Human Phosphatase and actin regulator 1(PHACTR1) ELISA kit

Code CSB-EL017880HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
phosphatase and actin regulator 1
Alternative Names
PHACTR1 ELISA Kit; KIAA1733 ELISA Kit; RPEL1 ELISA Kit; Phosphatase and actin regulator 1 ELISA Kit
Abbreviation
PHACTR1
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
23.5 pg/mL-1500 pg/mL
Sensitivity
5.86 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PHACTR1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 83-97
1:2 Average % 103
Range % 95-107
1:4 Average % 90
Range % 82-94
1:8 Average % 99
Range % 90-102
Recovery
The recovery of human PHACTR1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 85-96
EDTA plasma (n=4) 101 91-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1500 2.051 1.936 1.994 1.866
750 1.277 1.244 1.261 1.133
375 0.755 0.741 0.748 0.620
187.5 0.427 0.405 0.416 0.288
94 0.315 0.307 0.311 0.183
47 0.252 0.243 0.248 0.120
23.5 0.182 0.176 0.179 0.051
0 0.127 0.129 0.128  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human PHACTR1 ELISA Kit was designed for the quantitative measurement of Human PHACTR1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.

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Target Background

Function
(From Uniprot)
Binds actin monomers (G actin) and plays a role in multiple processes including the regulation of actin cytoskeleton dynamics, actin stress fibers formation, cell motility and survival, formation of tubules by endothelial cells, and regulation of PPP1CA activity. Involved in the regulation of cortical neuron migration and dendrite arborization.
Gene References into Functions
  1. our study suggests that several PHACTR1 and SLC22A3 gene polymorphisms may exert a protective effect against the CAD in the Chinese Han male population. PMID: 27893421
  2. PHACTR1 is a key atherosclerosis candidate gene since it is regulated by atherogenic stimuli in macrophages and endothelial cells PMID: 27187934
  3. Stimulation of Slack K(+) channels alters mass at the plasma membrane by triggering dissociation of Phactr-1. PMID: 27545877
  4. CRISPR-edited stem cell-derived endothelial cells demonstrate rs9349379, a common SNP in the 3 intron of the PHACTR1 gene, regulates expression of endothelin 1 (EDN1), a gene located 600 kb upstream of PHACTR1. The known physiologic effects of EDN1 on the vasculature may explain the pattern of risk for the five associated vascular diseases. PMID: 28753427
  5. To evaluate the role of two polymorphisms (rs2026458 and rs9349379) of the PHACTR1 gene in the susceptibility to the risk of developing premature coronary artery disease (CAD) in the Mexican population. Results suggest that the PHACTR1 rs9349379 polymorphism plays an important role in the risk of developing premature CAD in the Mexican population. PMID: 27517945
  6. the expression of PHACTR1 by genotypes in primary human fibroblasts showed higher expression in rs9349379-A carriers . Phactr1 knockdown in zebrafish resulted in dilated vessels indicating subtle impaired vascular development PMID: 27792790
  7. There was no significant association between the presence of risk alleles of rs12526453 and coronary heart disease in an Iranian population. PMID: 28287809
  8. our result highlighted the pivotal role of phactr-1 protein in the pathogenesis of atherosclerosis. PMID: 26362351
  9. The rs12526453 CC homozygotes (previously associated with increased risk of myocardial infarction) showed, in 2 independent samples, better long-term survival. PMID: 26086777
  10. GWAS analysis of 1,393 cervical artery dissection cases and 14,416 controls showed that the rs9349379[G] allele was associated with lower risk. This was confirmed independent follow-up samples. PMID: 25420145
  11. a novel signaling route whereby TGF-beta silences the expression of miR-584, resulting in enhanced PHACTR1 expression, and further leading to actin rearrangement and breast cancer cell migration. PMID: 23479725
  12. 3 loci from related cardiovascular genomewide studies were significant: PHACTR1 in large-vessel disease (P=2.63e(-6)), PITX2 in cardioembolic stroke (P=4.78e(-8)), and ZFHX3 in cardioembolic stroke (P=5.50e(-7)). PMID: 23042660
  13. Results from this GWAS support a central role of PHACTR1 in CAD susceptibility irrespective of lifestyle and ethnic divergences. PMID: 22745674
  14. Phactr-1 is a key component in the angiogenic process PMID: 21939755
  15. Findings suggest that PHACTR-1 is likely to be a key regulator of endothelial cell function properties. PMID: 21798305
  16. Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator) PMID: 20738937
  17. Observational study of gene-disease association. (HuGE Navigator) PMID: 20971364
  18. Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
  19. Observational study and genome-wide association study of gene-disease association. (HuGE Navigator) PMID: 19198609

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Subcellular Location
Cytoplasm. Cell junction, synapse. Nucleus.
Protein Families
Phosphatase and actin regulator family
Tissue Specificity
Detected in umbilical vein endothelial cells.
Database Links

HGNC: 20990

OMIM: 608723

KEGG: hsa:221692

STRING: 9606.ENSP00000329880

UniGene: Hs.436996

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