Human Tumor necrosis factor receptor superfamily member 6B(TNFRSF6B) ELISA kit

Code CSB-EL023982HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O95407
Lead Time 5-7 working days
Abbreviation TNFRSF6B
Protein Biological Process 1 Apoptosis/Autophagy
Target Name tumor necrosis factor receptor superfamily, member 6b, decoy
Alias hCG_2044836, DCR3, DJ583P15.1.1, M68, TR6, OTTHUMP00000031583|decoy receptor 3|tumor necrosis factor receptor superfamily, member 6b
Species Homo sapiens (Human)
Protein Biological Process 3 Apoptosis
Sample Types serum, plasma, cell culture supernates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene belongs to the tumor necrosis factor receptor superfamily. The encoded protein is postulated to play a regulatory role in suppressing FasL- and LIGHT-mediated cell death. It acts as a decoy receptor that competes with death receptors for ligand binding. Overexpression of this gene has been noted in gastrointestinal tract tumors, and it is located in a gene-rich cluster on chromosome 20, with other potentially tumor-related genes. Two transcript variants encoding the same isoform, but differing in the 5 UTR, have been observed for this gene.
HGNC 11921
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TNFRSF6B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 95  
Range % 87-99  
1:10 Average % 89  
Range % 82-93  
1:20 Average % 91  
Range % 88-95  
1:40 Average % 97  
Range % 91-102  
Recovery
The recovery of human TNFRSF6B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 82-97  
EDTA plasma (n=4) 98 90-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
10 3.002 2.987 2.995 2.893  
5 2.182 2.113 2.148 2.046  
2.5 1.413 1.444 1.429 1.327  
1.25 0.658 0.666 0.662 0.560  
0.625 0.362 0.378 0.370 0.268  
0.312 0.219 0.214 0.217 0.115  
0.156 0.138 0.131 0.135 0.033  
0 0.100 0.104 0.102    
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Function Decoy receptor that can neutralize the cytotoxic ligands TNFS14/LIGHT, TNFSF15 and TNFSF6/FASL. Protects against apoptosis.
Subcellular Location Secreted
Tissue Specificity Detected in fetal lung, brain and liver. Detected in adult stomach, spinal cord, lymph node, trachea, spleen, colon and lung. Highly expressed in several primary tumors from colon, stomach, rectum, esophagus and in SW480 colon carcinoma cells.
Database Links

HGNC: 11921

OMIM: 603361

KEGG: hsa:8771

STRING: 9606.ENSP00000359013

UniGene: Hs.434878

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