Human Napsin-A(NAPSA) ELISA kit

Code CSB-EL015452HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
napsin A aspartic peptidase
Alternative Names
Asp 4 ELISA Kit; ASP4 ELISA Kit; Aspartyl protease 4 ELISA Kit; KAP ELISA Kit; Kdap ELISA Kit; Kidney derived aspartic protease like protein ELISA Kit; NAP1 ELISA Kit; NAPA ELISA Kit; Napsa ELISA Kit; NAPSA_HUMAN ELISA Kit; Napsin 1 ELISA Kit; napsin A aspartic peptidase ELISA Kit; Napsin A precursor ELISA Kit; Napsin-1 ELISA Kit; Napsin-A ELISA Kit; Pronapsin A ELISA Kit; SNAPA ELISA Kit; TA01/TA02 ELISA Kit
Abbreviation
NAPSA
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates
Detection Range
4.7 ng/mL-300 ng/mL
Sensitivity
1.18 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cell Biology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human NAPSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:5 Average % 104
Range % 97-108
1:10 Average % 101
Range % 95-104
1:20 Average % 97
Range % 89-101
1:40 Average % 106
Range % 98-110
Recovery
The recovery of human NAPSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 101 95-104
EDTA plasma (n=4) 101 94-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
300 2.435 2.527 2.481 2.289
150 2.042 2.104 2.073 1.881
75 1.455 1.409 1.432 1.240
37.5 0.843 0.823 0.833 0.641
18.75 0.552 0.538 0.545 0.353
9.4 0.381 0.376 0.379 0.187
4.7 0.301 0.311 0.306 0.114
0 0.190 0.194 0.192  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human NAPSA ELISA Kit was designed for the quantitative measurement of Human NAPSA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.7 ng/mL-300 ng/mL and the sensitivity is 1.18 ng/mL.

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Target Background

Function
(From Uniprot)
May be involved in processing of pneumocyte surfactant precursors.
Gene References into Functions
  1. These data indicated that napsin A expression may inhibit TGFbetalinduced EMT and was negatively associated with EMTmediated erlotinib resistance, suggesting that napsin A expression may improve the sensitivity of lung cancer cells to EGFRTKI through the inhibition of EMT. PMID: 29845258
  2. Although napsin A is infrequently expressed in endometrial carcinomas, positive results of napsin A immunostaining in endometrial neoplasms might support the diagnosis of clear cell carcinoma when the pathologic differential diagnosis includes other histologic subtypes PMID: 26945446
  3. it may be useful to combine NAPA and TTF-1 for increased sensitivity in lung cancer diagnostics. There is no substantial difference between monoclonal and polyclonal p40 and between different NAPA clones, whereas there is a difference between the TTF-1 clones 8G7G3/1 and SPT24 PMID: 26447895
  4. To the best of our knowledge, expression of monoclonal Napsin A in lymphomas has never been reported. ALK-DLBCL should be considered in the differential diagnosis when evaluating a Napsin A-positive tumor of poorly differentiated morphology and of unknown primary PMID: 26808134
  5. It is important for pathologists to be aware that breast carcinomas with apocrine features can express napsin A. PMID: 26842346
  6. High Napsin A expression is associated with adenocarcinoma in non-small cell lung carcinoma. PMID: 27412420
  7. Napsin-A, and Desmocollin-3 were sensitive and specific markers for the diagnosis of AC and SCC, respectively. Both markers allowed classification of 54/60 cases into either AC or SCC. PMID: 26710975
  8. napsin A is aberrantly expressed in a subset of lymphomas PMID: 26400099
  9. CK7, TTF-1 and napsin A are predominantly expressed in primary lung adenocarcinoma patients, with CDX-2 being inconsistently expressed. PMID: 26469326
  10. Combining HNF-1beta and napsin A may distinguish clear cell carcinoma from high-grade serous carcinoma, endometrioid adenocarcinoma and metastatic Krukenberg tumors. PMID: 26339401
  11. napsin A is another sensitive and specific marker for distinguishing ovarian clear cell tumors (especially adenocarcinomas) from other ovarian tumors PMID: 24721826
  12. Napsin A is expressed in a broad spectrum of renal neoplasms. PMID: 25889632
  13. Low expression levels of NAPSA is associated with lung adenocarcinoma. PMID: 25982999
  14. In diagnosis of ovarian clear cell carcinoma, Napsin A is specific but of intermediate sensitivity. PMID: 25551297
  15. Napsin A is frequently expressed in ovarian and endometrial clear cell carcinomas. PMID: 25971546
  16. Data indicate that polyclonal but not monoclonal napsin A antibody has a virtually universal nonspecific labeling in mucinous adenocarcinomas of various sites. PMID: 25521803
  17. These data suggest that napsin A may be a useful marker for identifying metastatic adenocarcinomas of pulmonary origin PMID: 24479710
  18. Our study showed that napsin A is an extremely sensitive (100%) marker of ovarian clear cell carcinomas PMID: 25389337
  19. Napsin A as a marker of clear cell ovarian carcinoma. PMID: 24191930
  20. PAX2 and napsin A have high specificity but low sensitivity and only have limited value in the differential diagnosis of mesotheliomas and renal cell carcinomas PMID: 23503645
  21. TTF-1 is more sensitive than napsin for detection of lung sarcomatoid carcinoma, and no cases were positive for napsin but negative for TTF-1 PMID: 24331839
  22. Napsin A is a sensitive and specific biomarker of the clear cell histotype in endometrial carcinomas and accordingly may have diagnostic utility in their histotyping. PMID: 24145649
  23. 24 cases each of pulmonary and esophageal adenocarcinoma were stained with TTF-1, napsin A, CDX2, 34betaE12, N-cadherin, and IMP3 in an attempt to find an optimal panel for differentiation. IMP3, CDX2, and N-cadherin are superior to either TTF-1 or napsin A. PMID: 23899066
  24. Napsin-A seems to be a useful marker in classifying primary pulmonary neoplasm as adenocarcinomas. PMID: 22914608
  25. A minority of anaplastic and poorly differentiated micropapillary pattern thyroid carcinomas are napsin A positive. PMID: 23681073
  26. Napsin B was duplicated from napsin A during the early stages of primate evolution, and the subsequent loss of napsin B function during primate evolution reflected ongoing human-specific napsin B pseudogenization PMID: 23333608
  27. Mucin-producing neoplasms of the lung infrequently express napsin A, suggesting that immunohistochemical assessment of napsin A may have limited diagnostic usefulness for distinguishing primary and metastatic mucinous adenocarcinomas involving the lung. PMID: 23355200
  28. investigation of expression of NAPSA (a potential diagnostic marker) in lungs/respiratory mucosa of subjects with pulmonary sclerosing hemangioma PMID: 23194051
  29. a useful immunohistochemical marker for differentiation of lung squamous cell carcinoma and adenocarcinoma from other subtypes PMID: 22495379
  30. absence of napsin A was an independent prognostic factor for reduced survival time in lung adenocarcinoma PMID: 22418245
  31. Nap-A was more specific than thyroid transcription factor 1 for primary lung adenocarcinoma versus all tumors, excluding kidney, independent of tumor type PMID: 22288963
  32. None of the 90 squamous cell carcinomas of the lung exhibited napsin A positivity in the neoplastic cells; however, strong napsin A reactivity was observed in hyperplastic type II pneumocytes and in intra-alveolar macrophages entrapped within the tumor. PMID: 22198009
  33. napsin A is a useful marker that can assist in the diagnosis of both lung adenocarcinomas and renal cell carcinomas[review] PMID: 22156835
  34. in combination with thyroid transcription factor-1-positive immunostaining helps in differentiating primary lung adenocarcinoma from metastatic carcinoma in the lung PMID: 21464700
  35. Although TTF-1 had a higher sensitivity, napsin-A was useful as a surrogate marker when encountering a poorly differentiated lung adenocarcinoma. PMID: 20830690
  36. Pronapsin A gene expression in normal and malignant human lung and mononuclear blood cells PMID: 12151090
  37. Napsin is a promising marker for the diagnosis of primary lung adenocarcinoma. PMID: 12698189
  38. role in the N- and C-terminal processing of pro-surfactant protein-B in type-II pneumocytes PMID: 13129928
  39. NAPSA suppresses tumor growth independent of its catalytic activity. PMID: 18195689
  40. A general defect in napsin A or cathepsin H expression or activity was not the specific cause for abnormal surfactant accumulation in juvenile pulmonary alveolar proteinosis PMID: 18216060
  41. The combined use of napsin A and thyroid transcription factor-1 results in improved sensitivity and specificity for identifying pulmonary adenocarcinoma in primary lung tumors and in a metastatic setting. PMID: 19740516

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Subcellular Location
Secreted.
Protein Families
Peptidase A1 family
Tissue Specificity
Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes.
Database Links

HGNC: 13395

OMIM: 605631

KEGG: hsa:9476

STRING: 9606.ENSP00000253719

UniGene: Hs.512843

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