Human H-Cadherin,H-Cad/CDH13 ELISA Kit

Code CSB-E13817h
Size 96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name cadherin 13, H-cadherin (heart)
Alternative Names CAD13_HUMAN ELISA Kit; Cadherin-13 ELISA Kit; CDH13 ELISA Kit; CDHH ELISA Kit; H-cadherin ELISA Kit; Heart cadherin ELISA Kit; P105 ELISA Kit; T cad ELISA Kit; T Cadherin ELISA Kit; T-cad ELISA Kit; T-cadherin ELISA Kit; Truncated cadherin ELISA Kit
Abbreviation CDH13
Uniprot No. P55290
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.25 ng/mL-80 ng/mL
Sensitivity 0.312 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human H-Cad/CDH13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 86-97  
1:2 Average % 92  
Range % 89-96  
1:4 Average % 102  
Range % 99-105  
1:8 Average % 94  
Range % 87-99  
Recovery
The recovery of human H-Cad/CDH13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 93-102  
EDTA plasma (n=4) 102 97-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
80 2.367 2.235 2.301 2.169  
40 1.756 1.816 1.786 1.654  
20 1.286 1.224 1.255 1.123  
10 0.815 0.801 0.808 0.676  
5 0.464 0.453 0.459 0.327  
2.5 0.323 0.312 0.318 0.186  
1.25 0.228 0.235 0.232 0.100  
0 0.133 0.131 0.132    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May act as a negative regulator of neural cell growth.
Gene References into Functions
  1. In this young Chinese population, CDH13 rs4783244 represents a key locus for cardiac structure, and confers stronger cardio-protection in longer sleep duration when contrasted with short sleep duration PMID: 29903569
  2. Polymorphism in CDH13 is associated with nephropathy in subjects with type 1 diabetes. PMID: 28499019
  3. The single nucleotide polymorphisms (SNPs) rs12596316AG genotype of the T-cadherin (CDH13) gene is associated with the susceptibility to metabolic syndrome (MS) among ethnic Han Chinese. PMID: 29419874
  4. the data suggests that the CDH13 T > A (rs11646213) polymorphism is associated with decreased risk of developing hypertension in the Mexican population PMID: 27682011
  5. nine ADHD candidate single nucleotide polymorphisms (SNPs) in seven genes were tested for association with PD in 5333 cases and 12,019 healthy controls. No significant association was observed. PMID: 28176268
  6. CDH13 genetic polymorphisms are associated with adiponectin levels and ischemic stroke. PMID: 28245897
  7. Study evaluated the associations between 6 SNPs in CDH13 and type 2 diabetes mellitus (T2DM) in a Han Chinese population. Results showed that the rs12596316 AG genotype was a risk genotype for the development of T2DM in the overdominant inheritance model; rs11646213, rs3865188, rs12444338, rs12051272, and rs7195409 had no observed associations with T2DM in terms of alleles, genotypes, and the various inheritance models. PMID: 28562572
  8. The methylation status of CDH13 promoter was strongly associated with breast cancer risk. However, CDH13 promoter methylation was not significantly related to the OS and DFS of breast cancer and may have limited prognostic value for breast cancer patients PMID: 27153114
  9. Studied the associations between genetic variants of CDH13 and type 2 diabetes (T2D), and its related parameters, in a Caucasian population. PMID: 27289142
  10. Compared with adjacent normal tissues, the methylation frequencies of WIF-1, RASSF1A, and CDH13 genes were significantly higher but the mRNA levels of these 3 genes were significantly lower in EC tissues. The survival rates of patients with WIF-1, RASSF1A, and CDH13 methylations were significantly lower than those of patients without methylation PMID: 27506957
  11. higher promoter methylation in colorectal cancer than in premalignant, normal, adjacent tissues; highest promoter methylation in poorly differentiated colorectal cancer PMID: 28121942
  12. CDH13 genetic variants determine Chinese individuals' susceptibility to chronic obstructive pulmonary disease (COPD) and thus are efficient genetic biomarkers for early detection of COPD. PMID: 26806298
  13. Strong Functional Association of adipor2 and cdh13 with adipoq PMID: 25388841
  14. CDH13 Polymorphisms are Associated with Adiponectin Levels and Metabolic Syndrome Traits Independently of Visceral Fat Mass. PMID: 26423718
  15. CDH13 locus variants and adiponectin levels are associated with circulating levels of cellular adhesion molecules and adiposity status in a differential manner that interacts with sex PMID: 26600672
  16. This study finding significant excess of rare nonsynonymous variants exclusive to European Americans smokers in CHD13. PMID: 25450229
  17. Data demonstrate for the first time that SHP1 methylation has high specificity for diagnosis of endometrial carcinoma, while CDH13 promoter methylation plays a role in the earlier stage. PMID: 26597461
  18. The present study identified a new genetic factor for CRC risk and an interaction between CDH13 and APN in CRC risk. These genetic factors may be useful for predicting CRC risk. PMID: 26362652
  19. The downregulation of T-cadherin may contribute to gastric cancer progression, representing a useful biomarker for predicting the biological behavior and prognosis of gastric cancer. PMID: 25847144
  20. Finnish prisoners, revealed that a monoamine oxidase A (MAOA) low-activity genotype (contributing to low dopamine turnover rate) as well as the CDH13 gene (coding for neuronal membrane adhesion protein) are associated with extremely violent behavior PMID: 25349169
  21. The genetic polymorphisms at the CDH13 locus independently affect the adiponectin levels, whereas the adiponectin levels exhibit a suppressive effect on the association between CDH13 locus variants and various metabolic phenotypes and metabolic syndrome PMID: 25875811
  22. The CDH13 rs11150556 CC genotype was associated with more hyperactive/impulsive symptoms in attention/deficit hyperactivity disorder. PMID: 25739828
  23. CDH13 promoter methylation is associated with lung cancer. PMID: 25735345
  24. Aberrant promoter methylation of the cadherin 13 gene in serum is associated with prostate cancer. PMID: 25015764
  25. Our results suggested that negative T-cadherin expression has a worse prognosis in patients with axillary lymph node-positive breast cancer. PMID: 25677746
  26. down-regulation of adiponectin receptors (AdipoR1, R2, and T-cadherin) in osteoarthritic chondrocytes PMID: 24888493
  27. CDH13 DNA is methylated in only ten percent of cervical cancer patients. PMID: 22942707
  28. High frequency of CDH13 hypermethylation is associated with melanoma brain metastases. PMID: 24968695
  29. CDH13 methylation is a frequent event in non-muscle invasive bladder cancer. PMID: 25196672
  30. Several SNPs in the CDH13 promoter region are significantly associated with the level of DNA methylation at nearby CpG sites. PMID: 25543204
  31. T-cadherin regulates prostate cancer cell behavior by tuning the balance in EGFR/IGF-1R activity and enhancing the impact of IGF-1R PMID: 25381040
  32. High methylation level of CDH13 gene promoter region is associated with low drug sensitivity of non-small cell lung cancer. PMID: 24998565
  33. T-cadherin exhibited a state of insulin insensitivity as evidenced by attenuation of the ability of insulin to stimulate Akt/mTOR axis signaling, phosphorylation of MLC20 and MYPT1 PMID: 24815187
  34. Adiponectin/T-cadherin and apelin/APJ expression patterns were found to be inversely associated with human aortic and coronary atherosclerosis. PMID: 24675084
  35. CDH13 genotype may be a factor that affects not only the plasma level of HMWA but also the prognostic significance of HMWA. PMID: 24041676
  36. A non significant trend for higher levels of total adiponectin was observed in adult attention deficit hyperactivity disorder patients carrying CDH13 missense mutations compared to patients with wild type CDH13. PMID: 24559850
  37. The SNPs in ADP-ribosylation factor-like protein 15 (ARL15) and the T-cadherin (CDH13) genes did not exhibit significant association with individual metabolic traits in the T2DM and NDM groups. PMID: 24688318
  38. The SNPs most strongly associated in the single-marker analysis of the combined Danish samples were rs4757144 in ARNTL (P=3.78 x 10-6) and rs8057927 in CDH13. PMID: 23358160
  39. High CDH-13 expression is associated with melanoma. PMID: 23625515
  40. The results provide evidence that CDH13 variants are associated with metabolic traits and carotid atherosclerosis in Koreans. PMID: 24142632
  41. genome-wide association study in populations in Minnesota and Utah: Data suggest that SNPs on chromosome 12 and in CDH13 (H-cadherin) are associated with baseline circulating levels of adiponectin (and response of adiponectin levels to fenofibrate). PMID: 23149075
  42. high expressionsin uterine leiomyoma, associated with color Doppler flow imaging PMID: 23463326
  43. the frequency of coding CDH13 variants in adult attention deficit/hyperactivity disorder PMID: 23936508
  44. Cadherin 13 might play a role in the protoporphyrin (Pp)IX accumulation pathway and act as a negative regulator of 5-ALA-induced fluorescence in glioma cells. PMID: 24010971
  45. CDH13 variants strongly influence plasma total and high molecular weight adiponectin levels in East Asian populations but appear to alter adiponectin sensitivity, resulting in better metabolic health than expected based on circulating adiponectin levels. PMID: 24009259
  46. T-cadherin translocation to cell-cell contacts is sensitive to the activity status of EGFR, requires lipid raft domain integrity and actin filament polymerization, and crucial intracellular signalling mediators include Rac1 and p38MAPK. PMID: 23411345
  47. Downregulated expression of CDH13 is associated with increased invasion of bladder transitional cell carcinoma PMID: 23235385
  48. Data indicate that T-cadherin-silencing promotes experimental metastasis of squamous cell carcinoma (SCC). PMID: 23369463
  49. The present study investigated transcriptional regulation of CDH13 in melanoma. Found an inverse correlation between BRN2 and T-cadherin protein and transcript expression in melanoma. PMID: 23069940
  50. This study identifying loci for aADHD and led to the identification of CHD13 as novel genes associated with ADHD across the lifespan. PMID: 22105624

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Subcellular Location Cell membrane; Lipid-anchor, GPI-anchor.
Tissue Specificity Highly expressed in heart. In the CNS, expressed in cerebral cortex, medulla, hippocampus, amygdala, thalamus and substantia nigra. No expression detected in cerebellum or spinal cord.
Database Links

HGNC: 1753

OMIM: 601364

KEGG: hsa:1012

STRING: 9606.ENSP00000408632

UniGene: Hs.654386

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