CUSABIO’s Human antidiuretic hormone/vasopressin/arginine vasopressin (ADH/VP/AVP) ELISA kit is a sandwich immunoassay specifically designed and validated for the quantitative detection of AVP in the serum, plasma, or tissue homogenates. It is not intended for diagnostic use. This assay kit was designed and optimized for neuroscience research use in humans. The kit has undergone rigorous quality control in multiple parameters, including sensitivity, specificity, precision, linearity, recovery, and inter-batch difference. Refer to the product instructions for more details.
This assay employs the quantitative sandwich enzyme immunoassay technique, in which AVP in the samples or standards are sandwiched between pre-coated AVP antibody and Biotin-conjugated AVP antibody. HRP-avidin is then added into the wells. Following a wash to remove any unbound reagent, the TMB substrate solution is added to the wells and color develops in proportion to the amount of AVP bound in the initial step. The color development is stopped upon adding the stop solution, and the intensity of the color is measured at 450 nm via a microplate reader. The levels of AVP in the samples can be determined by referring to the O.D. (optical density) of the samples to the standard curve.
AVP is a peptide hormone that is synthesized in the hypothalamus, and it travels via the axon-to-posterior pituitary-to-circulation routine. It plays essential roles in the control of the body’s osmotic balance, blood pressure regulation, sodium homeostasis, and kidney functioning. The maintenance of osmolality facilitates to sustain the volume of water in the extracellular fluid, which is necessary to protect cells from a sudden increase or decrease in water content thus ensuring proper cell function. Some AVP may be released into the brain from the hypothalamus and may affect social behavior, sexual motivation and pair bonding, and maternal responses to stress. Additionally, AVP induces stem cells to differentiate into cardiomyocytes and promotes heart muscle homeostasis.