The product CSB-EL001947HU is a ready-to-use microwell, strip plate ELISA Kit for quantitatively measuring the amount of the Apolipoprotein M (ApoM) in human serum, urine, or tissue homogenates. This assay kit was designed and optimized for signal transduction research use in humans. It has undergone rigorous quality control in multiple parameters, including sensitivity, specificity, precision, linearity, recovery, and inter-batch difference. Refer to the product instructions for more details.
This assay employs the quantitative sandwich enzyme immunoassay technique, in which ApoM in the samples or standards are sandwiched between pre-coated ApoM antibody and Biotin-conjugated ApoM antibody. HRP-avidin is then added into the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of ApoM bound in the initial step. The color development is stopped upon adding the stop solution, and the intensity of the color is measured at 450 nm via a microplate reader. The levels of ApoM in the samples can be determined by referring to the O.D. (optical density) of the samples to the standard curve.
ApoM is a lipocalin with a characteristic beta-barrel structure enclosing a hydrophobic binding pocket that accommodates S1P20. It primarily binds to high-density lipoproteins (HDL) in the serum, promoting pre-β HDL formation and increasing cholesterol efflux from foam cells. Under homeostatic conditions, ApoM plays a potentially protective role in the endothelium. ApoM inhibits lymphopoiesis and neuroinflammation during the adaptive immune response. And high ApoM levels in serum are correlated with chronic obstructive pulmonary disease (COPD).