Human Sulfotransferase 1A1(SULT1A1) ELISA kit

Instructions
Code CSB-EL022933HU
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1
Alternative Names Aryl sulfotransferase 1 ELISA Kit; HAST1/HAST2 ELISA Kit; Human aryl sulfotransferase mRNA complete cds ELISA Kit; MGC131921 ELISA Kit; MGC5163 ELISA Kit; OTTHUMP00000162568 ELISA Kit; OTTHUMP00000162569 ELISA Kit; P PST 1 ELISA Kit; P PST ELISA Kit; P-PST 1 ELISA Kit; Phenol sulfating phenol sulfotransferase 1 ELISA Kit; Phenol sulfotransferase 1 ELISA Kit; Phenol-sulfating phenol sulfotransferase 1 ELISA Kit; PST ELISA Kit; ST1A1 ELISA Kit; ST1A1_HUMAN ELISA Kit; ST1A3 ELISA Kit; STP ELISA Kit; STP1 ELISA Kit; Sulfotransferase 1A1 ELISA Kit; Sulfotransferase family 1A phenol preferring member 1 ELISA Kit; Sulfotransferase family cytosolic 1A phenol preferring member 1 ELISA Kit; Sulfotransferase phenol preferring 1 ELISA Kit; SULT1A1 ELISA Kit; Thermostable phenol sulfotransferase ELISA Kit; Thermostable phenol sulfotransferase1 ELISA Kit; Ts-PST ELISA Kit; TSPST1 ELISA Kit
Abbreviation SULT1A1
Uniprot No. P50225
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 25 pg/mL-1600 pg/mL
Sensitivity 6.25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SULT1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 85
Range % 80-89
1:2 Average % 103
Range % 98-107
1:4 Average % 88
Range % 84-92
1:8 Average % 98
Range % 94-102
Recovery
The recovery of human SULT1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 85-95
EDTA plasma (n=4) 95 90-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1600 2.078 2.148 2.113 1.918
800 1.512 1.468 1.490 1.295
400 1.004 1.020 1.012 0.817
200 0.733 0.689 0.711 0.516
100 0.496 0.511 0.504 0.309
50 0.381 0.374 0.378 0.183
25 0.275 0.286 0.281 0.086
0 0.197 0.192 0.195  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitters. Has also estrogen sulfotransferase activity. responsible for the sulfonation and activation of minoxidil. Is Mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could so participate as modulating factor of cancer risk.
Gene References into Functions
  1. SULT1A1 Arg213His (rs9282861) polymorphism might be associated with breast cancer risk, especially among Asian population. PMID: 29233949
  2. No significant difference was observed in the RNA levels of CYP1A1 and SULT1A1 between the two groups. The frequency of expression of the CYP17 T/C variant tended to be higher and the A allele of COMT polymorphism together with down-regulation of its mRNA expression may be more frequent in Chinese women with idiopathic POI PMID: 28887105
  3. The importance of SULT1A1 genotype for hepatic methyleugenol DNA adducts in humans, and a strong impact of SULT1A1 copy number variations on SULT1A1 hepatic phenotype. PMID: 28326452
  4. SULT1A1 role in the metabolic detoxification of heterocyclic aromatic amines PMID: 28160022
  5. Silencing the SULT1A1 gene led to changes in resveratrol metabolism, with higher intracellular accumulation of the nonmetabolized resveratrol. PMID: 28523759
  6. the NQO1 Pro187Ser or SULT1A1 Arg213His polymorphism combination with smoking significantly confer susceptibility to BC. [META-ANALYSIS] PMID: 28589969
  7. SULT1A1 gene copy number affected the minor allele frequency for each single nucleotide polymorphisms tested. Before administration of exogenous hormones, increasing number of G alleles at rs9282861 was associated with earlier age at menopause, lower frequency of night sweats, and less severe insomnia. Variability in onset of menopause and symptoms before initiation of hormone therapy is partly due to SULT1A1 variation. PMID: 27300114
  8. SULT1A1 copy number variation was negatively correlated with estrone-sulfate to estrone ratio predominantly in males (E1S/E1; p=0.03, r=-0.21) and may be associated with increased risk for common allergies. PMID: 28867356
  9. This study demonstrates that the presence of His allele and Gln allele in case of SULT1A1 rs9282861 and XRCC1 rs25487, respectively, involve in lung cancer prognosis in Bangladeshi population. PMID: 29110586
  10. We conclude from these studies that SULT1A1 is involved in the bioactivation of AA-I through the sulfonation of AL-I-NOH, contributing significantly to the toxicities of AA observed in vivo. PMID: 27207664
  11. Epigallocatechin gallate (EGCG) displays high affinity and specificity for SULT1A1. The allosteric network is shown to involve 14 distinct complexes. ECGG binds both the allosteric site and, relatively weakly, the active site of SULT1A1. PMID: 27356022
  12. The polymorphism of SULT1A1*2 is not associated with esophageal squamous cell carcinoma risk. PMID: 26455829
  13. SULT1A1 high Copy Number Variation on high Estrogen Concentration and Tamoxifen-Associated Adverse Drug Reactions in Premenopausal Thai Breast Cancer. PMID: 27221864
  14. SULT1A1 is responsible for bioactivation of food genotoxicants 5-hydroxymethylfurfural and furfuryl alcohol. PMID: 25370010
  15. Cytosolic sulfotransferase 1A1 regulates HIV-1 minus-strand DNA elongation in primary human monocyte-derived macrophages. PMID: 26906565
  16. A systematic analysis showed that three of the twelve human SULTs, SULT1A1, SULT1A3 and SULT1C4, displayed the strongest sulphating activity towards acetaminophen. PMID: 26067475
  17. Higher SULT1A1 levels were observed in rats as well as in humans exposed to high altitude, when compared to sea-level controls PMID: 26022216
  18. The present study provided epidemiological evidence for a significantly increased risk of UCB in ever smokers with the Ala/Ala genotype of the GSTO1 gene and the Arg/Arg genotype of the SULT1A1 gene. PMID: 25103078
  19. Sulfotransferase 1A1 Substrate Selectivity: A Molecular Clamp Mechanism. PMID: 26340710
  20. The aim of the study was to assess whether selected single nucleotide polymorphisms of CYP1A1 and 2E1, GSTM1, GSTT1, and SULT1A1 influence susceptibility towards hepatocellular carcinoma. PMID: 25654087
  21. Results indicate that SULT1A1 Arg(213)His may act as a low-penetrance risk allele for developing MBC and could be associated with a specific tumor subtype associated with HER2 overexpression. PMID: 25385181
  22. Sulfo-conjugation of the multi-hydroxylated metabolites of benzene by human SULT1A1 may represent an important detoxifying pathway. PMID: 25771868
  23. the SULT1A1 Arg213His polymorphism may contribute UADT cancer risk, but didn't show any association with breast cancer. PMID: 25225888
  24. that SULT1A1 Arg213His polymorphism is associated with bladder cancer risk. PMID: 25194687
  25. 3'-Phosphoadenosine 5'-phosphosulfate binds antisynergistically to the subunits of the SULT1A1 dimer. PMID: 25314023
  26. The results of this meta-analysis indicate that the SULT1A1 Arg213His polymorphism is associated with the risk bladder cancer under a recessive model. PMID: 24763827
  27. The SNP rs9282861 with GG genotype of SULT1A1 was associated with an elevated risk of total neural tube defects. PMID: 24307569
  28. The SULT1A1 genetic variability is associated with cancer risk and response to therapy (review). PMID: 24010997
  29. Association between BRCA2 mutation and SULT1A1 gene deletion in male breast cancer emerged. PMID: 23711090
  30. SULT1A1 variant allele increases breast cancer risk among subjects who were exposed to high smoked meat intake. PMID: 23157889
  31. The functional significance of the single nucleotide polymorphisms/haplotypes located upstream of the SULT1A1 start codon. PMID: 23080433
  32. discussion of possible association between lower-activity of SULT1A1 with sudden cardiac death (both holiday sudden cardiac death in older adults and sudden infant death syndrome) [REVIEW] PMID: 22678655
  33. We observed a previously unreported association between the SULT1A1 rs9282861 genotype and overall survival of breast cancer patients treated with adjuvant chemotherapy or tamoxifen. PMID: 22708928
  34. Arg213His polymorphism is not associated with lung cancer. PMID: 22524828
  35. SULT1A1 Arg213His polymorphism is associated with breast cancer. PMID: 22011087
  36. Women in Siberia with SNPS in CYP1A1 gene, in CYP1A2 gene,and in the SULT1A1 gene have an increased risk of development of breast cancer . PMID: 21977969
  37. SULT1A1 Arg213His polymorphism, ethnicity, and smoking may modulate environment-related cancer risk. PMID: 21670965
  38. Stp1 is important for appropriate regulation of Stk1 function, hemolysin activity, autolysis, and GBS virulence PMID: 22081606
  39. This meta-analysis demonstrates that there is no association between the SULT1A1 R213H polymorphism and colorectal cancer. PMID: 21695180
  40. SULT1A1 1/2 does not contribute to the variation in SULT1A1 enzymatic activity when the 3'-UTR SNPs are included in the statistical model PMID: 20881232
  41. mechanism of SULT1A1-catalyzed sulfation of adenosine 3',5'-diphosphate by para-nitrophenyl sulfate PMID: 21111704
  42. Only NAT1 showed a significant lower DNA methylation rate in the control group than in the tamoxifen-resistant breast cancer group, and no significant difference in methylation was found in COMT, CYP1A1, CYP2D6, and SULT1A1 genes. PMID: 20628863
  43. Polymorphism of SULT1A1 Arg213His is associated with breast cancer. PMID: 20663177
  44. Meta-analysis did not find a significant general relationship between SULT1A1 R213H polymorphism & the risk of breast cancer, but ethnic population analysis revealed a significantly increased breast cancer risk for HH allele carriers among Asians. PMID: 19949855
  45. The interaction between SULT1A1 and CYP1A2 can play an important role in hepatocarcinogenesis in the Chinese population. PMID: 19906068
  46. study demonstrates that the loss of SULT1A1 appears to be a characteristic molecular signature of hepatocellular carcinoma. PMID: 19904771
  47. human CYP2E1 and SULT1A1 activate an endogenous cellular molecule or a medium component to become mutagenic PMID: 19484729
  48. The high activity SULT1A1*1 allozyme protects against dietary and/or environmental chemicals involved in the pathogenesis of colorectal cancer. PMID: 11692076
  49. genetic polymorphism in SULT1A1 gene may be associated with increased lung cancer risk PMID: 11804685
  50. 7-OH-flavone sulfotransferase followed Michaelis-Menten kinetics PMID: 12162852

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Subcellular Location Cytoplasm
Protein Families Sulfotransferase 1 family
Tissue Specificity Liver, lung, adrenal, brain, platelets and skin.
Database Links

HGNC: 11453

OMIM: 171150

KEGG: hsa:6817

STRING: 9606.ENSP00000321988

UniGene: Hs.567342

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