Human Soluble protein-100,S-100 ELISA Kit

Code CSB-E08067h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P23297
Lead Time 3-5 working days
Abbreviation S100A1
Target Name S100 calcium binding protein A1
Alias S100, S100-alpha, S100A, S100 alpha|S100 calcium-binding protein A1|S100 protein, alpha polypeptide
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies.
HGNC 10486
RGD 3614
MGI 1338917
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human S-100 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 90-96  
1:2 Average % 102  
Range % 97-107  
1:4 Average % 87  
Range % 82-90  
1:8 Average % 100  
Range % 97-103  
Recovery
The recovery of human S-100 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 89-98  
EDTA plasma (n=4) 97 90-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 2.401 2.438 2.420 2.284  
5 1.892 1.871 1.882 1.746  
2.5 1.212 1.234 1.223 1.087  
1.25 0.661 0.698 0.680 0.544  
0.625 0.364 0.384 0.374 0.238  
0.312 0.283 0.261 0.272 0.136  
0.156 0.206 0.213 0.210 0.074  
0 0.135 0.137 0.136    
References
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Function Probably acts as a Ca(2+) signal transducer
Subcellular Location Cytoplasm
Protein Families S-100 family
Tissue Specificity Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
Database Links

HGNC: 10486

OMIM: 176940

KEGG: hsa:6271

STRING: 9606.ENSP00000292169

UniGene: Hs.515715

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