Target Name
lectin, galactoside-binding, soluble, 3 binding protein
Alternative Names
90K ELISA Kit; 90K MAC 2 BP ELISA Kit; Basement membrane autoantigen p105 ELISA Kit; BTBD17B ELISA Kit; Galectin 3 binding protein ELISA Kit; Galectin-3-binding protein ELISA Kit; L3 antigen ELISA Kit; Lectin galactoside binding soluble 3 binding protein ELISA Kit; Lectin galactoside-binding soluble 3-binding protein ELISA Kit; LG3BP_HUMAN ELISA Kit; LGALS3BP ELISA Kit; M2BP ELISA Kit; Mac 2 binding protein ELISA Kit; Mac 2 BP ELISA Kit; Mac-2 BP ELISA Kit; Mac-2-binding protein ELISA Kit; Mac2 BP ELISA Kit; MAC2BP ELISA Kit; Serum protein 90K ELISA Kit; TANGO10B ELISA Kit; Transport and golgi organization 10 homolog B ELISA Kit; Tumor associated antigen 90K ELISA Kit; Tumor-associated antigen 90K ELISA Kit
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
1.56 ng/mL-100 ng/mL
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Precision
Intra-assay Precision (Precision within an assay): CV%<8% |
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Three samples of known concentration were tested twenty times on one plate to assess. |
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Inter-assay Precision (Precision between assays): CV%<10% |
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Three samples of known concentration were tested in twenty assays to assess. |
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Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human LGALS3BP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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1:20 |
Average % |
91 |
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Range % |
84-98 |
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1:40 |
Average % |
90 |
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Range % |
85-95 |
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1:60 |
Average % |
97 |
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Range % |
92-102 |
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1:160 |
Average % |
102 |
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Range % |
96-108 |
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Recovery
The recovery of human LGALS3BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
Sample Type |
Average % Recovery |
Range |
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Serum (n=5) |
100 |
95-106 |
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EDTA plasma (n=4) |
90 |
85-95 |
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Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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100 |
2.620 |
2.645 |
2.633 |
2.541 |
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50 |
2.265 |
2.366 |
2.316 |
2.224 |
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25 |
1.628 |
1.673 |
1.651 |
1.559 |
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12.5 |
0.921 |
0.855 |
0.888 |
0.796 |
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6.25 |
0.571 |
0.589 |
0.580 |
0.488 |
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3.12 |
0.333 |
0.355 |
0.344 |
0.252 |
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1.56 |
0.190 |
0.188 |
0.189 |
0.097 |
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0 |
0.093 |
0.090 |
0.092 |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description
This Human LGALS3BP ELISA Kit was designed for the quantitative measurement of Human LGALS3BP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL.