Human Aquaporin 4,AQP-4 ELISA Kit

Code CSB-E08254h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human AQP4 ELISA Kit was designed for the quantitative measurement of Human AQP4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Target Name aquaporin 4
Alternative Names AQP 4 ELISA Kit; AQP-4 ELISA Kit; AQP4 ELISA Kit; AQP4_HUMAN ELISA Kit; Aquaporin type 4 ELISA Kit; Aquaporin-4 ELISA Kit; Aquaporin4 ELISA Kit; HMIWC 2 ELISA Kit; HMIWC2 ELISA Kit; Mercurial insensitive water channel ELISA Kit; Mercurial-insensitive water channel ELISA Kit; MGC22454 ELISA Kit; MIWC ELISA Kit; WCH 4 ELISA Kit; WCH4 ELISA Kit
Abbreviation AQP4
Uniprot No. P55087
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human AQP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %96
Range %92-103
1:2Average %87
Range %82-93
1:4Average %92
Range %87-96
1:8Average %90
Range %88-93
Recovery
The recovery of human AQP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9890-106
EDTA plasma (n=4)9285-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.568 2.555 2.562 2.472
51.725 1.678 1.702 1.612
2.51.113 1.101 1.107 1.017
1.250.647 0.639 0.643 0.553
0.6250.381 0.356 0.369 0.279
0.3120.218 0.209 0.214 0.124
0.1560.147 0.137 0.142 0.052
00.092 0.088 0.090  
Materials provided
  • A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Human AQP-4 antibody.
  • Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled AQP-4 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
  • One vial Sample Diluent (50 ml/bottle) ---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubating.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Forms a water-specific channel. Plays an important role in brain water homeostasis and in glymphatic solute transport. Required for a normal rate of water exchange across the blood brain interface. Required for normal levels of cerebrospinal fluid influx into the brain cortex and parenchyma along paravascular spaces that surround penetrating arteries, and for normal drainage of interstitial fluid along paravenous drainage pathways. Thereby, it is required for normal clearance of solutes from the brain interstitial fluid, including soluble beta-amyloid peptides derived from APP. Plays a redundant role in urinary water homeostasis and urinary concentrating ability.
Gene References into Functions
  1. AQP4 genetic variation moderates the relationship between sleep and brain Abeta-amyloid burden. PMID: 29479071
  2. our results suggested that miRNA-320a could suppress the aggressive capacity of tumors by targeting AQP4, and that miRNA-320a could serve as a new effective therapeutic target for glioma surgical and therapeutic strategies. PMID: 29484417
  3. This study demonstrated that Concentrations of microparticles expressing GFAP and AQP4 were significantly higher in the traumatic brain injury group compared with healthy controls. PMID: 28972406
  4. Review speculated that the diverse expression of AQP4 isoforms and complement regulatory factors may determine individual susceptibility to disease onset, the expression difference in AQP4 isoforms and complement regulatory factors in different organs may determine the extent of involvement and the severity of the disease. AQP4-IgG-mediated immune injury in peripheral organs may not be rare. PMID: 29141819
  5. Hypothermia-mediated increase in AQP4 surface abundance on human astrocytes, which was blocked using either calmodulin antagonist; TRPV4 antagonist or calcium chelation. A TRPV4 agonist mimicked the effect of hypothermia compared with untreated normothermic astrocytes. Hypothermia increased surface localization of AQP4 in human astrocytes likely through TRPV4 calcium channels and calmodulin activation. PMID: 28925524
  6. AQP4 expression was significantly increased 24 hours after mTBI. mTBI resulted in a significant increase in the cell swelling within 30 min of mTBI, which was significantly reduced in the presence of AZA. PMID: 27623738
  7. the overall results of this study allowed us to provide the first description of the localization of AQP4 in human SGs and indicate an abnormal distribution of AQP4 in SGs from SS patients, which could be responsible for the decreased saliva secretion in these patients. PMID: 28648105
  8. CC genotype of rs72878794 associated with sudden infant death syndrome PMID: 28520217
  9. Aquaporin 4 (AQP-4) are recognized as essential for two unique functions, namely, neurovascular coupling and glymphatic flow, the brain equivalent of systemic lymphatics [Review]. PMID: 28820467
  10. In neuromyelitis optica spectrum disorder-AQP4 patients, gender impacts on disease onset age and site of attack PMID: 27760862
  11. Report the value of spinal cord biopsy in the diagnosis of aquaporin-4 antibody positive neuromyelitis optica spectrum disorder. PMID: 24192218
  12. identified that TMZ might have therapeutic potential for controlling proliferation, invasion of malignant glioma by inhibiting AQP4 expression through activation of p38 signal transduction pathway PMID: 28569417
  13. The purpose of this study was to determine whether or not aquaporin-4 (AQP4) gene mutations are related to the pathogenesis of inflammatory demyelinating diseases in the central nervous system. PMID: 25895050
  14. Comparative molecular dynamics study of neuromyelitis optica-immunoglobulin G binding to AQP4 extracellular domains has been presented. PMID: 28477975
  15. Osmotic water permeabilities of aquaporins AQP4, AQP5, and GlpF from near-equilibrium simulations have been presented. PMID: 28455098
  16. A clear correlation between AQP4 expression and ADCmax values in grade I meningioma was identified. PMID: 27357591
  17. Peritumoral brain edema in patients with meningiomas may depend on AQP4 expression grades and not on tumor grade, tumor volume, Ki-67 expression, and cell count. PMID: 27552812
  18. AQP4 antibodies were higher in neuromyelitis optica Chinese Han patients with circulating auto-antibodies. PMID: 27988051
  19. Retinal nerve fiber layer may be better preserved in MOG-IgG versus AQP4-IgG optic neuritis. PMID: 28125740
  20. AQP4-autoantibody serostatus correlated with poor visual outcome in neuromyelitis optica (Meta-analysis) PMID: 28071581
  21. Data show that aquaporin-4 (AQP4) and myelin-oligodendrocyte glycoprotein (MOG) autoantibodies double positive neuromyelitis optica spectrum disorder (NMOSD) patients had a multiphase disease course with a high annual relapse rate. PMID: 26920678
  22. In this study, altered AQP4 expression was associated with aging brains. PMID: 27893874
  23. Results suggest that pain is an important factor in the quality of life experienced by both AQP4-Ab positive and negative transverse myelitis patients and suggest an even greater association than disability PMID: 27538606
  24. The major findings in this study were (1) a higher AQP4 immunogold labeling density over parenchymal astrocytic membranes in human subjects than in mice, and (2) a lower degree of AQP4 immunogold polarization to perivascular astrocytic endfoot membranes in humans compared with mice. In the human individuals, endfoot AQP4 polarization did not differ between capillary and arteriolar vessel segments. PMID: 28317216
  25. Upregulation of Aquaporin 4 is associated with Astrocytomas. PMID: 27483250
  26. No characteristic MRI brain features were observed in the Malaysian AQP4 seropositive idiopathic inflammatory demyelinating disease patients versus those who are seronegative PMID: 28283103
  27. the T allele of rs2075575 is a risk for AQP4-Ab-positive NMO. PMID: 27012886
  28. A reduction was found in the aquaporin-4 levels in cerebrospinal fluid of patients with intracranial hypertension. PMID: 26853804
  29. Study concludes from direct measurement of deterministic molecular dynamics in conjunction with applied-field metadynamics that the intrinsic electric field within the AQP4 channel points along the +z-axis, such that externally applied static fields in this direction serve to "open" the channel in the selectivity-filter and the asparagine-proline-alanine region. PMID: 27586951
  30. Results indicate that T-cells bearing characteristics of both Th1 and Th17 T-cells and targeting specific immunodominant epitopes of the AQP4 protein might be involved in the pathogenesis of Neuromyelitis optica PMID: 27063619
  31. AQP4 antibody associated optic neuritis tends to involve the posterior optic pathway. PMID: 26163068
  32. Our results suggest that AQP5 but not AQP4 contributes to salivary secretion in patients with Sjogren's syndrome, including those with neuromyelitis optica complicated with Sjogren's syndrome. PMID: 26375433
  33. Administration of E1 + P1 and E1 + P2 decreased the inhibitory effect of E1 on the IL-6 levels and AQP4 protein expression PMID: 26638215
  34. Study shows that the processing of AQP4 by antigen presenting cells in Lewis rats produces a highly encephalitogenic AQP4 epitope (AQP4268-285) PMID: 26530185
  35. The induction of antibodies to an AQP4 epitope in mice immunized with the TAX1BP1-derived peptide suggests that a latent HTLV-1 infection could lead to TAX1BP1 antigen presentation and the production of anti-AQP4 antibodies in human neuromyelitis optica. PMID: 26287441
  36. This study demonstrated that Subcellular localization of Aqp4 was compared between cortical and white matter astrocytes in postmortem specimens of patients with focal ischemic stroke versus controls. PMID: 26419740
  37. Anti-AQP-4 antibodies correlate with active neuromyelitis optica disease activity and animal models PMID: 25913278
  38. AQP4 antibody was highly prevalent (almost 54%) in Egyptian idiopathic inflammatory demyelinating central nervous system patients PMID: 25677878
  39. Study identified two AQP4 genomic variants, rs11661256 and rs1058427, as significant predictors of combined intracerebral hemorrhage and perihematomal edema volume after intracerebral hemorrhage PMID: 26000774
  40. Measurements of the membrane water permeability of MDCK cells expressing human AQP4 mutants provide the first evidencethat the rate of water flow can also be controlled by small movements of single-amino acid side chains lining the water pore. PMID: 26512424
  41. Phosphorylation at serine 276 is necessary for AQP4 translocation in response to changes of tonicity. PMID: 26013827
  42. Case Report: relapsing inappropriate antidiuretic hormone secretion associated with neuromyelitis optica spectrum disorders, in an anti-aquaporin-4 antibody positive 14-year-old girl. PMID: 24866202
  43. Case Report: anti-aquaporin-4 antibody positive neuromyelitis optica spectrum disorder with hypothalamic lesions and sudden onset of sleep. PMID: 25160125
  44. Data suggest that preventing neuromyelitis optica (NMO)-IgG aquaporin-4 (AQP4) binding would represent a strategy for a NMO therapy. PMID: 25839357
  45. The idea is that sudden, disorderly, and excessive neuronal discharges activates NOX2 with O(2)(-) production, leading to lipid peroxidation. The resulting generation of HNE targets AQP4, affecting water and ion balance. PMID: 25460197
  46. This review focuses on the role of AQP4 in ischemic brain edema, and its prospect as a therapeutic target--{REVIEW} PMID: 25306413
  47. analysis of how aquaporin 4 extracellular domains mutations affect restricted binding patterns of pathogenic neuromyelitis optica IgG PMID: 25792738
  48. In conclusion, the present study provides evidence for possible involvement of genetic variations in AQP4 gene in the functional outcome, but not in initial severity or presence of intracranial hemorrhages of patients with TBI. PMID: 24999750
  49. results suggest that AQP4 is internalized and the lysosome is involved in degrading the internalized AQP4 post-intracerebral hemorrhage PMID: 25257965
  50. AQP4 is mainly targeted to the lipid rafts fraction of the plasma membrane and a change of subcellular localization of AQP4 by the treatment with cholesterol-lowering agents significantly reduces the cytotoxic effects of NMO-IgG PMID: 25128605

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Subcellular Location Cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein. Endosome membrane. Cell membrane, sarcolemma; Multi-pass membrane protein. Cell projection.
Protein Families MIP/aquaporin (TC 1.A.8) family
Tissue Specificity Detected in skeletal muscle. Detected in stomach, along the glandular base region of the fundic gland (at protein level). Detected in brain, lung and skeletal muscle, and at much lower levels in heart and ovary.
Database Links

HGNC: 637

OMIM: 600308

KEGG: hsa:361

STRING: 9606.ENSP00000372654

UniGene: Hs.315369

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