Human Urotensin Ⅱ ELISA kit

Instructions
Code CSB-E12057h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name urotensin 2
Alternative Names PRO1068 ELISA Kit; U-II ELISA Kit; UCN2 ELISA Kit; UII ELISA Kit; Urotensin 2 preproprotein isoform a ELISA Kit; Urotensin 2 preproprotein isoform b ELISA Kit; Urotensin II ELISA Kit; Urotensin-2 ELISA Kit; UTS2 ELISA Kit; UTS2_HUMAN ELISA Kit; UTSII ELISA Kit
Abbreviation UTS2
Uniprot No. O95399
Species Homo sapiens (Human)
Sample Types serum, saliva, cerebrospinal fluid (CSF), tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human urotensinⅡ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 97  
Range % 90-106  
1:2 Average % 94  
Range % 86-98  
1:4 Average % 87  
Range % 82-96  
1:8 Average % 91  
Range % 95-97  
Recovery
The recovery of human urotensinⅡ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
20 2.930 2.898 2.914 2.809  
10 2.160 2.008 2.084 1.979  
5 1.565 1.482 1.524 1.419  
2.5 1.005 0.999 1.002 0.897  
1.25 0.517 0.512 0.515 0.410  
0.625 0.337 0.321 0.329 0.224  
0.312 0.247 0.228 0.238 0.133  
0 0.107 0.103 0.105    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Background

Function
(From Uniprot)
Highly potent vasoconstrictor.
Gene References into Functions
  1. polymorphisms of the UTS2 gene are not risk factors for migraine in our sample of Turkish migraine patients. PMID: 28686849
  2. Host genetics influences UTS2 levels, which may contribute to inflammation and cardiovascular damage in KD PMID: 28753597
  3. Opposite actions of urotensin II and relaxin-2 on cellular expression of fibronectin in renal fibrosis PMID: 28613403
  4. Ser89Asn (S89N) polymorphisms of the UTS2 gene were associated with hypertension in a Chinese female population. Additionally, we demonstrated that genotype Asn89Asn was associated with left ventricular posterior wall thickness for subjects with hypertension and cardiac hypertrophy in a Chinese female population. PMID: 27941008
  5. Children and adolescents with CKD, particularly those who are not on hemodialysis (HD) and renal transplantation, have significantly higher levels of UII than healthy subjects. UII levels increase significantly at the end of the HD session. The presence of metabolic acidosis affects significantly plasma UII levels. PMID: 28359257
  6. UII/GPR14 signaling was involved in the DSS-induced colonic inflammation and its inhibition may serve as a potential therapeutic target, which may be associated with the NF-kappaB signaling pathway. PMID: 27600191
  7. Ser89Asn polymorphisms of the UTS2 gene are significantly associated with atrial fibrillation in the Chinese population. Additionally, we demonstrated that genotype Met21Met may have a potential beneficial role in atrial fibrillation. PMID: 26811505
  8. Serum urotensin II levels were significantly lower in hemodialysis patients compared to healthy subjects. PMID: 26870964
  9. expression of UII is associated with ERS in patients with SPE. Our results indicate that UII may trigger ERS in placental trophoblastic cells in patients with preeclampsia. PMID: 25880595
  10. Our study suggests that U-II may play a role in migraine pathogenesis; also Thr21Met polymorphism was associated with the risk of migraine disease. PMID: 27090416
  11. Used transgenic rabbits as a model to study macrophage-specific expressing human UII (hUII) and studied the role of autocrine UII in the development of atherosclerosis. PMID: 26640798
  12. Urotensin II-increased reactive oxygen species production via the NADPH oxidase pathway is partially associated with activation of the PI3K/Akt and ERK cascades PMID: 26658815
  13. provide evidence that, in glioma cells, homogeneous concentration of UII allowed translocation of Galpha13 to the UT receptor at the plasma membrane and increased actin stress fibers, lamellipodia formation and vinculin-stained focal adhesions PMID: 25597409
  14. enzyme-linked immunosorbent assay (ELISA) and restriction fragment length polymorphism analysis were used to evaluate plasma levels of urotensin-II and Thr21Met and Ser89Asn polymorphisms of UTS2 gene in breast cancer patients. PMID: 25604143
  15. Urotensin II can induce the proliferation of BEL-7402 cells via the urotensin receptor mediated PKC/ERK/p38 MAPK signaling pathways. PMID: 25514221
  16. Urotensin-II induces activating transcription factor 3 (ATF3) at both mRNA and protein levels. PMID: 25268131
  17. The significantly high Urotensin-II levels in the knee synovial fluid of osteoarthritic patients than in non-osteoarthritic patients. PMID: 24468547
  18. This study revealed high plasma U-II level might be accepted as a risk factor for in-stent restenosis with bare metal stent PMID: 24831766
  19. Urotensin II seems be associated with menopausal status, and extra-nodal and lymphatic invasion in breast cancer patients. PMID: 25112588
  20. Study suggests that soluble CD40 ligand and urotensin II likely play a role in the pathogenesis of slow coronary flow. PMID: 25648034
  21. Plasma urotensin II levels are elevated in patients with acute coronary syndrome. PMID: 24642358
  22. U-II induces IL-8 expression in human umbilical vein endothelial cells via p38 mitogen-activated protein kinase and extracellular signal-regulated kinase signaling pathways. PMID: 24587311
  23. U-II level was higher in schizophrenia patients than in controls. PMID: 24552634
  24. results indicated that urotensin II might be an independent risk factor for essential hypertension. PMID: 24339964
  25. Elevation or urotensin II in the serum of preeclampsia patients could be corrected to the severity and/or progression of the disease. The urotensin genotype frequency between patients and control groups showed a significant difference. PMID: 25134283
  26. family-based analysis of association between blood pressure, glomerular filtration and genes of the urotensin-II pathway (urotensin-II, urotensin-II related peptide, urotensin-II receptor) saturated with 28 tagging single nucleotide polymorphisms PMID: 24391740
  27. mutation associated with pre-eclampsia risk PMID: 23905608
  28. Serum urotensin II levels were higher in patients with non-dipper hypertension than in patients with dipper hypertension (204 [106-533] vs. 140 [96-309]. There was a positive correlation between total systolic blood pressure and serum urotensin II levels. PMID: 23301552
  29. Data suggest that both urotensin II (UTS2) and C-reactive protein (CRP) are elevated in sera of subjects with polycystic ovary syndrome; UTS2 may play role in physiopathology of insulin resistance and increased cardiovascular events in such patients. PMID: 23981122
  30. Thr21Met (T21M) but not Ser89Asn (S89N) polymorphisms of the urotensin-II (UTS-II) gene are associated with Behcet's disease in a Turkish population. PMID: 23333481
  31. Data suggest that aortic urotensin II (UII) is increased in diabetes-associated atherosclerosis in humans and mice; diabetes-associated plaque development is attenuated by UII/UII receptor antagonism in in vitro (human) and in vivo (mouse) settings. PMID: 23344731
  32. Urotensin II was expressed in the lung of pulmonary hypertension patients with congenital heart disease. PMID: 23158820
  33. Associations between Thr21Met and Ser89Asn polymorphisms in the UTS2 gene and diabetic retinopathy strongly suggest that these polymorphisms may be an important a risk factor in Caucasians. PMID: 22587369
  34. U-II elicited penile erection through eNOS activation PMID: 22319601
  35. High urotensin II levels in recipients of kidney transplants could be drug-related (immunosuppressive drugs) and may be of practical importance that may be used to improve the long-term outcome of the patients PMID: 22098077
  36. Data show an association between Thr21Met, but not Ser89Asn, in the urotensin II gene and systemic sclerosis. PMID: 22045841
  37. Increased UT-II, sTM, and vWF in ankylosing spondylitis patient sera regardless of treatment and disease activity suggest an increased tendency for atherosclerosis. PMID: 21556780
  38. In Korean women, the common UTS-II 143 G/A polymorphism is unlikely to have an association with the risk of preeclampsia. PMID: 21623998
  39. polymorphisms in UTS2 and PER3 may have roles in glucose homeostasis and diabetes PMID: 21559414
  40. U-II levels in serum are significantly increased in children with chronic liver disease (portal hypertension or liver cirrhosis) compared with healthy controls; magnitude of U-II increase is related to severity and long-term liver disease outcomes. PMID: 21694541
  41. Urotensin-II may have a vital role in systemic sclerosis. PMID: 21274584
  42. the precursor undergoes pH- and Ca(2+)-dependent conformational and stability changes; ProUII has a stable tertiary structure at pH 5.5 in presence of Ca(2+) and is correctly cleaved in these conditions by prohormone convertases. PMID: 21605557
  43. circulating urotensin II may participate in the worsening course of some type 2 diabetes mellitus patients. PMID: 20695946
  44. This study suggest that UTS2 single gene (S89N) polymorphism is not associated with pre-eclampsia. PMID: 20653105
  45. elevated in plasma of pre-eclamptic women PMID: 20017703
  46. The expression of urotensin 2 is increased in airways of rat asthmatic models and of patients with asthma. PMID: 17045018
  47. These findings suggest that the intrahepatic UII/UT system has an important pathophysiological role in cirrhosis and portal hypertension. PMID: 20428787
  48. Endothelin-1 and urotensin-II levels are increased in plasma of patients with coronary heart disease. PMID: 20339975
  49. The U-II, in addition to the well known role in the regulation of cardiovascular function, also exert a specific angiogenic activity. PMID: 19362580
  50. Urocortin II exhibits motor suppressive and delayed anxiolytic-like effects, suggesting a time-dependent role in the regulation of stress-related behavior. PMID: 12088848

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Subcellular Location Secreted
Protein Families Urotensin-2 family
Tissue Specificity Brain specific.
Database Links

HGNC: 12636

OMIM: 604097

KEGG: hsa:10911

UniGene: Hs.715862

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