Human Cross-linked Carboxy-terminal telopeptide of type Ⅰ collagen, CTX-I ELISA Kit

Code CSB-E11224h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details


This Human CTX-I ELISA Kit was designed for the quantitative measurement of Human CTX-I protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

Target Name Cross-linked Carboxy-terminal telopeptide of type Ⅰ collagen, CTX-I
Alternative Names Cross-linked Carboxy-terminal telopeptide of type 1 collagen, CTX-1,Cross-linked Carboxy-terminal telopeptide of type I collagen, CTX-I
Abbreviation CTX-I
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human CTX-I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 83-95  
1:2 Average % 95  
Range % 91-98  
1:4 Average % 97  
Range % 90-103  
1:8 Average % 96  
Range % 91-101  
The recovery of human CTX-I spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 85 81-92  
EDTA plasma (n=4) 96 93-106  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
40 2.995 2.960 2.978 2.838  
20 2.557 2.570 2.564 2.424  
10 1.666 1.765 1.716 1.576  
5 0.839 0.886 0.863 0.723  
2.5 0.491 0.524 0.508 0.368  
1.25 0.263 0.251 0.257 0.117  
0.625 0.201 0.212 0.207 0.067  
0 0.138 0.142 0.140    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


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Please provide the following information regarding Human Cross-linked Carboxy-terminal telopeptide of type I collagen, CTX-I ELISA Kit CSB-E11224h
a. Is the capture antibody a monoclonal or polyclonal?
b. What is the host of the capture antibody?
c. Is the detection antibody a monoclonal or polyclonal?
d. What is the host of the detection antibody?
e. B208. What’s is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
f. If recombinant protein, is it full length or partial?
g. What is the species of the standard– human or something else?

Thanks for your inquiry.
Capture antibody is mouse monoclonal antibody and detection antibody is goat polyclonal antibody.
Standard is synthetic peptide.
Pls let me know if you have any further questions. Thank you.
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