Recombinant Human Aldo-keto reductase family 1 member B10(AKR1B10)

Code CSB-EP001540HU
Size US$1726
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names AKR1B10
Uniprot No. O60218
Research Area Signal Transduction
Alternative Names AK1BA_HUMAN; AKR1B10; AKR1B11; AKR1B12; Aldo keto reductase family 1 member B10; aldo keto reductase family 1 member B11; Aldo-keto reductase family 1 member B10 (aldose reductase); Aldo-keto reductase family 1 member B10; Aldo-keto reductase family 1 member B11 (aldose reductase-like); aldose reductase like 1; Aldose reductase like; aldose reductase like peptide; Aldose reductase related protein; Aldose reductase-like; Aldose reductase-related protein; ALDRLn; ARL 1; ARL-1; ARL1; ARL1 Human small intestine reductase; ARP; hARP; HIS; HSI; SI reductase; Small intestine reductase
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-316aa
Target Protein Sequence MATFVELSTKAKMPIVGLGTWKSPLGKVKEAVKVAIDAGYRHIDCAYVYQNEHEVGEAIQEKIQEKAVKREDLFIVSKLWPTFFERPLVRKAFEKTLKDLKLSYLDVYLIHWPQGFKSGDDLFPKDDKGNAIGGKATFLDAWEAMEELVDEGLVKALGVSNFSHFQIEKLLNKPGLKYKPVTNQVECHPYLTQEKLIQYCHSKGITVTAYSPLGSPDRPWAKPEDPSLLEDPKIKEIAAKHKKTAAQVLIRFHIQRNVIVIPKSVTPARIVENIQVFDFKLSDEEMATILSFNRNWRACNVLQSSHLEDYPFNAEY
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 63.0kDa
Protein Length Full Length
Tag Info N-terminal GST-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs.
Gene References into Functions
  1. Immunohistochemistry showed that the expression of AKR1B10 was increased in tumor tissue from patients with early-stage HCC. PMID: 30328412
  2. These results suggest that AKR1B10 is involved in HBV-related hepatocarcinogenesis, but its high expression could predict low risk of early tumor recurrence in patients with HBV-related hepatocellular carcinoma (HCC)after liver resection. PMID: 28181486
  3. AKR1B10 is upregulated in mucinous cystic pancreatic tumors and may be used to improve non-operative diagnosis. PMID: 29079172
  4. A combined gene expression signature of AKR1B10 (low) and AKR1B1 (high) showed a better prognostic stratification of CRC patients independent of confounding factors. PMID: 28929377
  5. Our study demonstrated not only the dysfunction of the AKR1B10 gene in lipid metabolizing but also its important role in the overproliferation and migration of keratinocyte, which provided evidence for further therapeutic uses for psoriasis. PMID: 29204449
  6. Data show that cells with higher levels of aldo-keto reductases AKR1B1 and/or AKR1B10 (AKR1Bs) were more sensitive to 2-deoxyglucose (2DG). PMID: 29617059
  7. Results demonstrate that Akr7a3 mRNA and protein levels are consistently co-expressed along with Akr1b10, in both experimental rat liver carcinogenesis and some human hepatocellular carcinoma samples. PMID: 29383608
  8. AKR1B10 overexpression is an independent poor prognostic biomarker for oral squamous cell carcinoma. PMID: 28301069
  9. These data suggest that AKR1B10 promotes breast cancer metastasis PMID: 27248472
  10. Several antioxidant response elements-like sequences in the 5'-flanking region up to -3282 bp of the AKR1B10 gene plays an important role in AKR1B10 gene regulation by various Nrf2-mediating stimuli. PMID: 28219640
  11. This study presents novel evidence that AKR1B10 protects colon cells from DNA damage induced by electrophilic carbonyl compounds. PMID: 26969882
  12. the highly efficient, retinaldehyde-specific aldo-keto reductase enzyme AKR1B10 is up-regulated in keloid epidermis(KE) and its induced overexpression in normal skin keratinocytes affects the classical RA pathway, recreating KE expression patterns PMID: 27025872
  13. Data suggest that expression of STAR (steroidogenic acute regulatory protein) and AKR1B10 (aldo-keto reductase family 1, member B10) is down-regulated in high-grade versus low-grade endometrial tumors; expression of AKR1B10 correlates with body mass index, with up-regulation of expression of AKR1B10 in obese patients with endometrial tumors. PMID: 28232277
  14. Result indicate that the differential scanning fluorimetry (DSF) method is useful for enzyme inhibitor drug screening for the AKR superfamily, including AKR1B10 and a structurally similar isoform AKR1B1. PMID: 28003428
  15. Chronic hepatitis C patients expressing high levels of hepatic AKR1B10 had an increased risk of HCC development even after sustained virological response PMID: 27672277
  16. Various degrees of AKR1B10 upregulation in the liver were observed in patients with chronic HCV infection, and high AKR1B10 expression could be a novel pre- dictor of HCC. PMID: 26758591
  17. AKR1B10 is up-regulated with cisplatin resistance in gastrointestinal cancer cells. PMID: 27417252
  18. AKR1B10 mRNA and protein levels were higher in primary hepatocellular carcinoma (PHC) tissues than in peri-tumor tissues. PMID: 26948042
  19. AKR1B10 is overexpressed in nasopharyngeal hyperplasia, benign tumors, and carcinomas, being a potential new biomarker. PMID: 26835713
  20. Even at early stages of malignant transformationa considerable increase in AKR1B10 mRNA content was observed in 80% of tumors PMID: 27239845
  21. It would appear that hepatitis C virus infection alone increases AKR1B10 expression, which manifests itself as enhanced urinary excretion of polyols with reduced urinary excretion of their corresponding hexoses. PMID: 25487531
  22. Our data suggest that genetic variants in the AKR1B10 locus may influence human eating behavior. PMID: 25887478
  23. The three-dimensional structure of AKR1B10 with sulindac. PMID: 25532697
  24. The co-introduction of the c-Jun protein resulted in a decrease in the mRNA levels and promoter activity of AKR1B10. PMID: 25463304
  25. Autophagy and AKR1B10 contribute to the defense system. PMID: 25289770
  26. AKR1B10 is a doxorubicin-resistance gene in the gastric cancer cells, and is responsible for elevating the migrating and invasive potentials of the cells through induction of MMP2. PMID: 25686905
  27. Upregulation of AKR1B10 is associated with cisplatin resistance in lung cancer. PMID: 25156503
  28. AKR1B10 is a unique enzyme involved in pancreatic carcinogenesis via modulation of the Kras-E-cadherin pathway. PMID: 25304374
  29. AKR1B10 may have an important role in the development and progression of diabetic nephropathy. PMID: 23975544
  30. A significant correlation between the expression of AKR1B10 mRNA and protein levels as well as AKR1B10 copy number was observered, which suggest that AKR1B10 may play a role in AFB1-related hepatocarcinogenesis. PMID: 24391771
  31. Results suggest that decreased expression of AKR1B10 could disrupt the tumor suppressive function of p53, which result in decreased survival in colorectal cancer patients. PMID: 24140838
  32. AKR1B10, a member of Aldo-keto reductase family, was shown to be abundantly located in the filtering cells among a catalog of proteins.in ducal carcinoma of the breast. PMID: 23912490
  33. AKR1B10 is a unique biomarker involved in hepatocellular carcinogenesis PMID: 24656094
  34. AKR1B10 protein gene expression is a valuable indicator in patients diagnosed with gastric cancer. PMID: 24406159
  35. Lys-233, Glu-236, and Lys-240 in helix 10 mediate interaction of AKR1B10 with HSP90alpha and regulate AKR1B10 secretion. PMID: 24217247
  36. A hydrogen bond stabilized active site tryptophan conformation restricts inhibitor access in AKR1B1 compared with the more open AKR1B10 active site. PMID: 24100137
  37. our data suggest that post-chemotherapy AKR1B10 expression may be associated with a poor prognosis in patients who received carboplatin-gemcitabine combination chemotherapy and underwent cystectomy PMID: 22198799
  38. The gene expression of AKR1B10 at the mRNA level was significantly increased. PMID: 23146748
  39. AKR1B10 was the most efficient catalyst of the stoichiometric two-electron reduction of BQ. PMID: 22498646
  40. AKR1B10 was up-regulated in association with serum alpha-fetoprotein, and was an independent risk factor for hepatocellular carcinoma in chronic hepatitis C patients. PMID: 22681639
  41. AKR1B10 is a unique enzyme involved in pancreatic carcinogenesis possibly via modulation of cell apoptosis and protein prenylation. PMID: 22222635
  42. results suggest that AKR1B10 is up-regulated by EGF and insulin through AP-1 mitogenic signalling and may be implicated in hepatocarcinogenesis PMID: 22329800
  43. AKR1B10 overexpression is a prominent feature in both hereditary and sporadic papillary renal cell carcinoma and is upregulated in cell lines carrying a fumarate hydratase mutation. PMID: 22014576
  44. AKR1B10 might promote proliferation, inhibit apoptosis and induce malignant transformation of hepatocytes by regulating the expression level of some tumor-related genes. PMID: 20943077
  45. Confirm prognostic value of AKR1B10 in hepatocellular carcinoma and conclude that high expression reflects less aggressive tumour behaviour. PMID: 21645211
  46. High AKR1B10 secretion is associated with neoplasms. PMID: 21585341
  47. AKR1B10 is associated with smoking and smoking-related non-small-cell lung cancer. PMID: 21672310
  48. the enzyme activity of AKR1B10 and AKR1B1 toward alpha, beta-unsaturated carbonyl compounds with cellular and dietary origins PMID: 21329684
  49. Nrf2 is one of the major factors involved in the AKR1B10 gene regulation PMID: 21277289
  50. overexpression and silencing of AKR1B10 decreased and increased, respectively, susceptibility to cytotoxic effects of MMC and 4-hydroxy-2-nonenal, which was formed as a product of lipid peroxidation by MMC treatment. PMID: 21317765
  51. Smoking per se mediates upregulation of AKR1B10 expression in the airway epithelia of healthy smokers with no evidence of lung cancer. PMID: 20705797
  52. The significant decrease of AKR1B10 mRNA in most samples of colorectal cancer could be considered as potential diagnostic marker of this type of cancer. PMID: 20586184
  53. Overexpression of AKR1B10 in early stages of well and moderately differentiated tumors and its downregulation in advanced tumors with low grade of differentiation showed that AKR1B10 may be a marker for differentiation of primary liver tumors. PMID: 20036025
  54. Transgenic aldose reductase plays a novel role regulating the signaling pathways leading to neutrophil-endothelial cell adhesion during acute lung inflammation. PMID: 20007578
  55. role as efficient retinal reductase and consequence in retinoid metabolism PMID: 12732097
  56. Z-2 allele of the aldose reductase gene is a risk factor for the development of diabetic retinopathy in a group of Caucasian participants with Type 2 diabetes. PMID: 15745835
  57. Aldose reductase might play a role in cell cycle progression of A549 tumor cells. PMID: 15928807
  58. Data show tha activation of protein kinase C and tyrosine kinase by 12-O-tetradecanoylphorbol-13-acetate elicits increased promoter activity of aldose reductase gene via nuclear factor kappaB. PMID: 15936242
  59. Data show that human aldose reductase increases atherosclerosis in diabetic mice. PMID: 16127462
  60. crystallographic analysis of human aldose reductase PMID: 16204895
  61. TIMP3, DAPK1 and AKR1B10 are important for squamous cell lung cancer tumorogenesis while AKR1B10 is potential oncogene whereas TIMP3 and DAPK1 are potential tumor suppressor genes. PMID: 17209433
  62. Inhibition of aldose reductase prevented TNF-alpha-induced activation of protein kinase C and NF-kappaB which resulted in the abrogation of Cox-2 mRNA and protein expression PMID: 17300864
  63. associated with tumor recurrence after surgery and keratinization of squamous cell carcinoma in cervical cancer but not endometrial cancer PMID: 17425679
  64. AKR1B10 may regulate cell proliferation and cellular response to additional carbonyl stress PMID: 17597105
  65. a tyrosine residue located in the catalytic site (Tyr48) is a likely candidate to act as proton acceptor upon inhibitor binding, as it occurs deprotonated to a remarkable extent if only the cofactor NADP+ is bound PMID: 17905306
  66. AKR1B10 regulates the stability of acetyl-CoA carboxylase-alpha and is a novel regulator of the biosynthesis of fatty acid, an essential component of the cell membrane, in breast cancer cells PMID: 18056116
  67. structural analysis of the high all-trans-retinaldehyde reductase activity of the tumor marker AKR1B10 PMID: 18087047
  68. The reaction of C299S mutant AKR1B10 is inhibited by fenofibric acid, but manifests pure non-competitive inhibition kinetics that are different from those demonstrated for the wild-type enzyme. PMID: 18325492
  69. AKR1B10 might be useful as a new marker for identification of high lung cancer risk patients in usual interstitial pneumonia. PMID: 18358633
  70. Results describe cathepsin D and aldo-keto reductase 1C2 and 1B10 dysregulation in Barrett's esophagus and esophageal adenocarcinoma. PMID: 18396902
  71. AKR1B10 may play a greater role in lung carcinogenesis through dysregulation of retinoic acid homeostasis than through oxidation of PAH trans-dihydrodiols. PMID: 18788756
  72. substrate specificity of AKR1B10 is drastically affected by mutation of residue 299 from Cys to Ser PMID: 19028477
  73. The results demonstrated that AKR1B10 consists of 10 exons and 9 introns, stretching approximately 13.8 kb PMID: 19236911
  74. novel role of AKR1B10 in controlling isoprenoid homeostasis that is important in cholesterol synthesis and cell proliferation through salvaging isoprenoid alcohols, as well as its metabolic regulation by endogenous steroids PMID: 19464995
  75. AKR1B10 specifically expressed in the intestine is physiologically important in protecting the host cell against dietary and lipid-derived cytotoxic carbonyls. PMID: 19563777
  76. Data suggest that AKR1B10 affects cell survival through modulating lipid synthesis, mitochondrial function, and oxidative status, as well as carbonyl levels, being an important cell survival protein. PMID: 19643728

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Subcellular Location Lysosome, Secreted
Protein Families Aldo/keto reductase family
Tissue Specificity Found in many tissues. Highly expressed in small intestine, colon and adrenal gland.
Database Links

HGNC: 382

OMIM: 604707

KEGG: hsa:57016

STRING: 9606.ENSP00000352584

UniGene: Hs.116724

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