CUSABIO’s Human Aldo-keto reductase family 1 member B10 (AKR1B10) ELISA kit is a sandwich immunoassay specifically designed and validated for the quantitative detection of AKR1B10 in the serum, plasma, or tissue homogenates. It is not intended for diagnostic use. This assay kit was designed and optimized for metabolic research use in humans. The kit has undergone rigorous quality control in multiple parameters, including sensitivity, specificity, precision, linearity, recovery, and inter-batch difference. Refer to the product instructions for more details.
This assay employs the quantitative sandwich enzyme immunoassay technique, in which AKR1B10 in the samples or standards are sandwiched between pre-coated AKR1B10 antibody and Biotin-conjugated AKR1B10 antibody. HRP-avidin is then added into the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of AKR1B10 bound in the initial step. The color development is stopped upon adding the stop solution, and the intensity of the color is measured at 450 nm via a microplate reader. The levels of AKR1B10 in the samples can be determined by referring to the O.D. (optical density) of the samples to the standard curve.
AKR1B10, also called aldose reductase-like-1 (ARL-1), is a secretory protein mainly expressed in the normal human small intestine and colon. Overexpression of AKR1B10 has been found in several types of human cancers. AKR1B10 displays more restricted lipid substrate specificity than that of general human Aldo-ketoreductases. Metabolism of these lipid substrates including farnesol, geranylgeranial, retinal, and carbonyls, plays a critical role in promoting carcinogenesis.