Human Tumor necrosis factor receptor superfamily member 6B(TNFRSF6B) ELISA kit

Instructions
Code CSB-EL023982HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name tumor necrosis factor receptor superfamily, member 6b, decoy
Alternative Names DcR3 ELISA Kit; Decoy receptor 3 ELISA Kit; Decoy receptor for Fas ligand ELISA Kit; DJ583P15.1.1 ELISA Kit; M68 ELISA Kit; M68E ELISA Kit; OTTHUMP00000031583 ELISA Kit; TNF6B_HUMAN ELISA Kit; TNFRSF6B ELISA Kit; TR6 ELISA Kit; Tumor necrosis factor receptor superfamily member 6B ELISA Kit; Tumor necrosis factor receptor superfamily member 6b decoy ELISA Kit; UNQ186/PRO212 ELISA Kit
Abbreviation TNFRSF6B
Uniprot No. O95407
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TNFRSF6B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 95  
Range % 87-99  
1:10 Average % 89  
Range % 82-93  
1:20 Average % 91  
Range % 88-95  
1:40 Average % 97  
Range % 91-102  
Recovery
The recovery of human TNFRSF6B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 82-97  
EDTA plasma (n=4) 98 90-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 3.002 2.987 2.995 2.893  
5 2.182 2.113 2.148 2.046  
2.5 1.413 1.444 1.429 1.327  
1.25 0.658 0.666 0.662 0.560  
0.625 0.362 0.378 0.370 0.268  
0.312 0.219 0.214 0.217 0.115  
0.156 0.138 0.131 0.135 0.033  
0 0.100 0.104 0.102    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

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Target Data

Function Decoy receptor that can neutralize the cytotoxic ligands TNFS14/LIGHT, TNFSF15 and TNFSF6/FASL. Protects against apoptosis.
Gene References into Functions
  1. The combination of decoy receptor 3, soluble urokinase type plasminogen activator receptor, and procalcitonin improved the sensitivity and specificity of diagnosis of sepsis, suggesting that use of the combination of three indexes enhanced the efficiency of sepsis diagnosis. PMID: 29760745
  2. Compared with the control, miR-340 was significantly lower in the serum of hepatocellular carcinoma patients (p<0.01). miR-340 was lower in HCC tissues than in adjacent; however, DcR3, TGF-beta1 and Smad2 were higher. PMID: 29311025
  3. DcR3 treatment caused HepG2 cell cytoskeleton remodeling, inhibited E-cadherin expression, and promoted cell migration. PMID: 28560426
  4. DcR3 gene polymorphisms are associated with sporadic breast infiltrating ductal carcinoma risk in Northeast Chinese females PMID: 27517320
  5. Study found that both transcriptional expression and post-transcriptional DcR3 expression DcR3 expression were significantly up-regulated in colorectal cancer (CRC) tissues. DcR3 was found to be playing a critical role in CRC proliferation and migration in vitro and in tumorigenesis and metastasis in vivo. PMID: 27764793
  6. Higher plasma DcR3 level was related to better coronary collateral circulation (CCC) formation and displayed potent predictive power for CCC status in patients with coronary artery disease. PMID: 28942284
  7. High serum DcR3 levels are associated with disease severity in nonatopic asthma patients. PMID: 26911723
  8. Results show that DcR3 engages TNF ligands: FasL, LIGHT, and TL1A in a structurally similar fashion and its promiscuity is due to recognition of backbone atoms and invariant side chains. PMID: 27806260
  9. when idiopathic pulmonary fibrosis cells interact with collagen matrix, aberrantly activated Akt increases DcR3 expression via GSK-3beta-NFATc1 and protects IPF cells from the FasL-dependent apoptotic pathway. PMID: 27218286
  10. Coexpression of TNFRSF6B and PCDHGA3 was observed immunohistochemically in FL18 cells, suggesting potential cooperation in tumor cell maintenance. PMID: 27748813
  11. Loss of DcR3 impaired the growth and invasive property of HCC cell line of HepG2. PMID: 27779171
  12. Overexpression of DcR3 was significantly related to the risk of female reproductive cancers (OR=10.69, 95% CI: 6.33-18.05), TNM stage (OR=5.51, 95% CI: 2.83-10.71), differentiation (OR=4.16, 95% CI: 2.28-7.60), lymph node metastasis (OR=5.89, 95% CI: 3.16-10.9), age (OR=0.85, 95% CI: 0.51-1.44), and overall survival time (OR=1.84, 95% CI: 0.58-5.83). PMID: 27246752
  13. The serum level of DcR3 may be a useful marker for disease activity in ANCA-associated renal vasculitis. PMID: 27251675
  14. The sDcR3 levels in patients with HIV-1 subtype B were significantly higher than those in patients infected with subtype CRF01_AE. HIV-1 subtype B and slow disease progression were associated with higher levels of sDcR3. PMID: 26062966
  15. DcR3 inhibits influenza a virus-induced JNK and ERK activation in human macrophages PMID: 25940317
  16. Decoy receptor 3 (DcR3) expression in kidneys has been shown to predict progression of chronic kidney disease. PMID: 26335204
  17. Decoy receptor 3 regulates the expression of tryptophan hydroxylase 1 in rheumatoid synovial fibroblasts PMID: 26238767
  18. tumour markers DcR3 and growth/differentiation factor (GDF)15 from 100 mul human serum, were quantified. PMID: 25823874
  19. Serum DcR3 expression in primary Sjogren's syndrome (pSS) patients was significantly higher than healthy controls. DcR3 expression in salivary glands of pSS patients was significantly higher than healthy controls. PMID: 25564309
  20. study has defined the increased serum and SF samples levels of TL1A and DcR3 in patients with rheumatoid arthritis (RA); findings support the hypothesis that TL1A and DcR3 may contribute to the pathogenesis of RA PMID: 25647275
  21. decoy receptor 3 and geriatric nutrition have a role in mortality in chronic hemodialysis patients PMID: 25323840
  22. Higher DcR3 expression was related to the status of invasion, lymph node metastasis and recurrence in bladder urothelial carcinoma. PMID: 25422191
  23. HER2 overexpression is not an independent prognostic marker of colorectal cancer, but DcR3 overexpression is highly correlated with lymph node metastasis and poor overall survival PMID: 24764685
  24. The overexpression of DcR3 might play a crucial role in the tumorigenesis, differentiation, and proliferation of glioma. PMID: 24741354
  25. Overexpression of TNFRSF6B is associated with hepatocellular carcinoma. PMID: 24998573
  26. A case-control study of esophageal squamous-cell carcinoma (ESCC)found that the DcR3 SNPs were not associated with the risk of ESCC. PMID: 24786982
  27. Inhibition of B cell functions by DcR3 may partially explain the negative correlation between DcR3 level and disease activity in rheumatoid arthritis patients. PMID: 25084482
  28. High serum DcR3 expression correlated with lymph node metastases in in pancreatic head carcinoma PMID: 24597666
  29. DcR3 is a key driver of tumor cell dissemination. PMID: 24107265
  30. DcR3 may play an etiologic role in systemic lupus erythematosus through either elevated serum levels of wild-type DcR3 or normal levels of gain-of-function DcR3 proteins that increase lymphocyte proliferation. PMID: 23729807
  31. Rare missense mutations in TNFRSF6B have a role in the pathogenesis of some cases of IBD. PMID: 23965943
  32. DCR3 is overexpressed in breast cancer and in vascular endothelial cells and tumor cells in the lymph nodes, revealing a possible link with lymphangiogenesis. PMID: 24612949
  33. Silencing of decoy receptor 3 (DcR3) expression by siRNA in pancreatic carcinoma cells induces Fas ligand-mediated apoptosis in vitro and in vivo. PMID: 23846297
  34. Our results implied the therapeutic potential of silencing DcR3 expression by RNAi in colon cancer. PMID: 24101127
  35. The findings in this study strongly suggest that DcR3 plays important roles in tumor progression of human MFH by decoy as well as non-decoy functions and that DcR3 may serve as a potent therapeutic target for human MFH. PMID: 23817777
  36. Serum DcR3 level may be useful for detecting Hepatitis Be Antigen (-) Chronic hepatitis B in the active stage, which requires medical treatment. PMID: 23925044
  37. DcR3 might regulate the gene expression of various key molecules in rheumatoid arthritis fibroblast-like synoviocytes by binding to TL1A. PMID: 23912906
  38. Data suggest DcR3 expression in DSV-PTC (diffuse sclerosing variant of papillary thyroid carcinoma) tumor cells promotes polarized macrophage differentiation toward the M2 phenotype and thus has role in recruitment of tumor-associated macrophages. PMID: 23186064
  39. high expression of TNFRSF6B is a novel biomarker for predicting the progression of chronic kidney disease PMID: 23449012
  40. DcR3 expression was associated with other prognostic factors including survivin, reduced remission rates, and shorter event-free survival. PMID: 23652586
  41. novel regulatory mechanisms of DcR3 expression in psoriasis, particularly in keratinocytes and endothelial cells, provides new insight into the pathogenesis of psoriasis PMID: 23707413
  42. DcR3 and ERK1/2 play a vital role in the development of gastric cancer, and they may be new markers for indicating the efficiency of gastric cancer treatment in the future. PMID: 22672288
  43. Higher DcR3 levels strongly correlate with inflammation and independently predict cardiovascular and all-cause mortality in CKD patients on hemodialysis. PMID: 22626963
  44. High expression and amplification of the DcR3 gene is associated with pancreatic carcinoma. PMID: 22524850
  45. These results imply that DcR3 may regulate B cell activation by suppressing the activation of NF-kappaB. PMID: 22581854
  46. The increased synovial inflammatory cells infiltration in rheumatoid arthritis and ankylosing spondylitis was associated with the elevated DCR3 expression. PMID: 22325083
  47. Significantly enhanced tumor growth and spreading are observed in DcR3-transgenic mice; DcR3-transfectant cells grow faster and result in infiltration by tumor-associated macrophages. PMID: 22287720
  48. The expression of DcR3 in vascular endothelial cells during the early phases of Kaposi's sarcoma-associated herpesvirus infection ensures the successful establishment and maintenance of the viral infection. PMID: 21293109
  49. DcR3-Fc inhibits the proliferation induced by TNFalpha or IL-1beta in rheumatoid arthritis. PMID: 21537832
  50. EMS1 expression was positively related to DcR3, which might play an important role in the carcinogenesis and development of laryngeal carcinoma. PMID: 21395182

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Subcellular Location Secreted
Tissue Specificity Detected in fetal lung, brain and liver. Detected in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung. Highly expressed in several primary tumors from colon, stomach, rectum, esophagus and in SW480 colon carcinoma cells.
Database Links

HGNC: 11921

OMIM: 603361

KEGG: hsa:8771

STRING: 9606.ENSP00000359013

UniGene: Hs.434878

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