PHACTR1 Antibody

Datasheet

Code	CSB-PA017880LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, Rat brain tissue, Mouse brain tissue All lanes: PHACTR1 antibody at 6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 67, 55 kDa Observed band size: 67 kDa IHC image of CSB-PA017880LA01HU diluted at 1:400 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA017880LA01HU diluted at 1:400 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of A549 cells with CSB-PA017880LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PHACTR1 Polyclonal antibody

Uniprot No.

Q9C0D0

Target Names

PHACTR1

Alternative Names

PHACTR1 antibody; KIAA1733 antibody; RPEL1 antibody; Phosphatase and actin regulator 1 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human Phosphatase and actin regulator 1 protein (263-387AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PHACTR1 Antibody (Product code: CSB-PA017880LA01HU) is Non-conjugated. For PHACTR1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA017880LB01HU	PHACTR1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA017880LC01HU	PHACTR1 Antibody, FITC conjugated
Biotin	CSB-PA017880LD01HU	PHACTR1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Binds actin monomers (G actin) and plays a role in multiple processes including the regulation of actin cytoskeleton dynamics, actin stress fibers formation, cell motility and survival, formation of tubules by endothelial cells, and regulation of PPP1CA activity. Involved in the regulation of cortical neuron migration and dendrite arborization.

Gene References into Functions

our study suggests that several PHACTR1 and SLC22A3 gene polymorphisms may exert a protective effect against the CAD in the Chinese Han male population. PMID: 27893421
PHACTR1 is a key atherosclerosis candidate gene since it is regulated by atherogenic stimuli in macrophages and endothelial cells PMID: 27187934
Stimulation of Slack K(+) channels alters mass at the plasma membrane by triggering dissociation of Phactr-1. PMID: 27545877
CRISPR-edited stem cell-derived endothelial cells demonstrate rs9349379, a common SNP in the 3 intron of the PHACTR1 gene, regulates expression of endothelin 1 (EDN1), a gene located 600 kb upstream of PHACTR1. The known physiologic effects of EDN1 on the vasculature may explain the pattern of risk for the five associated vascular diseases. PMID: 28753427
To evaluate the role of two polymorphisms (rs2026458 and rs9349379) of the PHACTR1 gene in the susceptibility to the risk of developing premature coronary artery disease (CAD) in the Mexican population. Results suggest that the PHACTR1 rs9349379 polymorphism plays an important role in the risk of developing premature CAD in the Mexican population. PMID: 27517945
the expression of PHACTR1 by genotypes in primary human fibroblasts showed higher expression in rs9349379-A carriers . Phactr1 knockdown in zebrafish resulted in dilated vessels indicating subtle impaired vascular development PMID: 27792790
There was no significant association between the presence of risk alleles of rs12526453 and coronary heart disease in an Iranian population. PMID: 28287809
our result highlighted the pivotal role of phactr-1 protein in the pathogenesis of atherosclerosis. PMID: 26362351
The rs12526453 CC homozygotes (previously associated with increased risk of myocardial infarction) showed, in 2 independent samples, better long-term survival. PMID: 26086777
GWAS analysis of 1,393 cervical artery dissection cases and 14,416 controls showed that the rs9349379[G] allele was associated with lower risk. This was confirmed independent follow-up samples. PMID: 25420145
a novel signaling route whereby TGF-beta silences the expression of miR-584, resulting in enhanced PHACTR1 expression, and further leading to actin rearrangement and breast cancer cell migration. PMID: 23479725
3 loci from related cardiovascular genomewide studies were significant: PHACTR1 in large-vessel disease (P=2.63e(-6)), PITX2 in cardioembolic stroke (P=4.78e(-8)), and ZFHX3 in cardioembolic stroke (P=5.50e(-7)). PMID: 23042660
Results from this GWAS support a central role of PHACTR1 in CAD susceptibility irrespective of lifestyle and ethnic divergences. PMID: 22745674
Phactr-1 is a key component in the angiogenic process PMID: 21939755
Findings suggest that PHACTR-1 is likely to be a key regulator of endothelial cell function properties. PMID: 21798305
Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator) PMID: 20738937
Observational study of gene-disease association. (HuGE Navigator) PMID: 20971364
Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 20379614
Observational study and genome-wide association study of gene-disease association. (HuGE Navigator) PMID: 19198609

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Subcellular Location

Cytoplasm. Cell junction, synapse. Nucleus.

Protein Families

Phosphatase and actin regulator family

Tissue Specificity

Detected in umbilical vein endothelial cells.

Database Links

HGNC: 20990

OMIM: 608723

KEGG: hsa:221692

STRING: 9606.ENSP00000329880

UniGene: Hs.436996

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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