. The three-dimensional structure of AKR1B10 with sulindac. PMID: 25532697
  24. The co-introduction of the c-Jun protein resulted in a decrease in the mRNA levels and promoter activity of AKR1B10. PMID: 25463304
  25. Autophagy and AKR1B10 contribute to the defense system. PMID: 25289770
  26. AKR1B10 is a doxorubicin-resistance gene in the gastric cancer cells, and is responsible for elevating the migrating and invasive potentials of the cells through induction of MMP2. PMID: 25686905
  27. Upregulation of AKR1B10 is associated with cisplatin resistance in lung cancer. PMID: 25156503
  28. AKR1B10 is a unique enzyme involved in pancreatic carcinogenesis via modulation of the Kras-E-cadherin pathway. PMID: 25304374
  29. AKR1B10 may have an important role in the development and progression of diabetic nephropathy. PMID: 23975544
  30. A significant correlation between the expression of AKR1B10 mRNA and protein levels as well as AKR1B10 copy number was observered, which suggest that AKR1B10 may play a role in AFB1-related hepatocarcinogenesis. PMID: 24391771
  31. Results suggest that decreased expression of AKR1B10 could disrupt the tumor suppressive function of p53, which result in decreased survival in colorectal cancer patients. PMID: 24140838
  32. AKR1B10, a member of Aldo-keto reductase family, was shown to be abundantly located in the filtering cells among a catalog of ducal carcinoma of the breast. PMID: 23912490
  33. AKR1B10 is a unique biomarker involved in hepatocellular carcinogenesis PMID: 24656094
  34. AKR1B10 protein gene expression is a valuable indicator in patients diagnosed with gastric cancer. PMID: 24406159
  35. Lys-233, Glu-236, and Lys-240 in helix 10 mediate interaction of AKR1B10 with HSP90alpha and regulate AKR1B10 secretion. PMID: 24217247
  36. A hydrogen bond stabilized active site tryptophan conformation restricts inhibitor access in AKR1B1 compared with the more open AKR1B10 active site. PMID: 24100137
  37. our data suggest that post-chemotherapy AKR1B10 expression may be associated with a poor prognosis in patients who received carboplatin-gemcitabine combination chemotherapy and underwent cystectomy PMID: 22198799
  38. The gene expression of AKR1B10 at the mRNA level was significantly increased. PMID: 23146748
  39. AKR1B10 was the most efficient catalyst of the stoichiometric two-electron reduction of BQ. PMID: 22498646
  40. AKR1B10 was up-regulated in association with serum alpha-fetoprotein, and was an independent risk factor for hepatocellular carcinoma in chronic hepatitis C patients. PMID: 22681639
  41. AKR1B10 is a unique enzyme involved in pancreatic carcinogenesis possibly via modulation of cell apoptosis and protein prenylation. PMID: 22222635
  42. results suggest that AKR1B10 is up-regulated by EGF and insulin through AP-1 mitogenic signalling and may be implicated in hepatocarcinogenesis PMID: 22329800
  43. AKR1B10 overexpression is a prominent feature in both hereditary and sporadic papillary renal cell carcinoma and is upregulated in cell lines carrying a fumarate hydratase mutation. PMID: 22014576
  44. AKR1B10 might promote proliferation, inhibit apoptosis and induce malignant transformation of hepatocytes by regulating the expression level of some tumor-related genes. PMID: 20943077
  45. Confirm prognostic value of AKR1B10 in hepatocellular carcinoma and conclude that high expression reflects less aggressive tumour behaviour. PMID: 21645211
  46. High AKR1B10 secretion is associated with neoplasms. PMID: 21585341
  47. AKR1B10 is associated with smoking and smoking-related non-small-cell lung cancer. PMID: 21672310
  48. the enzyme activity of AKR1B10 and AKR1B1 toward alpha, beta-unsaturated carbonyl compounds with cellular and dietary origins PMID: 21329684
  49. Nrf2 is one of the major factors involved in the AKR1B10 gene regulation PMID: 21277289
  50. overexpression and silencing of AKR1B10 decreased and increased, respectively, susceptibility to cytotoxic effects of MMC and 4-hydroxy-2-nonenal, which was formed as a product of lipid peroxidation by MMC treatment. PMID: 21317765

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Subcellular Location
Lysosome. Secreted. Note=Secreted through a lysosome-mediated non-classical pathway.
Protein Families
Aldo/keto reductase family
Tissue Specificity
Found in many tissues. Highly expressed in small intestine, colon and adrenal gland.
Database Links

HGNC: 382

OMIM: 604707

KEGG: hsa:57016

STRING: 9606.ENSP00000352584

UniGene: Hs.116724

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