Human Myostatin,MSTN ELISA Kit

Instructions
Code CSB-E11300h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name myostatin
Alternative Names Cmpt ELISA Kit; GDF-8 ELISA Kit; GDF8_HUMAN ELISA Kit; Growth/differentiation factor 8 ELISA Kit; MSLHP ELISA Kit; MSTN ELISA Kit; MSTN myostatin ELISA Kit; Myostatin ELISA Kit; Myostatin Propeptide ELISA Kit
Abbreviation MSTN
Uniprot No. O14793
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 ng/mL-20 ng/mL
Sensitivity 0.312 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 92-99  
1:2 Average % 94  
Range % 91-98  
1:4 Average % 101  
Range % 97-105  
1:8 Average % 86  
Range % 82-90  
Recovery
The recovery of human MSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 87-97  
EDTA plasma (n=4) 86 82-91  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 1.851 1.992 1.922 1.878  
10 1.058 1.136 1.097 1.053  
4.375 0.502 0.524 0.513 0.469  
1.875 0.268 0.255 0.262 0.218  
0.625 0.116 0.119 0.118 0.074  
0 0.043 0.045 0.044    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Acts specifically as a negative regulator of skeletal muscle growth.
Gene References into Functions
  1. Mstn deficiency-induced apoptosis took place along with generation of reactive oxygen species and elevated fatty acid oxidation, which may play a role in triggering mitochondrial membrane depolarization, the release of cytochrome c, and caspase activation. PMID: 30241032
  2. the maturation and secretion of myostatin precursor MstnPP and its metabolites in a human muscle cell line, was investigated. PMID: 29546591
  3. Acute high-intensity interval exercise decreased irisin levels and increased myostatin levels. PMID: 29558345
  4. Autosomal Dominant Polycystic Kidney Disease patients with moderately preserved renal function have higher levels of FasL, myostatin and urine TGF-beta1 than controls PMID: 29794429
  5. Tolloid cleavage activates latent GDF8 by destabilizing specific prodomain-growth factor interfaces and primes the growth factor for release from the prodomain. PMID: 29343545
  6. Myostatin pathway is down-regulated in the neuromuscular diseases. PMID: 29192144
  7. the prodomain:GDF8 complex can exist in a fully latent state and an activated or "triggered" state where the prodomain remains in complex with the mature domain PMID: 29348202
  8. Higher serum myostatin levels correlated with muscle mass loss, hyperammonemia, and impaired protein synthesis, as reflected by lower serum albumin levels and lower branched-chain amino acid to tyrosine ratio levels. High serum myostatin levels were also associated with a reduced OS rate in LC patients. PMID: 28627027
  9. Study showed the expression of myostatin in healthy endometrium and a higher expression in endometriosis and endometrial cancer, suggesting myostatin involvement in human endometrial physiology and related pathologies. PMID: 28345488
  10. Studied levels of myostatin in both serum and synovial fluid in patients with knee osteoarthritis and found both correlated with severity of knee osteoarthritis. PMID: 27878995
  11. Myostatin (and Smad2) were significantly up-regulated in the failing heart of female patients, but not male patients. PMID: 28465115
  12. The Growth Differentiation Factor 11 (GDF11) and Myostatin (MSTN) in tissue specific aging. PMID: 28472635
  13. MSTN 153Arg(R) polymorphism is associated with long distance running success. PMID: 28007336
  14. GDF8 promotes ovarian cancer cell migration via ALK4/5-SMAD2/3-E-cadherin signaling. PMID: 27481097
  15. Results demonstrat that GDF8 stimulates the expression and secretion of CTGF in human granulosa cells and provide evidence that both proteins may play critical roles in the regulation of extracellular matrix formation in these cells. PMID: 27392496
  16. These studies identify distinctive structural features of GDF11 that enhance its potency, relative to GDF8; however, the biological consequences of these differences remain to be determined. PMID: 28257634
  17. Serum myostatin levels were significantly decreased in heart failure patients and associated with lower extremity muscle wasting. PMID: 27390974
  18. our data showed a virtual absence of the variant (K) allele in MSTN rs1805086 in Japanese population, and no differences in allele/genotype frequencies in ACTN3 rs1815739 among centenarians and healthy controls of this country. PMID: 27861536
  19. MSTN, but not GDF11, declines in healthy men throughout aging. PMID: 27304512
  20. Multivariate regression analysis revealed that myostatin levels correlated significantly with tricuspid annular plane systolic excursion values and right ventricle myocardial performance index among the study patients PMID: 27323660
  21. Study measured circulating myostatin levels in seven inherited muscle diseases using an immunoaffinity LC-MS/MS approach, found significantly lower serum myostatin concentrations in numerous muscle disease patient populations and the associations with clinical measurements suggests the potential utility of myostatin as a biomarker of genetic muscle disease progression PMID: 28074267
  22. data indicated that serum myostatin concentration did not correlate with muscle and bone mass in postmenopausal women PMID: 27144806
  23. Myostatin mRNA expression in skeletal muscle was significantly reduced compared with pre-exercise values at all time points with no difference between exercise intensity. PMID: 27467217
  24. Low expression of serum MSTN is associated with Cachexia Prevention in Patients with Medullary Thyroid Cancer. PMID: 27165248
  25. Myostatin was differentially expressed in the muscle and adipose tissue in relation to physical activity and dysglycaemia PMID: 26572800
  26. Our results suggest that serum levels of myostatin and irisin are related in patients with type 2 diabetes PMID: 26438394
  27. Matrix metalloproteinase 14 was highly expressed in uterine leiomyoma and correlated with myostatin and activin A mRNA expression. Moreover, MMP14 and myostatin mRNA expression correlated significantly and directly with the intensity of dysmenorrhea. PMID: 26138721
  28. A synthetic peptide corresponding to this decorin region dose-dependently inhibited the response to myostatin in cardiomyocytes PMID: 27559042
  29. Findings suggest that GDF8 and CTGF may play critical roles in the regulation of proliferative events in human granulosa cells. PMID: 26577677
  30. Myostatin concentrations in plasma and protein expression in placental tissue are significantly higher in women with preeclampsia. Cytokine production by first-trimester placental tissues was altered following myostatin treatment. PMID: 25736326
  31. Myostatin is a well-described negative regulator of postnatal skeletal and cardiac muscle mass and modulates metabolic processes. It functions in the heart, skeletal muscle, and brain. Review. PMID: 27034275
  32. Plasma myostatin levels are increased in chronic obstructive pulmonary disease patients who have cor pulmonale. PMID: 26998756
  33. In human granulosa cells, GDF8 may play an important role in the modulation of cellular responsiveness to gonadotropins and in the regulation of ovarian steroid production, most likely as a luteinization inhibitor. PMID: 26607022
  34. Plasma myostatin might be suitable in predicting weight regain after marked weight loss, but no association with weight loss was observed in patients undergoing a non-surgical weight loss program. PMID: 26393401
  35. Plasma MSTN level was elevated in an early stage of CKD, which could be involved in the progression of sarcopenia. PMID: 26502079
  36. it is becoming clearer that besides its conventional role in muscle, myostatin plays a critical role in metabolism. Hence, molecular mechanisms by which myostatin regulates several key metabolic processes need to be further explored. PMID: 26305594
  37. The aim of this study was to investigate MSTN polymorphisms in an elderly sarcopenic population in Turkey and determine how they relate to sarcopenia. PMID: 26046327
  38. There is a significant decrease in levels of circulating myostatin in postsurgical acute phase reaction. PMID: 25749570
  39. Myostatin myocardial expression increases in the presence of structural cardiomyopathy either of hypertensive or other origin. PMID: 25915890
  40. In contrast to elite endurance and power track and field athletes, the MSTN 153RR genotype was not found in short distance-swimmers, and among the long distance-swimmers it was not associated with top level swimming performance. PMID: 25936293
  41. Lower serum myostatin independently associated with MetS, central obesity, low HDL-C, and high triglycerides after adjustment. Higher serum myostatin is associated with favorable metabolic profiles. PMID: 25254550
  42. Results indicate that GDF8 down-regulates PTX3 expression via SMAD-dependent signaling in human granulosa cells suggesting a potential role for GDF8 in the regulation of follicular function. PMID: 25641196
  43. Adenomyotic tissues express high levels of myostatin, follistatin, and activin type II receptors. PMID: 26086422
  44. This is the first demonstration of a spatial asymmetry in the expression pattern of Mstn/IGF-I in healthy hearts, which is likely to play a role in the different growth regulation of left vs. right ventricle PMID: 25591711
  45. Myostatin expression in placental tissue is altered under stress conditions (e.g. obesity and abnormal glucose metabolism) found in pregnancies complicated with gestational diabetes mellitus. PMID: 25443639
  46. we show that the K153R mutation significantly increases the rate of proteolysis of promyostatin by furin, but has no effect on the activity of the latent complex or the cleavage of the latent complex by bone morphogenetic protein 1 (BMP-1). PMID: 25543063
  47. MyoD acts to promote SC proliferation and transition of cells into differentiation, while myogenin is known to drive terminal differentiation PMID: 25108351
  48. role of myostatin in cardiovascular disease and cachexia PMID: 24680839
  49. Myostatin localization was positively identified in extravillous trophoblast. Myostatin positively affected proliferation and migration of extravillous trophoblast. PMID: 25093622
  50. The present study demonstrated that the variant alleles of MSTN A55T and K153R polymorphisms could significantly enhance muscle hypertrophy in response to strength training among Han Chinese men. PMID: 24479661
  51. the variant allele of the MSTN K153R polymorphism could be among the genetic contributors associated with exceptional longevity. PMID: 23354683
  52. The increase in myostatin and IGF-1 levels in Pompe disease patients may reflect muscle regeneration after ERT PMID: 23967261
  53. Our results indicate that a down regulation of skeletal muscle mRNA content of myostatin occurs after an aerobic exercise and weight loss program in obese men and women. PMID: 23687104
  54. Data indicate that muscle MYOG mRNA expression doubled, whereas MSTN protein expression decreased following immobilization. PMID: 24215591
  55. Hyperammonemia in cirrhosis induces transcriptional regulation of myostatin by an NF-kappaB-mediated mechanism. PMID: 24145431
  56. Myostatin mRNA levels are downregulated in gastric cancer patients who have minimal or no weight loss. PMID: 23861230
  57. Data suggest that MSTN binding to its receptor (activin receptor type II) is blocked by WFIKKN1 (growth and differentiation factor-associated serum protein-2); various forms of myostatin (promyostatin, processed myostatin) bind WFIKKN1 (not WFIKKN2). PMID: 23829672
  58. These results strongly suggest that myostatin plays an important role in regulation of myoblast differentiation from iPS cells of human origin. PMID: 23291166
  59. Resistance exercise resulted in a significant downregulation of MSTN and FBXO32 mRNA expression and a significant upregulation in FSTL3 and SMURF1 mRNA expression, and carbohydrate and protein feeding have little influence on the these markers expression. PMID: 22476926
  60. report that variants of Myostatin gene predispose to obesity, abdominal obesity and low lean body mass in Asian Indians in north India PMID: 22916099
  61. discussion of role of myostatin as key regulator of placental formation, development, and function; myostatin is biosynthesized as precursor protein and is active as dimer [REVIEW] PMID: 22818745
  62. In older men,total myostatin serum levels are inversely correlated with abdominal aortic calcification . PMID: 22968486
  63. In men, circulating myostatin levels show seasonal changes and are associated with age, body mass index, fat mass, smoking, and 25OHD levels. PMID: 22802085
  64. Myostatin may have a role in abdominal obesity, androgen and follistatin levels in women with polycystic ovary syndrome PMID: 22693174
  65. Data suggest that serum levels of myostatin are higher in pre-eclampsia than in controls; placental expression of myostatin is also increased in pre-eclampsia compared with controls; no differences were observed between mild and severe pre-eclampsia. PMID: 22568578
  66. high muscular expression of myostatin is associated to impaired metabolism, systemic inflammation, obesity and poor fitness level in healthy subjects. These associations are disrupted in patients with type 2 diabetes PMID: 22615949
  67. Report a significant reduction of myostatin in skeletal muscle after 12 weeks of exercise training in patients with chronic heart failure. PMID: 21450574
  68. After eccentric exercise, postmenopausal women not using hormone therapy (HT) expressed lower levels of myostatin while postmenopausal women using HT showed a heightened response. PMID: 22395277
  69. Suggest that genetic variation within myostatin may play a role in regulating the variation in fat mass in Chinese males. PMID: 22426697
  70. MSTN inhibit the adipogenesis of muscle satellite cells and adipose-derived stem cells. PMID: 22081442
  71. LIR was able to induce gains in 1RM and quadriceps CSA similar to those observed after traditional HI. These responses may be related to the concomitant decrease in MSTN and increase in FLST isoforms, GASP-1, and SMAD-7 mRNA gene expression. PMID: 21900845
  72. In a study of a Spanish and 2 North African cohorts, frequency of the AMPD1 C34T mutation was lower in Berbers compared with the Alpujarra cohort. The GDF8 K153R substitution showed little variability among the three cohorts. PMID: 22324844
  73. Myostatin activity was higher in alcoholic myocardium than in controls, mainly in those with cardiomyopathy. PMID: 21463333
  74. Common variants of MSTN and CKM genes don't play a role in attaining high level endurance performance in Caucasians. PMID: 20536908
  75. Our data firstly suggest that genetic variant rs3791783 A/G in myostatin gene are associated with obesity. PMID: 22209717
  76. The mode of dialyzer modulates the blood levels of myostatin. Higher myostatin is associated with lower muscle function. PMID: 21605155
  77. Structure of myostatin.follistatin-like 3: N-terminal domains of follistatin-type molecules exhibit alternate modes of binding. PMID: 22052913
  78. Myostatin expression in increased in congenital heart disease PMID: 21931616
  79. we clarified the role of myostatin in regulating myotube size and muscle protein degradation in human skeletal muscle PMID: 21900687
  80. human rhabdomyosarcoma RD cell differentiation is regulated by reciprocal, functional interactions between myostatin, p38 and extracellular regulated kinase signalling pathways PMID: 21273059
  81. Older individuals may paradoxically possess more favorable resistance exercise response regarding myostatin pathway-related genes and protein marker of pathway activity. PMID: 21665986
  82. myostatin blocks human myoblast proliferation by regulating cell cycle progression through targeted upregulation of p21 PMID: 21508334
  83. The exon skipping level, transcriptional expression of myostatin and its target genes, and combined myostatin and several dystrophin antisense oligonucleotides. PMID: 21507246
  84. MSTN K153R polymorphism is associated with the ability to produce 'peak' power during muscle contractions, as assessed with vertical jump tests, in young non-athletic men. PMID: 21283721
  85. Investigation of myostatin serum levels before and after a 6-month lifestyle intervention program in obese children. PMID: 21104587
  86. Accelerated apoptosis and upregulation of myostatin are observed in the skeletal muscle of patients with chronic kidney disease at an advanced stage. PMID: 21228768
  87. Effects of training and androgens on MYOPRO and FOLLI concentrations in blood and skeletal muscle, were investigated. PMID: 20801187
  88. These results strongly suggest that myostatin can play an essential role in the adaptation of skeletal muscle to hypoxic environment. PMID: 20884321
  89. analysis of quadriceps myostatin expression in COPD PMID: 20930205
  90. Increased myostatin mRNA in the paretic thigh and reduction with resistive training imply an important regulatory role for myostatin after stroke. PMID: 21164115
  91. Physical and laboratory examinations revealed that true haploinsufficiency of COL3A1, COL5A2, and MSTN, but not that of SLC40A1, leads to a clinical phenotype. PMID: 20648054
  92. heterozygosity for the GDF8 K153R polymorphism does not seem to exert a negative influence on the muscle phenotypes PMID: 20640547
  93. Results show that the myostatin precursor protein is capable of forming amyloid structures in vitro with implications for a role in sIBM pathogenesis. PMID: 20161792
  94. data suggest that myostatin is a pro-fibrogenic factor that enhances cellular proliferation and extracellular matrix synthesis by ACL fibroblasts. PMID: 20186835
  95. Creatine supplementation in conjunction with resistance training lead to greater decreases in serum myostatin (p<0.05), but had not additional effect on GASP-1 (p>0.05). PMID: 20026378
  96. in 2 families with myotonia congenita, no variants in MSTN gene were found; results show that the hypermusculature associated with myotonia in these families could not be attributed to modulation of muscle growth by variation in myostatin expression PMID: 19918890
  97. Deficiency of this protein in mice affects skeletal muscle and bone weight and development. PMID: 12060865
  98. Insulin-like growth factor-1 and myostatin mRNA expression in muscle: comparison between 62-77 and 21-31 yr old men PMID: 12175483
  99. negative regulation in vivo by myostatin propeptide and follistatin-related gene PMID: 12194980
  100. myostatin might represent an important regulator of skeletal muscle size also in conditions of food restriction in obese subjects. PMID: 15181048

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Involvement in disease Muscle hypertrophy (MSLHP)
Subcellular Location Secreted
Protein Families TGF-beta family
Database Links

HGNC: 4223

OMIM: 601788

KEGG: hsa:2660

STRING: 9606.ENSP00000260950

UniGene: Hs.41565

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