Human Collagen Type Ⅲ,Col Ⅲ ELISA KIT

Code CSB-E04799h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now
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Product Details

Target Name
Collagen Type Ⅲ,Col Ⅲ
Alternative Names
Collagen Type 3,Col 3,Collagen Type III,Col III
Abbreviation
Col Ⅲ
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
12.5 ng/mL-800 ng/mL
Sensitivity
3.12 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 86-96  
1:2 Average % 102  
Range % 97-105  
1:4 Average % 92  
Range % 85-98  
1:8 Average % 97  
Range % 91-102  
Recovery
The recovery of human Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 86 80-94  
EDTA plasma (n=4) 97 92-103  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
800 2.850 2.963 2.907 2.778  
400 2.255 2.203 2.229 2.100  
200 1.279 1.256 1.268 1.139  
100 0.659 0.655 0.657 0.528  
50 0.438 0.475 0.457 0.328  
25 0.256 0.242 0.249 0.120  
12.5 0.184 0.172 0.178 0.049  
0 0.126 0.132 0.129    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days
Description

This Human Col Ⅲ ELISA Kit was designed for the quantitative measurement of Human Col Ⅲ protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 ng/mL-800 ng/mL and the sensitivity is 3.12 ng/mL .

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