Human Aquaporin 4,AQP-4 ELISA Kit

Instructions
Code CSB-E08254h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name aquaporin 4
Alternative Names AQP 4 ELISA Kit; AQP-4 ELISA Kit; AQP4 ELISA Kit; AQP4_HUMAN ELISA Kit; Aquaporin type 4 ELISA Kit; Aquaporin-4 ELISA Kit; Aquaporin4 ELISA Kit; HMIWC 2 ELISA Kit; HMIWC2 ELISA Kit; Mercurial insensitive water channel ELISA Kit; Mercurial-insensitive water channel ELISA Kit; MGC22454 ELISA Kit; MIWC ELISA Kit; WCH 4 ELISA Kit; WCH4 ELISA Kit
Abbreviation AQP4
Uniprot No. P55087
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human AQP-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %96
Range %92-103
1:2Average %87
Range %82-93
1:4Average %92
Range %87-96
1:8Average %90
Range %88-93
Recovery
The recovery of human AQP-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9890-106
EDTA plasma (n=4)9285-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.568 2.555 2.562 2.472
51.725 1.678 1.702 1.612
2.51.113 1.101 1.107 1.017
1.250.647 0.639 0.643 0.553
0.6250.381 0.356 0.369 0.279
0.3120.218 0.209 0.214 0.124
0.1560.147 0.137 0.142 0.052
00.092 0.088 0.090  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
Gene References into Functions
  1. AQP4 genetic variation moderates the relationship between sleep and brain Abeta-amyloid burden. PMID: 29479071
  2. our results suggested that miRNA-320a could suppress the aggressive capacity of tumors by targeting AQP4, and that miRNA-320a could serve as a new effective therapeutic target for glioma surgical and therapeutic strategies. PMID: 29484417
  3. This study demonstrated that Concentrations of microparticles expressing GFAP and AQP4 were significantly higher in the traumatic brain injury group compared with healthy controls. PMID: 28972406
  4. Review speculated that the diverse expression of AQP4 isoforms and complement regulatory factors may determine individual susceptibility to disease onset, the expression difference in AQP4 isoforms and complement regulatory factors in different organs may determine the extent of involvement and the severity of the disease. AQP4-IgG-mediated immune injury in peripheral organs may not be rare. PMID: 29141819
  5. Hypothermia-mediated increase in AQP4 surface abundance on human astrocytes, which was blocked using either calmodulin antagonist; TRPV4 antagonist or calcium chelation. A TRPV4 agonist mimicked the effect of hypothermia compared with untreated normothermic astrocytes. Hypothermia increased surface localization of AQP4 in human astrocytes likely through TRPV4 calcium channels and calmodulin activation. PMID: 28925524
  6. AQP4 expression was significantly increased 24 hours after mTBI. mTBI resulted in a significant increase in the cell swelling within 30 min of mTBI, which was significantly reduced in the presence of AZA. PMID: 27623738
  7. the overall results of this study allowed us to provide the first description of the localization of AQP4 in human SGs and indicate an abnormal distribution of AQP4 in SGs from SS patients, which could be responsible for the decreased saliva secretion in these patients. PMID: 28648105
  8. CC genotype of rs72878794 associated with sudden infant death syndrome PMID: 28520217
  9. Aquaporin 4 (AQP-4) are recognized as essential for two unique functions, namely, neurovascular coupling and glymphatic flow, the brain equivalent of systemic lymphatics [Review]. PMID: 28820467
  10. In neuromyelitis optica spectrum disorder-AQP4 patients, gender impacts on disease onset age and site of attack PMID: 27760862
  11. Report the value of spinal cord biopsy in the diagnosis of aquaporin-4 antibody positive neuromyelitis optica spectrum disorder. PMID: 24192218
  12. identified that TMZ might have therapeutic potential for controlling proliferation, invasion of malignant glioma by inhibiting AQP4 expression through activation of p38 signal transduction pathway PMID: 28569417
  13. The purpose of this study was to determine whether or not aquaporin-4 (AQP4) gene mutations are related to the pathogenesis of inflammatory demyelinating diseases in the central nervous system. PMID: 25895050
  14. Comparative molecular dynamics study of neuromyelitis optica-immunoglobulin G binding to AQP4 extracellular domains has been presented. PMID: 28477975
  15. Osmotic water permeabilities of aquaporins AQP4, AQP5, and GlpF from near-equilibrium simulations have been presented. PMID: 28455098
  16. A clear correlation between AQP4 expression and ADCmax values in grade I meningioma was identified. PMID: 27357591
  17. Peritumoral brain edema in patients with meningiomas may depend on AQP4 expression grades and not on tumor grade, tumor volume, Ki-67 expression, and cell count. PMID: 27552812
  18. AQP4 antibodies were higher in neuromyelitis optica Chinese Han patients with circulating auto-antibodies. PMID: 27988051
  19. Retinal nerve fiber layer may be better preserved in MOG-IgG versus AQP4-IgG optic neuritis. PMID: 28125740
  20. AQP4-autoantibody serostatus correlated with poor visual outcome in neuromyelitis optica (Meta-analysis) PMID: 28071581
  21. Data show that aquaporin-4 (AQP4) and myelin-oligodendrocyte glycoprotein (MOG) autoantibodies double positive neuromyelitis optica spectrum disorder (NMOSD) patients had a multiphase disease course with a high annual relapse rate. PMID: 26920678
  22. In this study, altered AQP4 expression was associated with aging brains. PMID: 27893874
  23. Results suggest that pain is an important factor in the quality of life experienced by both AQP4-Ab positive and negative transverse myelitis patients and suggest an even greater association than disability PMID: 27538606
  24. The major findings in this study were (1) a higher AQP4 immunogold labeling density over parenchymal astrocytic membranes in human subjects than in mice, and (2) a lower degree of AQP4 immunogold polarization to perivascular astrocytic endfoot membranes in humans compared with mice. In the human individuals, endfoot AQP4 polarization did not differ between capillary and arteriolar vessel segments. PMID: 28317216
  25. Upregulation of Aquaporin 4 is associated with Astrocytomas. PMID: 27483250
  26. No characteristic MRI brain features were observed in the Malaysian AQP4 seropositive idiopathic inflammatory demyelinating disease patients versus those who are seronegative PMID: 28283103
  27. the T allele of rs2075575 is a risk for AQP4-Ab-positive NMO. PMID: 27012886
  28. A reduction was found in the aquaporin-4 levels in cerebrospinal fluid of patients with intracranial hypertension. PMID: 26853804
  29. Study concludes from direct measurement of deterministic molecular dynamics in conjunction with applied-field metadynamics that the intrinsic electric field within the AQP4 channel points along the +z-axis, such that externally applied static fields in this direction serve to "open" the channel in the selectivity-filter and the asparagine-proline-alanine region. PMID: 27586951
  30. Results indicate that T-cells bearing characteristics of both Th1 and Th17 T-cells and targeting specific immunodominant epitopes of the AQP4 protein might be involved in the pathogenesis of Neuromyelitis optica PMID: 27063619
  31. AQP4 antibody associated optic neuritis tends to involve the posterior optic pathway. PMID: 26163068
  32. Our results suggest that AQP5 but not AQP4 contributes to salivary secretion in patients with Sjogren's syndrome, including those with neuromyelitis optica complicated with Sjogren's syndrome. PMID: 26375433
  33. Administration of E1 + P1 and E1 + P2 decreased the inhibitory effect of E1 on the IL-6 levels and AQP4 protein expression PMID: 26638215
  34. Study shows that the processing of AQP4 by antigen presenting cells in Lewis rats produces a highly encephalitogenic AQP4 epitope (AQP4268-285) PMID: 26530185
  35. The induction of antibodies to an AQP4 epitope in mice immunized with the TAX1BP1-derived peptide suggests that a latent HTLV-1 infection could lead to TAX1BP1 antigen presentation and the production of anti-AQP4 antibodies in human neuromyelitis optica. PMID: 26287441
  36. This study demonstrated that Subcellular localization of Aqp4 was compared between cortical and white matter astrocytes in postmortem specimens of patients with focal ischemic stroke versus controls. PMID: 26419740
  37. Anti-AQP-4 antibodies correlate with active neuromyelitis optica disease activity and animal models PMID: 25913278
  38. AQP4 antibody was highly prevalent (almost 54%) in Egyptian idiopathic inflammatory demyelinating central nervous system patients PMID: 25677878
  39. Study identified two AQP4 genomic variants, rs11661256 and rs1058427, as significant predictors of combined intracerebral hemorrhage and perihematomal edema volume after intracerebral hemorrhage PMID: 26000774
  40. Measurements of the membrane water permeability of MDCK cells expressing human AQP4 mutants provide the first evidencethat the rate of water flow can also be controlled by small movements of single-amino acid side chains lining the water pore. PMID: 26512424
  41. Phosphorylation at serine 276 is necessary for AQP4 translocation in response to changes of tonicity. PMID: 26013827
  42. Case Report: relapsing inappropriate antidiuretic hormone secretion associated with neuromyelitis optica spectrum disorders, in an anti-aquaporin-4 antibody positive 14-year-old girl. PMID: 24866202
  43. Case Report: anti-aquaporin-4 antibody positive neuromyelitis optica spectrum disorder with hypothalamic lesions and sudden onset of sleep. PMID: 25160125
  44. Data suggest that preventing neuromyelitis optica (NMO)-IgG aquaporin-4 (AQP4) binding would represent a strategy for a NMO therapy. PMID: 25839357
  45. The idea is that sudden, disorderly, and excessive neuronal discharges activates NOX2 with O(2)(-) production, leading to lipid peroxidation. The resulting generation of HNE targets AQP4, affecting water and ion balance. PMID: 25460197
  46. This review focuses on the role of AQP4 in ischemic brain edema, and its prospect as a therapeutic target--{REVIEW} PMID: 25306413
  47. analysis of how aquaporin 4 extracellular domains mutations affect restricted binding patterns of pathogenic neuromyelitis optica IgG PMID: 25792738
  48. In conclusion, the present study provides evidence for possible involvement of genetic variations in AQP4 gene in the functional outcome, but not in initial severity or presence of intracranial hemorrhages of patients with TBI. PMID: 24999750
  49. results suggest that AQP4 is internalized and the lysosome is involved in degrading the internalized AQP4 post-intracerebral hemorrhage PMID: 25257965
  50. AQP4 is mainly targeted to the lipid rafts fraction of the plasma membrane and a change of subcellular localization of AQP4 by the treatment with cholesterol-lowering agents significantly reduces the cytotoxic effects of NMO-IgG PMID: 25128605
  51. Flow cytometry and bridging ELISA are useful for detecting and quantifying anti-AQP4 antibodies in neuromyelitis optica. PMID: 22526930
  52. A patient with neuromyelitis optica had decreased AQP4 antibody levels with development of brain lesions. PMID: 25668569
  53. Our results suggest that hyperosmotic stress markedly reduces AQP4 expression possibly through a proteasome ubiquitinylation-independent pathway. PMID: 24888368
  54. The expression of AQP4-Delta4 may represent a new regulatory mechanism through which the cell-surface expression and therefore the activity of AQP4 can be physiologically modulated. PMID: 24356448
  55. results indicate that AQP-4 genetic variations and haplotypes might contribute to the risk factors for leukoaraiosis. PMID: 24582793
  56. A pivotal role for AQP4 transmembrane regions in epitope assembly in neuromyelitis optica. PMID: 25239624
  57. Case Reports: diagnosis of neuromyelitis optica spectrum disorders supported by their AQP4-seropositivity. PMID: 24029914
  58. Polymorphisms in the coding region of AQP4 are unlikely to confer neuromyelitis optica susceptibility; the 3' UTR of this gene presents several polymorphic sites that may affect risk in the Chinese. PMID: 25096173
  59. In demyelinating disease, major lesions in the three anti-contactin-associated protein 2 Ab-positive subjects were infratentorial, including one co-carrying anti-AQP4 Abs. PMID: 25027061
  60. Variations in the AQP4 gene seem of limited significance as predisposing factors in Caucasian SIDS infants PMID: 24727946
  61. The expression of AQP4 mRNA in Muller cells was increased by VEGF. PMID: 22886979
  62. These results suggest that AQP4 functions as the modulator of synaptic plasticity and memory, and chronic Cef treatment rescues hippocampal memory deficit induced by AQP4 knockout. PMID: 23973312
  63. Increased AQP4 expression could be an independent risk factor in neuromyelitis optica and other forms of CNS inflammation. PMID: 24182872
  64. Water self-diffusion and the dipolar response of the selectivity filter within human aquaporin 4 have been studied using molecular dynamics (MD) simulations in the absence and presence of pulses of external static and alternating electric fields. PMID: 24289379
  65. Aquaporin-4 autoantibodies in Neuromyelitis Optica: AQP4 isoform-dependent sensitivity and specificity. PMID: 24260168
  66. Demonstration of AQP4 immunoreactivity in this patient's small bowel endocrine tumor supports a paraneoplastic etiology for neuromyelitis optica spectrum disorder (NMOSD). PMID: 24733266
  67. [review] The discovery of autoantibodies to AQP-4, the most abundant water channel in the central nervous system, has led to the recognition of neuromyelitis optica as an autoimmune condition and as a disease entity in its own right. PMID: 24388217
  68. No significant associations between AQP4 SNPs/haplotypes and development of IDD. PMID: 24361961
  69. 5-aminolevulinic acid fluorescing tissue showed higher AQP4 expression compared to normal brain tissue PMID: 23915916
  70. Patients with neuromyelitis optica spectrum disorders with MOG antibodies have distinct clinical features, fewer attacks, and better recovery than patients with AQP4 antibodies or patients seronegative for both antibodies. PMID: 24415568
  71. Knockdown a water channel protein, aquaporin-4, induced glioblastoma cell apoptosis. PMID: 23950863
  72. In a sample of patients with multiple sclerosis, aquaporin-4 antibodies were detected in CSF but not serum. PMID: 23232103
  73. Based on these initial results, we believe [11C]TGN-020 PET will be valuable in determining the role of AQP-4 in disease progression, and for the clinical assessment of water homeostasis under various settings. PMID: 22817997
  74. Serological tests using recombinant AQP4 antigen are significantly more sensitive than tissue-based immunofluorescence for detecting AQP4-immunoglobulin G (IgG). PMID: 23997151
  75. conclude that high AQP4 antibody levels are associated with the occurrence of ON, but that the antibody levels themselves are not closely correlated with the onset of relapse PMID: 23456674
  76. Aquaporin-4 antibody-positive patients should be included in the neuromyelitis optica spectrum even if they don't fulfill diagnostic criteria. PMID: 23677744
  77. Patients diagnosed with AQP4-antibody-negative neuromyelitis optica express specific demographic and disease-related features. PMID: 23658379
  78. Presence of AQP4 antibodies is associated with the conversion rate from transverse myelitis to neuromyelitis optica. PMID: 22829325
  79. We compared human AQP4 to plant and bacterial proteins to investigate the occurrence of significantly similar structures and sequences. PMID: 23664693
  80. We examined whether Multiple Sclerosis (MS) patients recognize antigenic epitopes on AQP4 that may define distinct disease subsets. PMID: 23673145
  81. Neuromyelitis optica is a demyelinating disease characterized by a disease-specific autoantibody designated as NMO-IgG that specifically recognizes aquaporin-4, and the binding of NMO-IgG to AQP4 causes the progress of the disease. PMID: 23746426
  82. Compared with brain astrocytes, Muller cells (a class of retinal macroglia) exhibit higher levels of AQP4. PMID: 22987438
  83. neuromyelitis optica-IgG binds most avidly to plasma membrane surface AQP4 epitopes formed by loop interactions within tetramers and by intermolecular interactions within high order structures. PMID: 22906356
  84. Astrocytes express aquaporin 4, the water channel protein, involved in water homeostasis and edema formation PMID: 23270503
  85. Our genetic case-control study did not indicate an association between AQP4 gene variants and Idiopathic intracranial hypertension. PMID: 21914143
  86. AQP4 orthogonal arrays of particles were imaged with ~20 nm spatial precision by photoactivation localization microscopy. PMID: 22718347
  87. Structural features of aquaporin 4 supporting the formation of arrays and junctions in biomembranes PMID: 22554469
  88. Genetic variation in AQP4 may account for the susceptibility to AQP4-Ab-positive neuromyelitis optica and its spectrum disorders in Southern Han Chinese population. PMID: 23116879
  89. Both cognitive impairment and substantial cortical neuronal loss are demonstrated with unique AQP4 dynamics in astrocytes from neuromyelitis optica patients. PMID: 23378324
  90. The subcellular co-localisation of K(ir)4.1 and AQP4 in the supporting cells of the cochlea described in this study resembles that of the astroglia of the central nervous system and the glial Mueller cells in the retina. PMID: 22802001
  91. Reduced expression of cell surface AQP-4 is characteristic of glioblastoma multiforme patients without seizures, likely attributable to a posttranslational mechanism. PMID: 22714714
  92. The presence and severity of CCVSI in multiple sclerosis correlate with disease status but has no or very limited association with clinical disability. PMID: 22540328
  93. AQP4 expression associates with spinal cord lesions in two cases of neuromyelitis optica. PMID: 23054238
  94. An autoimmune response against Aqp4 in neuromyelitis optica may be triggered by infection-induced cross-immunoreactivity and presents a new perspective on the pathogenesis of this disease. PMID: 23008451
  95. AQP4 gene expression was significantly higher in miRNA-203 down-regulated asthmatic airway epithelia. PMID: 22679274
  96. The frequency of anti-AQP4 in recurrentMacute transverse myelitis was 26.9%, increasing to 41.2% among extensive transverse myelitis PMID: 22925751
  97. NMO-IgG against aquaporin-4 (AQP4) is a specific marker for neuromyelitis optica (NMO). We evaluated the performance of different NMO-IgG detecting methods.ELISA-AQP4 may be more sensitive and specific when confirmed by IIF-AQP4. PMID: 22628234
  98. This review showed that the potential roles of the glial water channel aquaporin-4 (AQP4) in modulation of brain excitability and in epilepsy. PMID: 22378467
  99. Our findings suggest differential expression patterns of MMP-9 and AQP4 in different grades of gliomas PMID: 22773464
  100. AQP4 mRNA expression is downregulated by hypoxia and ischemia. PMID: 22777185

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Subcellular Location Membrane, Multi-pass membrane protein
Protein Families MIP/aquaporin (TC 1.A.8) family
Tissue Specificity Brain - muscle >> heart, kidney, lung, and trachea.
Database Links

HGNC: 637

OMIM: 600308

KEGG: hsa:361

STRING: 9606.ENSP00000372654

UniGene: Hs.315369

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